Production of lycopene in the non-carotenoid-producing yeast Yarrowia lipolytica.

abstract：:A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

journal_title：Applied and environmental microbiology

authors： Bouhenni R,Gehrke A,Saffarini D

更新日期：2005-08-01 00:00:00

abstract：:Escherichia coli KO11 was previously constructed to produce ethanol from acid hydrolysates of hemicellulose (pentoses and hexoses) by the chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB). Klebsiella oxytoca P2 was constructed in an analogous fash...

journal_title：Applied and environmental microbiology

authors： Moniruzzaman M,Lai X,York SW,Ingram LO

更新日期：1997-12-01 00:00:00

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

journal_title：Applied and environmental microbiology

authors： Smith RA,Couche GA

更新日期：1991-01-01 00:00:00

abstract：:Prodigiosin (PG), a red linear tripyrrole pigment normally secreted by Serratia marcescens, has received attention for its reported immunosuppressive, antimicrobial, and anticancer properties. Although several genes have been shown to be important for prodigiosin synthesis, information on the regulatory mechanisms beh...

journal_title：Applied and environmental microbiology

authors： Pan X,Tang M,You J,Liu F,Sun C,Osire T,Fu W,Yi G,Yang T,Yang ST,Rao Z

更新日期：2021-01-04 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:The percentage of azygospores of Gigaspora margarita with zoosporangia of chytridiaceous fungi (CF) was reduced significantly after agitating them in fenaminosulf before incubation in soil. Fenaminosulf did not affect zoosporangia development on chlamydospores of Glomus fasciculatum. Metalaxyl and ethazol were not eff...

journal_title：Applied and environmental microbiology

authors： Sylvia DM,Schenck NC

更新日期：1983-04-01 00:00:00

abstract：:To define conditions promoting inherited infection by Lyme disease spirochetes in Ixodes ticks, we variously infected ticks with Borrelia afzelii and examined their progenies by dark-field microscopy, immunofluorescence, PCR, and serial passage. No episode of inherited infection was evident, regardless of instar or ge...

journal_title：Applied and environmental microbiology

authors： Matuschka FR,Schinkel TW,Klug B,Spielman A,Richter D

更新日期：1998-08-01 00:00:00

abstract：:The molecular epidemiology of 545 Salmonella enterica serovar Typhimurium isolates collected between 1977 and 2009 from cattle in Hokkaido, Japan, was investigated using pulsed-field gel electrophoresis (PFGE). Nine main clusters were identified from 116 PFGE patterns. Cluster I comprised 248 isolates, 243 of which po...

journal_title：Applied and environmental microbiology

authors： Tamamura Y,Uchida I,Tanaka K,Okazaki H,Tezuka S,Hanyu H,Kataoka N,Makino S,Kishima M,Kubota T,Kanno T,Hatama S,Ishihara R,Hata E,Yamada H,Nakaoka Y,Akiba M

更新日期：2011-03-01 00:00:00

abstract：:The structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type I propagation by using singly nine strains of Lactobacillus sanfranciscensis. Under back-slopping propagation with wheat flour type 0 F114, cell numbers of presumptive lactic acid bacteria varie...

journal_title：Applied and environmental microbiology

authors： Siragusa S,Di Cagno R,Ercolini D,Minervini F,Gobbetti M,De Angelis M

更新日期：2009-02-01 00:00:00

abstract：:Five species of the genus Dunaliella (D. tertiolecta, D. primolecta, D. parva, D. bardawil, and D. salina) were examined for glycerol accumulation, growth rate, cell density, and protein and chlorophyll content. The suitability of each algal species for use as a fermentation substrate was judged according to glycerol ...

journal_title：Applied and environmental microbiology

authors： Nakas JP,Schaedle M,Parkinson CM,Coonley CE,Tanenbaum SW

更新日期：1983-11-01 00:00:00

abstract：:Cytochrome P450 BM-3, a self-sufficient P450 enzyme from Bacillus megaterium that catalyzes the subterminal hydroxylation of long-chain fatty acids, has been engineered into a catalyst for the oxidation of polycyclic aromatic hydrocarbons. The activities of a triplet mutant (A74G/F87V/L188Q) towards naphthalene, fluor...

