Dichloran-rose bengal medium for enumeration and isolation of molds from foods.

Abstract:

:Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.

journal_name

Appl Environ Microbiol

authors

King AD Jr,Hocking AD,Pitt JI

doi

10.1128/AEM.37.5.959-964.1979

subject

Has Abstract

pub_date

1979-05-01 00:00:00

pages

959-64

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

37

pub_type

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