Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes.

abstract：:The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

journal_title：Applied and environmental microbiology

authors： Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

更新日期：1995-11-01 00:00:00

abstract：:Water samples were collected from four locations on two rivers in Washington State and analyzed by membrane filtration-immunofluorescence assay to establish Cryptosporidium oocyst concentrations. Sampling locations were selected to evaluate effects of watershed character, from pristine mountain to downstream agricultu...

journal_title：Applied and environmental microbiology

authors： Hansen JS,Ongerth JE

更新日期：1991-10-01 00:00:00

abstract：:This study quantified the levels of airborne microorganisms in six swine farms with more than 10,000 pigs in subtropical Taiwan. We evaluated breeding, growing, and finishing stalls, which were primarily open-air buildings, as well as partially enclosed farrowing and nursery piggeries. Airborne culturable bacteria, gr...

journal_title：Applied and environmental microbiology

authors： Chang CW,Chung H,Huang CF,Su HJ

更新日期：2001-01-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：:Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

journal_title：Applied and environmental microbiology

authors： Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

更新日期：2011-08-01 00:00:00

abstract：UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

journal_title：Applied and environmental microbiology

authors： Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

更新日期：2016-08-15 00:00:00

abstract：:Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Caputo RA,Graham GS

更新日期：1981-06-01 00:00:00

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:Infections caused by Toxoplasma gondii are widely prevalent in animals and humans throughout the world. In the United States, an estimated 23% of adolescents and adults have laboratory evidence of T. gondii infection. T. gondii has been identified as a major opportunistic pathogen in immunocompromised individuals, in ...

journal_title：Applied and environmental microbiology

authors： Schwab KJ,McDevitt JJ

更新日期：2003-10-01 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:The internal transcribed spacer (ITS) of the gene coding for rRNA was sequenced in both directions with the gene walking technique in a black morel (Morchella conica) and a yellow morel (M. esculenta) to elucidate the ITS length discrepancy between the two species groups (750-bp ITS in black morels and 1,150-bp ITS in...

journal_title：Applied and environmental microbiology

authors： Wipf D,Munch JC,Botton B,Buscot F

更新日期：1996-09-01 00:00:00

abstract：:Although carbon dioxide (CO(2)) is known to inhibit growth of most bacteria, very little is known about the cellular response. The food-borne pathogen Listeria monocytogenes is characterized by its ability to grow in high CO(2) concentrations at refrigeration temperatures. We examined the listerial responses of differ...

journal_title：Applied and environmental microbiology

authors： Jydegaard-Axelsen AM,Høiby PE,Holmstrøm K,Russell N,Knøchel S

更新日期：2004-07-01 00:00:00

abstract：:The alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of...

journal_title：Applied and environmental microbiology

authors： Boucher I,Parrot M,Gaudreau H,Champagne CP,Vadeboncoeur C,Moineau S

更新日期：2002-12-01 00:00:00

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00

abstract：:Escherichia coli O157:H7 is a leading cause of food-borne illness. This human pathogen produces Shiga toxins (Stx1 and Stx2) which inhibit protein synthesis by inactivating ribosome function. The present study describes a novel cell-based assay to detect Stx2 and inhibitors of toxin activity. A Vero cell line harborin...

journal_title：Applied and environmental microbiology

authors： Quiñones B,Massey S,Friedman M,Swimley MS,Teter K

更新日期：2009-03-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

journal_title：Applied and environmental microbiology

authors： Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

更新日期：2012-08-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:Actinomycetes secrete into their surroundings a suite of enzymes involved in the biodegradation of plant lignocellulose; these have been reported to include both hydrolytic and oxidative enzymes, including peroxidases. Reports of secreted peroxidases have been based upon observations of peroxidase-like activity associ...

journal_title：Applied and environmental microbiology

authors： Mason MG,Ball AS,Reeder BJ,Silkstone G,Nicholls P,Wilson MT

更新日期：2001-10-01 00:00:00

abstract：:A novel S-adenosyl-L-methionine (SAM)-dependent methyltransferase catalyzing the O methylation of several chlorophenols and other halogenated phenols was purified 220-fold to apparent homogeneity from mycelia of Trichoderma longibrachiatum CECT 20431. The enzyme could be identified in partially purified protein prepar...

journal_title：Applied and environmental microbiology

authors： Coque JJ,Alvarez-Rodríguez ML,Larriba G

更新日期：2003-09-01 00:00:00

abstract：:Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

journal_title：Applied and environmental microbiology

authors： Inaba T,Mirocha CJ

更新日期：1979-01-01 00:00:00

abstract：:This study aimed at assessing the dynamics of lactic acid bacteria and other Firmicutes associated with durum wheat organs and processed products. 16S rRNA gene-based high-throughput sequencing showed that Lactobacillus, Streptococcus, Enterococcus, and Lactococcus were the main epiphytic and endophytic genera among l...

journal_title：Applied and environmental microbiology

authors： Minervini F,Celano G,Lattanzi A,Tedone L,De Mastro G,Gobbetti M,De Angelis M

更新日期：2015-10-01 00:00:00

abstract：:A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...

journal_title：Applied and environmental microbiology

authors： Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GC

更新日期：1996-08-01 00:00:00

abstract：:The harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as Australia, Germany, and South Africa, among others. However, conflicting reports on the microbial quality of harvested rainwater have been published. To monitor the presence of potential pathogenic bacteria durin...

journal_title：Applied and environmental microbiology

authors： Dobrowsky PH,De Kwaadsteniet M,Cloete TE,Khan W

更新日期：2014-04-01 00:00:00

abstract：:To study the anaerobic degradation of the chimera 3-chloro-4-hydroxybenzoate (3-Cl,4-OHB), anaerobic freshwater sediment samples from the vicinity of Athens, Ga., were adapted for the transformation of 4-hydroxybenzoate (4-OHB), 3-chlorobenzoate (3-CB), 2-chlorophenol (2-CP), and 2,4-dichlorophenol (2,4-DCP). In nonad...

journal_title：Applied and environmental microbiology

authors： Zhang X,Wiegel J

更新日期：1992-11-01 00:00:00

abstract：:Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Hedden P,Tudzynski B

更新日期：2008-12-01 00:00:00

abstract：:Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pP(x)GM(iii)) and a decrease in that of pP(x)GM(ii). The magnitude of (31)P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1-O-[N...

journal_title：Applied and environmental microbiology

authors： Park YI,Bonetti SJ,Gander JE

更新日期：2000-02-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentative...

journal_title：Applied and environmental microbiology

authors： Taller BJ,Wong TY

更新日期：1989-01-01 00:00:00

abstract：:The promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was used to drive expression of mnp1, the gene encoding Mn peroxidase isozyme 1, in primary metabolic cultures of Phanerochaete chrysosporium. A 1,100-bp fragment of the P. chrysosporium gpd promoter region was fused upstream of the mnp1 ge...

journal_title：Applied and environmental microbiology

authors： Mayfield MB,Kishi K,Alic M,Gold MH

更新日期：1994-12-01 00:00:00

abstract：:This study aimed at investigating the antifungal activity of Wickerhamomyces anomalus and sourdough lactic acid bacteria to extend the shelf life of wheat flour bread. The antifungal activity was assayed by agar diffusion, growth rate inhibition, and conidial germination assays, using Penicillium roqueforti DPPMAF1 as...

journal_title：Applied and environmental microbiology

authors： Coda R,Cassone A,Rizzello CG,Nionelli L,Cardinali G,Gobbetti M

更新日期：2011-05-01 00:00:00