Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species.

Abstract:

:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.

journal_name

Appl Environ Microbiol

authors

Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré J

doi

10.1128/AEM.07591-11

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

4048-50

issue

11

eissn

0099-2240

issn

1098-5336

pii

AEM.07591-11

journal_volume

78

pub_type

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