Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.

abstract：:A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

journal_title：Applied and environmental microbiology

authors： Ishige T,Tani A,Sakai Y,Kato N

更新日期：2000-08-01 00:00:00

abstract：:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition as...

journal_title：Applied and environmental microbiology

authors： Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré J

更新日期：2012-06-01 00:00:00

abstract：:The lantibiotic (lanthionine-containing antibiotic) mersacidin is an antimicrobial peptide consisting of 20 amino acids and is produced by Bacillus sp. strain HIL Y-85,54728. The structural gene (mrsA) and the genes for producer self-protection, modification enzymes, transport proteins, and regulator proteins are orga...

journal_title：Applied and environmental microbiology

authors： Schmitz S,Hoffmann A,Szekat C,Rudd B,Bierbaum G

更新日期：2006-11-01 00:00:00

abstract：:We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) usi...

journal_title：Applied and environmental microbiology

authors： Lee J,Jurgenson JE,Leslie JF,Bowden RL

更新日期：2008-04-01 00:00:00

abstract：:A microscopically pure enrichment culture of a gram-negative anaerobic bacterium, in the present article referred to as PER-K23, was isolated from an anaerobic packed-bed column in which tetrachloroethene (PCE) was reductively transformed to ethane via trichloroethene (TCE), cis-1,2-dichloroethene (cis-1,2-DCE), chlor...

journal_title：Applied and environmental microbiology

authors： Holliger C,Schraa G,Stams AJ,Zehnder AJ

更新日期：1993-09-01 00:00:00

abstract：:Seven organotin compounds and tin chloride were tested for their effects on the methanogenic bacteria Methanococcus thermolithotrophicus, Methanococcus deltae delta LH, and Methanosarcina barkeri 227. The methanogens were strongly inhibited by triethyltin, tripropyltin, and monophenyltin compounds, generally at concen...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-04-01 00:00:00

abstract：:The membrane integrity of a cell is a well-accepted criterion for characterizing viable (active or inactive) cells and distinguishing them from damaged and membrane-compromised cells. This information is of major importance in studies of the function of microbial assemblages in natural environments, in order to assign...

journal_title：Applied and environmental microbiology

authors： Grégori G,Citterio S,Ghiani A,Labra M,Sgorbati S,Brown S,Denis M

更新日期：2001-10-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:beta-Aminoglutaric acid, a nonprotein amino acid isomer of glutamic acid, was found in the free amino acid pool of a marine bacterium, Alteromonas luteoviolacea. It was also found in a mixed culture of fermenting bacteria enriched from an anoxic marine sediment. ...

journal_title：Applied and environmental microbiology

authors： Henrichs SM,Cuhel R

更新日期：1985-08-01 00:00:00

abstract：:Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...

journal_title：Applied and environmental microbiology

authors： Williams JR,Morgan AG,Rouch DA,Brown NL,Lee BT

更新日期：1993-08-01 00:00:00

abstract：:Comparisons of 16S rDNA sequences were used to determine the phylogeny of not-yet-cultured spirochetes from hindguts of the African higher termite, Nasutitermes lujae (Wasmann). The 16S rRNA genes were amplified directly from spirochete-rich hindguts by using universal primers, and the amplified products were cloned i...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Dewhirst FE,Cooke SM,Fussing V,Poulsen LK,Breznak JA

更新日期：1996-02-01 00:00:00

abstract：:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

journal_title：Applied and environmental microbiology

authors： Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

更新日期：2012-09-01 00:00:00

abstract：:Two acetyl esterases (EC 3.1.1.6) were purified to gel electrophoretic homogeneity from Thermoanaerobacterium sp. strain JW/SL-YS485, an anaerobic, thermophilic endospore former which is able to utilize various substituted xylans for growth. Both enzymes released acetic acid from chemically acetylated larch xylan. Ace...

journal_title：Applied and environmental microbiology

authors： Shao W,Wiegel J

更新日期：1995-02-01 00:00:00

abstract：:Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...

journal_title：Applied and environmental microbiology

authors： Weyman PD,Pratte B,Thiel T

更新日期：2008-04-01 00:00:00

abstract：:On the basis of bioinformatic evidence, we suspected that proteins with a CYTH (CyaB thiamine triphosphatase) domain and/or a CHAD (conserved histidine α-helical domain) motif might represent polyphosphate (polyP) granule-associated proteins. We found no evidence of polyP targeting by proteins with CYTH domains. In co...

