Abstract:
:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré Jdoi
10.1128/AEM.07591-11subject
Has Abstractpub_date
2012-06-01 00:00:00pages
4048-50issue
11eissn
0099-2240issn
1098-5336pii
AEM.07591-11journal_volume
78pub_type
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