journal_title：Applied and environmental microbiology

authors： Li QS,Ogawa J,Schmid RD,Shimizu S

更新日期：2001-12-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:The effect of yeast hulls (yeast ghosts) on sluggish or stuck white wine fermentations was studied. The enhancing effect on yeast growth and fermentation rate displayed by the hulls was shown to be similar to the effect provided by lipid extract from the same hulls. Unsaturated fatty acids and sterols were incorporate...

journal_title：Applied and environmental microbiology

authors： Munoz E,Ingledew WM

更新日期：1989-06-01 00:00:00

abstract：:Intracellular pH (pH(in)) is a tightly regulated physiological parameter, which controls cell performance in all living systems. The purpose of this work was to evaluate if and how H(+) homeostasis is accomplished by an industrial wine strain of Saccharomyces cerevisiae while fermenting real must under the harsh winer...

journal_title：Applied and environmental microbiology

authors： Viana T,Loureiro-Dias MC,Loureiro V,Prista C

更新日期：2012-09-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:phi-0303 is a temperate bacteriophage isolated from Lactobacillus helveticus CNRZ 303 strain after mitomycin C induction. In this work, the gene coding for a lytic protein of this bacteriophage was cloned using a library of phi-0303 in Escherichia coli DH5alpha. The lytic activity was detected by its expression, using...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Guezenec S,Piot M,Foster S,Lortal S

更新日期：2004-01-01 00:00:00

abstract：:Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

journal_title：Applied and environmental microbiology

authors： Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

更新日期：2012-01-01 00:00:00

abstract：:Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. S...

journal_title：Applied and environmental microbiology

authors： Lin JL,Joye SB,Scholten JC,Schäfer H,McDonald IR,Murrell JC

更新日期：2005-10-01 00:00:00

abstract：:Pyrobaculum islandicum uses iron, thiosulfate, and elemental sulfur for anaerobic respiration, while Pyrobaculum aerophilum uses iron and nitrate; however, the constraints on these processes and their physiological mechanisms for iron and sulfur reduction are not well understood. Growth rates on sulfur compounds are h...

journal_title：Applied and environmental microbiology

authors： Feinberg LF,Srikanth R,Vachet RW,Holden JF

更新日期：2008-01-01 00:00:00

abstract：:The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

journal_title：Applied and environmental microbiology

authors： Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

更新日期：2007-05-01 00:00:00

abstract：:A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

journal_title：Applied and environmental microbiology

authors： Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

更新日期：2014-12-01 00:00:00

abstract：:Resting cells of Micrococcus luteus have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl(2) at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not re...

journal_title：Applied and environmental microbiology

authors： Faison BD,Cancel CA,Lewis SN,Adler HI

更新日期：1990-12-01 00:00:00

abstract：:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

journal_title：Applied and environmental microbiology

authors： Sembries S,Crawford RL

更新日期：1997-05-01 00:00:00

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The v...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Fouz B,Alcaide E,Amaro C

更新日期：1996-03-01 00:00:00

abstract：:pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Salyers AA

更新日期：1985-10-01 00:00:00

abstract：:Although research has increasingly focused on the pathogenesis of avian pathogenic Escherichia coli (APEC) infections and the "APEC pathotype" itself, little is known about the reservoirs of these bacteria. We therefore compared outbreak strains isolated from diseased chickens (n = 121) with nonoutbreak strains, inclu...

journal_title：Applied and environmental microbiology

authors： Ewers C,Antão EM,Diehl I,Philipp HC,Wieler LH

更新日期：2009-01-01 00:00:00

abstract：:n-Alkanes are ubiquitous in nature and are widely used by microorganisms as carbon sources. Alkane hydroxylation by alkane monooxygenases is a critical step in the aerobic biodegradation of n-alkanes, which plays important roles in natural alkane attenuation and is used in industrial and environmental applications. Th...

journal_title：Applied and environmental microbiology

authors： Liang JL,Gao Y,He Z,Nie Y,Wang M,JiangYang JH,Zhang XC,Shu WS,Wu XL

更新日期：2017-10-17 00:00:00