Applications of a colorimetric plate assay for soluble methane monooxygenase activity.

Abstract:

:A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1-naphthol, which is formed when sMMO reacts with naphthalene, and o-dianisidine (tetrazotized). Methanotrophic colonies expressing sMMO turned deep purple when exposed successively to naphthalene and o-dianisidine. The method was evaluated within the contexts of two potential applications. The first was for the enumeration of Methylosinus trichosporium OB3b in a methane-amended, unsaturated soil column dedicated to vinyl chloride treatment. The second application was for the isolation and enumeration of sMMO-bearing methanotrophs from sanitary landfill soils. The technique was effective in both applications.

journal_name

Appl Environ Microbiol

authors

Graham DW,Korich DG,LeBlanc RP,Sinclair NA,Arnold RG

doi

10.1128/AEM.58.7.2231-2236.1992

keywords:

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

2231-6

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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