Abstract:
:The high risk associated with biological threat agents dictates that any suspicious sample be handled under strict surety and safety controls and processed under high-level containment in specialized laboratories. This study attempted to find a rapid, reliable, and simple method for the complete inactivation of a wide range of pathogens, including spores, vegetative bacteria, and viruses, while preserving microbial nucleic acid fragments suitable for PCRs and proteinaceous epitopes for detection by immunoassays. Formaldehyde, hydrogen peroxide, and guanidium thiocyanate did not completely inactivate high titers of bacterial spores or viruses after 30 min at 21°C. Glutaraldehyde and sodium hypochlorite showed high microbicidal activity but obliterated the PCR or enzyme-linked immunosorbent assay (ELISA) detection of bacterial spores or viruses. High-level inactivation (more than 6 log(10)) of bacterial spores (Bacillus atrophaeus), vegetative bacteria (Pseudomonas aeruginosa), an RNA virus (the alphavirus Pixuna virus), or a DNA virus (the orthopoxvirus vaccinia virus) was attained within 30 min at 21°C by treatment with either peracetic acid or cupric ascorbate with minimal hindrance of subsequent PCR tests and immunoassays. The data described here should provide the basis for quickly rendering field samples noninfectious for further analysis under lower-level containment and considerably lower cost.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Sagripanti JL,Hülseweh B,Grote G,Voss L,Böhling K,Marschall HJdoi
10.1128/AEM.05553-11subject
Has Abstractpub_date
2011-10-01 00:00:00pages
7289-95issue
20eissn
0099-2240issn
1098-5336pii
AEM.05553-11journal_volume
77pub_type
杂志文章abstract::The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...
journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.65.5.2202-2208.1999
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.33.1.59-64.1977
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.55.2.520-522.1989
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
doi:10.1128/AEM.46.3.758-761.1983
更新日期:1983-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.5.1837-1844.1998
更新日期:1998-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.47.4.886-887.1984
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4901-4907.2001
更新日期:2001-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.72.3.2014-2021.2006
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01421-07
更新日期:2008-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02360-12
更新日期:2013-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03999-14
更新日期:2015-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.8.4906-4910.2004
更新日期:2004-08-01 00:00:00