journal_title：Applied and environmental microbiology

authors： Tumlirsch T,Jendrossek D

更新日期：2017-03-17 00:00:00

abstract：:Streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. Serotyping is an important tool for detection and epidemiological studies of S. suis Thirty-three reference serotypes and nine novel cps loci (NCLs) are recognized in S. suis To gain a better understanding of the prevalence an...

journal_title：Applied and environmental microbiology

authors： Qiu X,Bai X,Lan R,Zheng H,Xu J

更新日期：2016-11-21 00:00:00

abstract：:Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

journal_title：Applied and environmental microbiology

authors： Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

更新日期：2001-09-01 00:00:00

abstract：:[This corrects the article on p. 514 in vol. 55.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1989-06-01 00:00:00

abstract：:The survival of Azospirillum brasilense Cd and Sp-245 in the rhizosphere of wheat and tomato plants and in 23 types of plant-free sterilized soils obtained from a wide range of environments in Israel and Mexico was evaluated. Large numbers of A. brasilense cells were detected in all the rhizospheres tested, regardless...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Puente ME,Rodriguez-Mendoza MN,Toledo G,Holguin G,Ferrera-Cerrato R,Pedrin S

更新日期：1995-05-01 00:00:00

abstract：UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

journal_title：Applied and environmental microbiology

authors： Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

更新日期：2016-08-15 00:00:00

abstract：:In a high-rate oxidation pond, 0.1 to 1.0% of the algal population of Scenedesmus obliquus was found to be infected by a chytrid. When suitable conditions developed, these infections burst into massive epidemics that killed most of the algae. The major factors triggering massive infections were optimal oxygen concentr...

journal_title：Applied and environmental microbiology

authors： Abeliovich A,Dikbuck S

更新日期：1977-12-01 00:00:00

abstract：:Five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and Klebsiella aerogenes. High-speed centrifugation was the most effective extraction method for the K. aerogenes culture, based on the ...

journal_title：Applied and environmental microbiology

authors： Brown MJ,Lester JN

更新日期：1980-08-01 00:00:00

abstract：:Perchlorate contamination can be microbially respired to innocuous chloride and thus can be treated effectively. However, monitoring a bioremediative strategy is often difficult due to the complexities of environmental samples. Here we demonstrate that microbial respiration of perchlorate results in a significant frac...

journal_title：Applied and environmental microbiology

authors： Coleman ML,Ader M,Chaudhuri S,Coates JD

更新日期：2003-08-01 00:00:00

abstract：:The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

journal_title：Applied and environmental microbiology

authors： Garcia-Pichel F,Wingard CE,Castenholz RW

更新日期：1993-01-01 00:00:00

abstract：:A vineyard isolate of the yeast Saccharomyces cerevisiae, UCD932, was identified as a strain producing little or no detectable hydrogen sulfide during wine fermentation. Genetic analysis revealed that this trait segregated as a single genetic determinant. The gene also conferred a white colony phenotype on BiGGY agar ...

journal_title：Applied and environmental microbiology

authors： Linderholm A,Dietzel K,Hirst M,Bisson LF

更新日期：2010-12-01 00:00:00

abstract：:Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocus...

journal_title：Applied and environmental microbiology

authors： Goller SP,Schoisswohl D,Baron M,Parriche M,Kubicek CP

更新日期：1998-09-01 00:00:00

abstract：:Although Salmonella enterica can produce life-threatening colitis in horses, certain serotypes are more commonly associated with clinical disease. Our aim was to evaluate the proportional morbidity attributed to different serotypes, as well as the phenotypic and genotypic antimicrobial resistance (AMR) of Salmonella i...

journal_title：Applied and environmental microbiology

authors： Leon IM,Lawhon SD,Norman KN,Threadgill DS,Ohta N,Vinasco J,Scott HM

更新日期：2018-06-18 00:00:00

abstract：:This is the first study to characterize the environmental conditions which contribute to the presence and proliferation of environmental mycobacteria in a major freshwater river. Over 20 different species of environmental mycobacteria were isolated, including the pathogenic M. avium and M. kansasii. Species of the rap...

journal_title：Applied and environmental microbiology

authors： Bland CS,Ireland JM,Lozano E,Alvarez ME,Primm TP

更新日期：2005-10-01 00:00:00

abstract：:The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

journal_title：Applied and environmental microbiology

authors： Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

更新日期：2005-09-01 00:00:00

abstract：:Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to gr...

journal_title：Applied and environmental microbiology

authors： van den Wijngaard AJ,van der Kamp KW,van der Ploeg J,Pries F,Kazemier B,Janssen DB

更新日期：1992-03-01 00:00:00