Acetylene reduction assay for nitrogenase activity: gas chromatographic determination of ethylene per sample in less than one minute.

abstract：:Citrate metabolism by Enterococcus faecium FAIR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Ghijsels V,Tsakalidou E,De Vuyst L

更新日期：2006-01-01 00:00:00

abstract：:Total extracted DNA from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. This was done by PCR amplification and cloning of a DNA segment encoding the active site of the enzyme. The translated sequences obtained fell into three similarity clu...

journal_title：Applied and environmental microbiology

authors： Standfuss-Gabisch C,Al-Halbouni D,Hofer B

更新日期：2012-04-01 00:00:00

abstract：:Both the wild type and an isogenic hydrogenase-negative mutant of Azorhizobium caulinodans growing ex planta on N(2) as the N source were studied in succinate-limited steady-state chemostat cultures under 0.2 to 3.0% dissolved O(2) tension. Production or consumption of O(2), H(2), and CO(2) was measured with an on-lin...

journal_title：Applied and environmental microbiology

authors： Boogerd FC,Ferdinandy-van Vlerken MM,Mawadza C,Pronk AF,Stouthamer AH,van Verseveld HW

更新日期：1994-06-01 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：:beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

journal_title：Applied and environmental microbiology

authors： Rao MV,Dutta SM

更新日期：1977-08-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:Listeria monocytogenes secretes two chitinases and one chitin binding protein. Mutants lacking chiA, chiB, or lmo2467 exhibited normal growth in cultured cells but were defective for growth in the livers and spleens of mice. Mammals lack chitin; thus, L. monocytogenes may have adapted chitinases to recognize alternati...

journal_title：Applied and environmental microbiology

authors： Chaudhuri S,Bruno JC,Alonzo F 3rd,Xayarath B,Cianciotto NP,Freitag NE

更新日期：2010-11-01 00:00:00

abstract：:A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and...

journal_title：Applied and environmental microbiology

authors： Aurell H,Catala P,Farge P,Wallet F,Le Brun M,Helbig JH,Jarraud S,Lebaron P

更新日期：2004-03-01 00:00:00

abstract：:Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individual...

journal_title：Applied and environmental microbiology

authors： Jorgensen F,Ellis-Iversen J,Rushton S,Bull SA,Harris SA,Bryan SJ,Gonzalez A,Humphrey TJ

更新日期：2011-06-01 00:00:00

abstract：:Although many Lactobacillus strains used as probiotics are believed to modulate host immune responses, the molecular natures of the components of such probiotic microorganisms directly involved in immune modulation process are largely unknown. We aimed to assess the function of polysaccharide moiety of the cell wall o...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Serata M,Sako T

更新日期：2008-08-01 00:00:00

abstract：:Petuely's selective medium for Bifidobacterium was improved by addition of riboflavin, nucleic acid bases, pyruvic acid, and nalidixic acid. The modified medium, when examined under strictly anaerobic conditions for efficient isolation of Bifidobacterium from human fecal samples, exhibited selective and high viable co...

journal_title：Applied and environmental microbiology

authors： Tanaka R,Mutai M

更新日期：1980-11-01 00:00:00

abstract：:Gordonia polyisoprenivorans strain VH2, a potent rubber-degrading actinomycete, harbors two latex clearing proteins (Lcps), which are known to be essential for the microbial degradation of rubber. However, biochemical information on the exact role of this protein in the degradation of polyisoprene was lacking. In this...

journal_title：Applied and environmental microbiology

authors： Hiessl S,Böse D,Oetermann S,Eggers J,Pietruszka J,Steinbüchel A

更新日期：2014-09-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...

journal_title：Applied and environmental microbiology

authors： Erdman MD,Bergen WG,Reddy CA

更新日期：1977-04-01 00:00:00

abstract：:Several Bacillus and Paenibacillus species were isolated from Fe and Mn oxide minerals precipitating at a deep subsurface oxic-anoxic interface at Henderson Molybdenum Mine, Empire, CO. The isolates were investigated for their Mn(II)-oxidizing potential and interrogated for possession of the mnxG gene, a gene that cod...

