Genetic variation in the free-living amoeba Naegleria fowleri.

Abstract:

:In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the variant previously detected in Asia, Europe, and the United States. In France, surprisingly, strains from Cattenom gave RAPD patterns identical to those of the Japanese strains. In addition, all of these strains, together with an additional French strain from Chooz, exhibited similarities to South Pacific strains. The results also confirmed the presence of numerous variants in Europe, whereas only two variants were detected in the United States. The two variants found in the United States were different from the South Pacific variants. These findings do not support the previous hypothesis concerning the origin and modes of dispersal of N. fowleri.

journal_name

Appl Environ Microbiol

authors

Pélandakis M,De Jonckheere JF,Pernin P

doi

10.1128/AEM.64.8.2977-2981.1998

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

2977-81

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

杂志文章
  • Cometabolic degradation of dibenzofuran by biphenyl-cultivated Ralstonia sp. strain SBUG 290.

    abstract::Cells of the gram-negative bacterium Ralstonia sp. strain SBUG 290 grown in the presence of biphenyl are able to cooxidize dibenzofuran which has been 1,2-hydroxylated. Meta cleavage of the 1, 2-dihydroxydibenzofuran between carbon atoms 1 and 9b produced 2-hydroxy-4-(3'-oxo-3'H-benzofuran-2'-yliden)but-2-enoic acid, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4528-4531.2000

    authors: Becher D,Specht M,Hammer E,Francke W,Schauer F

    更新日期:2000-10-01 00:00:00

  • Appearance of enzyme activities catalyzing conversion of sterigmatocystin to aflatoxin B1 in late-growth-phase Aspergillus parasiticus cultures.

    abstract::Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.7.1711-1713.1987

    authors: Cleveland TE,Lax AR,Lee LS,Bhatnagar D

    更新日期:1987-07-01 00:00:00

  • Role of exopolysaccharides in Pseudomonas aeruginosa biofilm formation and architecture.

    abstract::Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00637-11

    authors: Ghafoor A,Hay ID,Rehm BH

    更新日期:2011-08-01 00:00:00

  • Effect of aerosolization on culturability and viability of gram-negative bacteria.

    abstract::Estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. However, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3585-3588.1997

    authors: Heidelberg JF,Shahamat M,Levin M,Rahman I,Stelma G,Grim C,Colwell RR

    更新日期:1997-09-01 00:00:00

  • 2CS-CHXT Operon Signature of Chlorhexidine Tolerance among Enterococcus faecium Isolates.

    abstract::Chlorhexidine (CHX) is a broad-spectrum antiseptic widely used in community and clinical contexts for many years that has recently acquired higher relevance in nosocomial infection control worldwide. Despite this, CHX tolerance among Enterococcus faecium bacteria, representing one of the leading agents causing nosocom...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01589-19

    authors: Duarte B,Pereira AP,Freitas AR,Coque TM,Hammerum AM,Hasman H,Antunes P,Peixe L,Novais C

    更新日期:2019-11-14 00:00:00

  • Effects of pulsed electric fields on inactivation kinetics of Listeria innocua.

    abstract::The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5364-5371.1999

    authors: Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

    更新日期:1999-12-01 00:00:00

  • Deletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity.

    abstract::Aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01406-13

    authors: Hunter AJ,Morris TA,Jin B,Saint CP,Kelly JM

    更新日期:2013-09-01 00:00:00

  • Fermentative Degradation of Polyethylene Glycol by a Strictly Anaerobic, Gram-Negative, Nonsporeforming Bacterium, Pelobacter venetianus sp. nov.

    abstract::[This corrects the article on p. 1913 in vol. 45.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.2.532-532.1983

    authors:

    更新日期:1983-08-01 00:00:00

  • Effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by Enterobacter aerogenes.

    abstract::Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. Newly isolated Enterobacter aerogenes strain AJ110637, a rapid carbon source assimilator under weakly acidic (pH 5.0) conditions, was selected as a platform for succinate production. Our previo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03213-14

    authors: Tajima Y,Yamamoto Y,Fukui K,Nishio Y,Hashiguchi K,Usuda Y,Sode K

    更新日期:2015-02-01 00:00:00

  • Quantitative molecular analysis of the microbial community in marine arctic sediments (Svalbard).

    abstract::Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. U...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.387-395.2001

    authors: Ravenschlag K,Sahm K,Amann R

    更新日期:2001-01-01 00:00:00

  • Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization.

    abstract::Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04175-14

    authors: Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

    更新日期:2015-05-15 00:00:00

  • Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading variovorax strain.

    abstract::The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1532-1541.2003

    authors: Dejonghe W,Berteloot E,Goris J,Boon N,Crul K,Maertens S,Höfte M,De Vos P,Verstraete W,Top EM

    更新日期:2003-03-01 00:00:00

  • Dense autotrophic cultures of Alcaligenes eutrophus.

    abstract::Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.4.592-597.1976

    authors: Repaske R,Mayer R

    更新日期:1976-10-01 00:00:00

  • Monoclonal antibodies that detect live salmonellae.

