Competition for light between toxic and nontoxic strains of the harmful cyanobacterium Microcystis.

abstract：:Most Shiga toxin-producing Escherichia coli (STEC) strains associated with severe disease, such as hemolytic-uremic syndrome (HUS), carry large enterohemolysin-encoding (ehxA) plasmids, e.g., pO157 and pO103, that contribute to STEC clinical manifestations. Six ehxA subtypes (A through F) exist that phylogenetically c...

journal_title：Applied and environmental microbiology

authors： Lorenz SC,Monday SR,Hoffmann M,Fischer M,Kase JA

更新日期：2016-10-14 00:00:00

abstract：:Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This meth...

journal_title：Applied and environmental microbiology

authors： Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom ME

更新日期：2006-02-01 00:00:00

abstract：:With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...

journal_title：Applied and environmental microbiology

authors： Restaino L,Myron JJ,Lenovich LM,Bills S,Tscherneff K

更新日期：1984-04-01 00:00:00

abstract：:Soil samples were screened to select microorganisms with the capability to oxidize organic sulfides into the corresponding sulfoxides with differential enantioselectivities. Several bacterial strains that preferentially produced the S-configured sulfoxide enantiomer were isolated. Surprisingly, one bacterial strain, g...

journal_title：Applied and environmental microbiology

authors： Adam W,Heckel F,Saha-Möller CR,Taupp M,Meyer JM,Schreier P

更新日期：2005-04-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:Enterotoxin B produced by Staphylococus aureus 243 in brain heart infusion broth was concentrated by dialysis against 40% polyethylene glycol (20 M), partially purified on a Sephadex G-100 column and heated at 110 degrees C in thermal death time cans. Various heating menstrua included 0.04 M Veronal buffer (pH 7.4), b...

journal_title：Applied and environmental microbiology

authors： Lee IC,Stevenson KE,Harmon LG

更新日期：1977-02-01 00:00:00

abstract：:During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the (13)C-pigment molecules from mycelia cultivated with [1-(13)C]-, [2-(13)C]-...

journal_title：Applied and environmental microbiology

authors： Hajjaj H,Klaébé A,Goma G,Blanc PJ,Barbier E,François J

更新日期：2000-03-01 00:00:00

abstract：:The effects of aflatoxin on egg production, fertility, hatchability, and progeny performance were investigated by feeding dietary aflatoxin at dose levels of 0,5, and 10 mug/g to mature broiler breeder hens for 4 weeks. Sixteen hens were used for each dietary dose level. Egg production decreased significantly during w...

journal_title：Applied and environmental microbiology

authors： Howarth B Jr,Wyatt RD

更新日期：1976-05-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:This work reports the utilization of an in vivo expression technology system to identify in vivo-induced (ivi) genes in Yersinia ruckeri after determination of the conditions needed for its selection in fish. Fourteen clones were selected, and the cloned DNA fragments were analyzed after partial sequencing. In additio...

journal_title：Applied and environmental microbiology

authors： Fernández L,Márquez I,Guijarro JA

更新日期：2004-09-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

journal_title：Applied and environmental microbiology

authors： Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

更新日期：2017-08-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% NaCl were monitored by single-...

journal_title：Applied and environmental microbiology

authors： Kleinsteuber S,Riis V,Fetzer I,Harms H,Müller S

更新日期：2006-05-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

journal_title：Applied and environmental microbiology

authors： Jin YS,Alper H,Yang YT,Stephanopoulos G

更新日期：2005-12-01 00:00:00

abstract：:Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

journal_title：Applied and environmental microbiology

authors： Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

更新日期：2006-04-01 00:00:00

abstract：:In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the...

journal_title：Applied and environmental microbiology

authors： Pélandakis M,De Jonckheere JF,Pernin P

更新日期：1998-08-01 00:00:00

abstract：:The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

journal_title：Applied and environmental microbiology

authors： Marquis RE,Bender GR,Murray DR,Wong A

更新日期：1987-01-01 00:00:00

abstract：:Long periods of experimental incubation with acetylene led to a multifold enhancement of acetylene-reducing activity in Anabaena cylindrica, Anabaenopsis circularis, Rhodospirillum rubrum, and Azotobacter vinelandii. Rates of acetylene reduction showed a gradual increase and reached a peak after 2 to 6 h of continuous...

journal_title：Applied and environmental microbiology

authors： David KA,Fay P

更新日期：1977-12-01 00:00:00

abstract：:Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

journal_title：Applied and environmental microbiology

authors： Ahonen L,Tuovinen OH

更新日期：1990-02-01 00:00:00

abstract：:The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection ma...

journal_title：Applied and environmental microbiology

authors： Posno M,Heuvelmans PT,van Giezen MJ,Lokman BC,Leer RJ,Pouwels PH

更新日期：1991-09-01 00:00:00

abstract：:Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

journal_title：Applied and environmental microbiology

authors： Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

更新日期：2013-09-01 00:00:00

abstract：:The microbial degradation of tensile test pieces made of poly(3-hydroxybutyrate) [P(3HB)] or a copolymer of 90% 3-hydroxybutyric acid and 10% 3-hydroxyvaleric acid was studied in soils incubated at a constant temperature of 15, 28, or 40 degrees C for up to 200 days. In addition, hydrolytic degradation in sterile buff...

journal_title：Applied and environmental microbiology

authors： Mergaert J,Webb A,Anderson C,Wouters A,Swings J

更新日期：1993-10-01 00:00:00

abstract：:The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

journal_title：Applied and environmental microbiology

authors： Desomer J,Dhaese P,Montagu MV

更新日期：1990-09-01 00:00:00

abstract：:The potential for removing uranium from contaminated groundwater by stimulating the in situ activity of dissimilatory metal-reducing microorganisms was evaluated in a uranium-contaminated aquifer located in Rifle, Colo. Acetate (1 to 3 mM) was injected into the subsurface over a 3-month period via an injection gallery...

journal_title：Applied and environmental microbiology

authors： Anderson RT,Vrionis HA,Ortiz-Bernad I,Resch CT,Long PE,Dayvault R,Karp K,Marutzky S,Metzler DR,Peacock A,White DC,Lowe M,Lovley DR

更新日期：2003-10-01 00:00:00

abstract：:The ability of terminal restriction fragment (T-RFLP or TRF) profiles of 16S rRNA genes to provide useful information about the relative diversity of complex microbial communities was investigated by comparison with other methods. Four soil communities representing two pinyon rhizosphere and two between-tree (interspa...

journal_title：Applied and environmental microbiology

authors： Dunbar J,Ticknor LO,Kuske CR

更新日期：2000-07-01 00:00:00

abstract：:A Comamonas testosteroni strain was isolated from activated sludge on the basis of its ability to coaggregate with yeast cells. On agar plates the following two types of colonies were formed: colonies with a mucoid appearance and colonies with a nonmucoid appearance. On plates this strain alternated between the two fo...

journal_title：Applied and environmental microbiology

authors： Bossier P,Verstraete W

更新日期：1996-08-01 00:00:00

abstract：:We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. col...

journal_title：Applied and environmental microbiology

authors： Salinger N,Kokona B,Fairman R,Okeke IN

更新日期：2009-01-01 00:00:00