Appearance of enzyme activities catalyzing conversion of sterigmatocystin to aflatoxin B1 in late-growth-phase Aspergillus parasiticus cultures.

Abstract:

:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two activities into a postmicrosomal activity which catalyzed conversion of sterigmatocystin to O-methylsterigmatocystin and a microsomal activity which converted O-methylsterigmatocystin to aflatoxin B1. The two activities were absent in 24-h-old cells, increased to optimum levels during the stationary phase, and then declined.

journal_name

Appl Environ Microbiol

authors

Cleveland TE,Lax AR,Lee LS,Bhatnagar D

doi

10.1128/AEM.53.7.1711-1713.1987

subject

Has Abstract

pub_date

1987-07-01 00:00:00

pages

1711-3

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

53

pub_type

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