journal_title：Applied and environmental microbiology

authors： Mayhew LE,Swanner ED,Martin AP,Templeton AS

更新日期：2008-12-01 00:00:00

abstract：:A rapid coliphage analysis technique for enumerating coliphages in natural waters has been evaluated by water quality laboratories located throughout the United States. Correlations were established between coliphages and coliforms in natural water systems. These correlations were highly significant. This relationship...

journal_title：Applied and environmental microbiology

authors： Wentsel RS,O'Neill PE,Kitchens JF

更新日期：1982-02-01 00:00:00

abstract：:The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect f...

journal_title：Applied and environmental microbiology

authors： Busta FF,Baillie E,Murrell WG

更新日期：1976-08-01 00:00:00

abstract：:The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of signific...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Gaskell J,Mozuch M,Kersten P,Sabat G,Martinez D,Cullen D

更新日期：2009-06-01 00:00:00

abstract：:The frequency of recovery of atypical mycobacteria was estimated in two treatment plants providing drinking water to Paris, France, at some intermediate stages of treatment. The two plants use two different filtration processes, rapid and slow sand filtration. Our results suggest that slow sand filtration is more effi...

journal_title：Applied and environmental microbiology

authors： Le Dantec C,Duguet JP,Montiel A,Dumoutier N,Dubrou S,Vincent V

更新日期：2002-11-01 00:00:00

abstract：:POL-88, a mutant of the white-rot fungus Phanerochaete chrysosporium, was selected for diminished phenol-oxidizing enzyme activity. A wide variety of phenolic compounds were degraded by ligninolytic cultures of this mutant. With several o-diphenolic substrates, degradation intermediates were produced that had UV spect...

journal_title：Applied and environmental microbiology

authors： Leatham GF,Crawford RL,Kirk TK

更新日期：1983-07-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne pathogen with the ability to grow under conditions of high osmolarity. In a previous study, we reported the identification of 12 proteins showing high induction after salt stress. One of these proteins is highly similar to the general stress protein Ctc of Bacillus subtilis. In t...

journal_title：Applied and environmental microbiology

authors： Gardan R,Duché O,Leroy-Sétrin S,Labadie J,European Listeria Genome Consortium.

更新日期：2003-01-01 00:00:00

abstract：:Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, "Natronoanaerobium," and Clostridium. Two novel therm...

journal_title：Applied and environmental microbiology

authors： Kaksonen AH,Plumb JJ,Robertson WJ,Spring S,Schumann P,Franzmann PD,Puhakka JA

更新日期：2006-05-01 00:00:00

abstract：:Pot experiments showed that Rhizobium japonicum cells entrapped in a polyacrylamide gel could be used as an inoculant for soybeans and compared favorably to laboratory-made peat base inoculant containing the same bacterial strain. ...

journal_title：Applied and environmental microbiology

authors： Dommergues YR,Diem HG,Divies C

更新日期：1979-04-01 00:00:00

abstract：:Salmonella is a zoonotic pathogen that poses a considerable public health and economic burden in the United States and worldwide. Resultant human diseases range from enterocolitis to bacteremia to sepsis and are acutely dependent on the particular serovar of Salmonella enterica subsp. enterica, which comprises over 99...

journal_title：Applied and environmental microbiology

authors： Patterson AS,Heithoff DM,Ferguson BS,Soh HT,Mahan MJ,Plaxco KW

更新日期：2013-04-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:Listeria monocytogenes is a foodborne pathogen that causes high rates of hospitalization and mortality in people infected. Contamination of fresh, ready to eat produce by this pathogen is especially troubling because of the ability of this bacterium to grow on produce under refrigeration temperatures. In this study, w...

journal_title：Applied and environmental microbiology

authors： Tran TD,Del Cid C,Hnasko R,Gorski L,McGarvey JA

更新日期：2020-12-17 00:00:00

abstract：:The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), res...

journal_title：Applied and environmental microbiology

authors： Glenn AE,Hinton DM,Yates IE,Bacon CW

更新日期：2001-07-01 00:00:00