Abstract:
:Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were assessed. Field plots of bush beans (Phaseolus vulgaris), sprayed with the bacterium Pseudomonas syringae, Pseudomonas fluorescens, or Erwinia herbicola, received the following treatments: (i) control, (ii) tillage, (iii) burning, (iv) burning plus tillage (burn-tillage), (v) Kocide (cupric hydroxide), (vi) Kocide plus tillage, (vii) Agri-Strep (streptomycin sulfate), and (viii) Agri-Strep plus tillage. Leaves and soil from the plots were sampled at 1 day before and at 1, 3, 7, 10, 14, 21, and 30 days after application of the decontamination treatments. The burn and burn-tillage treatments produced the most significant reductions in bacterial populations. The Agri-Strep treatment was more effective than the Kocide treatment in eliminating applied bacteria, but neither biocide produced consistent or persistent control. In contrast, the tillage treatment, alone or in combination with the Agri-Strep or Kocide treatments, had a short-term stimulatory effect and increased populations of applied bacteria and also levels of indigenous fungi and bacteria. Agri-Strep and Kocide treatments caused significant reductions in indigenous bacterial populations up to 14 days after application and in indigenous fungal populations on day 7 after application. Our results suggest that conventional plant disease control methods may not provide satisfactory control of genetically engineered microorganisms and indicate a need for further development of effective and selective methods to control release microorganisms at field sites.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Donegan K,Fieland V,Fowles N,Ganio L,Seidler Rdoi
10.1128/AEM.58.4.1207-1214.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1207-14issue
4eissn
0099-2240issn
1098-5336journal_volume
58pub_type
杂志文章abstract::The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.02072-07
更新日期:2008-06-01 00:00:00
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doi:10.1128/AEM.01797-10
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doi:10.1128/AEM.54.6.1399-1404.1988
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doi:10.1128/aem.66.6.2422-2429.2000
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.59.1.330-333.1993
更新日期:1993-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.10.3360-3366.1992
更新日期:1992-10-01 00:00:00
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doi:10.1128/aem.69.2.1067-1074.2003
更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.65.1.198-205.1999
更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.65.1.61-66.1999
更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.02567-08
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doi:10.1128/aem.67.9.3873-3881.2001
更新日期:2001-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.5.1234-1235.1983
更新日期:1983-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.4.697-700.1980
更新日期:1980-10-01 00:00:00
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doi:10.1128/AEM.61.11.3884-3888.1995
更新日期:1995-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.43.1.110-115.1982
更新日期:1982-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.8.3053-3056.1996
更新日期:1996-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.5.1078-1080.1980
更新日期:1980-05-01 00:00:00
abstract::Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunizatio...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.1.169-176.1982
更新日期:1982-01-01 00:00:00
abstract::Application of a mycorrhizal inoculum could be one way to increase the yield of rice plants and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading, and ripening stages in four paddy w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03769-14
更新日期:2015-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.11.5084-5093.2001
更新日期:2001-11-01 00:00:00
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pub_type: 杂志文章
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