Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry.

Abstract:

:A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and 12 to 15 in tap water samples within 3 to 4 h. The sensitivity of the method was between 10 and 100 bacteria per liter and was principally limited by the filtration capacity of membranes. The specificity of the antibody was evaluated against 15 non-Legionella strains, and no cross-reactivity was observed. When the method was applied to natural waters, direct counts of L. pneumophila were compared with the number of CFU obtained by the standard culture method. Direct counts were always higher than culturable counts, and the ratio between the two methods ranged from 1.4 to 325. Solid-phase cytometry offers a fast and sensitive alternative to the culture method for L. pneumophila screening in hot water systems.

journal_name

Appl Environ Microbiol

authors

Aurell H,Catala P,Farge P,Wallet F,Le Brun M,Helbig JH,Jarraud S,Lebaron P

doi

10.1128/aem.70.3.1651-1657.2004

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

1651-7

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

70

pub_type

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