    abstract::Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.12.3868-3872.1992

    authors: Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

    更新日期:1992-12-01 00:00:00

  • Selection of antibiotic-resistant standard plate count bacteria during water treatment.

    abstract::Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.308-316.1982

    authors: Armstrong JL,Calomiris JJ,Seidler RJ

    更新日期:1982-08-01 00:00:00

  • Management of Multiple Nitrogen Sources during Wine Fermentation by Saccharomyces cerevisiae.

    abstract::During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02617-16

    authors: Crépin L,Truong NM,Bloem A,Sanchez I,Dequin S,Camarasa C

    更新日期:2017-02-15 00:00:00

  • Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

    abstract::We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02630-07

    authors: Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

    更新日期:2008-05-01 00:00:00

  • Effects of rice seed surface sterilization with hypochlorite on inoculated Burkholderia vietnamiensis.

    abstract::When a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in CFU was observed during the first 15 h after inoculation of the rice rhizosphere organism Burkholderia vietnamiensis TVV75. This artifact could not be eliminated simply by rinsing the s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.3046-3052.2001

    authors: Miché L,Balandreau J

    更新日期:2001-07-01 00:00:00

  • Percolation and survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in soil amended with contaminated dairy manure or slurry.

    abstract::The effect of cattle manure and slurry application on percolation and survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium was investigated for different soil depths after the addition of water. Four treatments were chosen for the first set of experiments: (i) addition of inoculated farmyar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01791-08

    authors: Semenov AV,van Overbeek L,van Bruggen AH

    更新日期:2009-05-01 00:00:00

  • RedH and PigC Catalyze the Biosynthesis of Hybrubins via Phosphorylation of 4'-Methoxy-2,2'-Bipyrrole-5'-Carbaldehyde.

    abstract::Hybrubins are "unnatural" alkaloids with the same 4'-methoxy-2,2'-bipyrrole-5'-methine moiety found in prodiginines and a different ring derived from tetramic acids. Here, we demonstrated that RedH, a homologue of prodigiosin synthetase PigC, was responsible for the biosynthesis of hybrubins A and B in Streptomyces li...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02331-19

    authors: Long Q,Jeffries DE,Lin S,Chen X,He W,Wang Y,Deng Z,Lindsley CW,Tao M

    更新日期:2020-01-07 00:00:00

  • Bradyrhizobium japonicum Survival in and Soybean Inoculation with Fluid Gels.

    abstract::The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.3.617-622.1989

    authors: Jawson MD,Franzluebbers AJ,Berg RK

    更新日期:1989-03-01 00:00:00

  • Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability.

    abstract::Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00830-18

    authors: Geng M,Smith L

    更新日期:2018-07-17 00:00:00

  • Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.

    abstract::pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.958-964.1985

    authors: Kuritza AP,Salyers AA

    更新日期:1985-10-01 00:00:00

  • GELRITE as a Gelling Agent in Media for the Growth of Thermophilic Microorganisms.

    abstract::GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.2.427-429.1984

    authors: Lin CC,Casida LE

    更新日期:1984-02-01 00:00:00

  • Inter- and intraspecies plasmid-mediated transfer of florfenicol resistance in Enterobacteriaceae isolates from swine.

    abstract::Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02816-08

    authors: Rayamajhi N,Cha SB,Kang ML,Lee SI,Lee HS,Yoo HS

    更新日期:2009-09-01 00:00:00

  • Glucose triggers ATP secretion from bacteria in a growth-phase-dependent manner.

    abstract::ATP modulates immune cell functions, and ATP derived from gut commensal bacteria promotes the differentiation of T helper 17 (Th17) cells in the intestinal lamina propria. We recently reported that Enterococcus gallinarum, isolated from mice and humans, secretes ATP. We have since found and characterized several ATP-s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03871-12

    authors: Hironaka I,Iwase T,Sugimoto S,Okuda K,Tajima A,Yanaga K,Mizunoe Y

    更新日期:2013-04-01 00:00:00

  • Purification of Giardia muris cysts by velocity sedimentation.

    abstract::Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.2.454-455.1984

    authors: Sauch JF

    更新日期:1984-08-01 00:00:00

  • Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in Central Europe.

    abstract::To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6414-6419.2004

    authors: Richter D,Schlee DB,Allgöwer R,Matuschka FR

    更新日期:2004-11-01 00:00:00

  • A comparison of methods for counting viruses in aquatic systems.

    abstract::In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2283-2289.2000

    authors: Bettarel Y,Sime-Ngando T,Amblard C,Laveran H

    更新日期:2000-06-01 00:00:00

  • Protein degradation by ruminal microorganisms from sheep fed dietary supplements of urea, casein, or albumin.

    abstract::Ruminal fluid from sheep fed hay plus concentrate diets containing 1.8% urea, 6% casein, or 6% egg albumin had proteolytic activities of 4.12, 3.02, or 4.00 mg of [14C]casein hydrolyzed ml-1 h-1, respectively. Dietary albumin had no effect on the rate of albumin breakdown relative to that of casein (0.06). Greater num...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.4.751-753.1987

    authors: Wallace RJ,Broderick GA,Brammall ML

    更新日期:1987-04-01 00:00:00