Cometabolism of citrate and glucose by Enterococcus faecium FAIR-E 198 in the absence of cellular growth.

Abstract:

:Citrate metabolism by Enterococcus faecium FAIR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a constant pH 8.0, no citrate was metabolized, although growth took place. The main end products of citrate metabolism were acetate, formate, acetoin, and carbon dioxide, whereas ethanol and diacetyl were present in smaller amounts. In the presence of glucose, citrate was cometabolized, but it did not contribute to growth. Also, more acetate and less acetoin were formed compared to growth in mMRS medium and in the absence of glucose. Most of the citrate was consumed during the stationary phase, indicating that energy generated by citrate metabolism was used for maintenance. Experiments with cell-free fermented mMRS medium indicated that E. faecium FAIR-E 198 was able to metabolize another energy source present in the medium.

journal_name

Appl Environ Microbiol

authors

Vaningelgem F,Ghijsels V,Tsakalidou E,De Vuyst L

doi

10.1128/AEM.72.1.319-326.2006

keywords:

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

319-26

issue

1

eissn

0099-2240

issn

1098-5336

pii

72/1/319

journal_volume

72

pub_type

杂志文章
  • Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.

    abstract::Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.493-497.1986

    authors: Desjardins AE,Plattner RD,Vanmiddlesworth F

    更新日期:1986-03-01 00:00:00

  • A virulent phage infecting Lactococcus garvieae, with homology to Lactococcus lactis phages.

    abstract::A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02603-15

    authors: Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

    更新日期:2015-12-01 00:00:00

  • Feral Swine in the United States Have Been Exposed to both Avian and Swine Influenza A Viruses.

    abstract::Influenza A viruses (IAVs) in swine can cause sporadic infections and pandemic outbreaks among humans, but how avian IAV emerges in swine is still unclear. Unlike domestic swine, feral swine are free ranging and have many opportunities for IAV exposure through contacts with various habitats and animals, including migr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01346-17

    authors: Martin BE,Sun H,Carrel M,Cunningham FL,Baroch JA,Hanson-Dorr KC,Young SG,Schmit B,Nolting JM,Yoon KJ,Lutman MW,Pedersen K,Lager K,Bowman AS,Slemons RD,Smith DR,DeLiberto T,Wan XF

    更新日期:2017-09-15 00:00:00

  • Antagonism of Lactic Acid Bacteria against Phytopathogenic Bacteria.

    abstract::A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.3.552-555.1986

    authors: Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

    更新日期:1986-09-01 00:00:00

  • Diverse virulence gene content of Shiga toxin-producing Escherichia coli from finishing swine.

    abstract::Shiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Curr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01761-14

    authors: Tseng M,Fratamico PM,Bagi L,Delannoy S,Fach P,Manning SD,Funk JA

    更新日期:2014-10-01 00:00:00

  • First evidence of lysogeny in Propionibacterium freudenreichii subsp. shermanii.

    abstract::Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.231-238.2001

    authors: Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

    更新日期:2001-01-01 00:00:00

  • Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126.

    abstract::In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01627-07

    authors: Schuler L,Ní Chadhain SM,Jouanneau Y,Meyer C,Zylstra GJ,Hols P,Agathos SN

    更新日期:2008-02-01 00:00:00

  • Kinetics of sulfur oxidation at suboptimal temperatures.

    abstract::Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.2.560-562.1990

    authors: Ahonen L,Tuovinen OH

    更新日期:1990-02-01 00:00:00

  • Solvent-Augmented Mineralization of Pyrene by a Mycobacterium sp.

    abstract::The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.7.2311-2316.1996

    authors: Jimenez IY,Bartha R

    更新日期:1996-07-01 00:00:00

  • Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.

    abstract::Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.4117-4120.2005

    authors: Fierer N,Jackson JA,Vilgalys R,Jackson RB

    更新日期:2005-07-01 00:00:00

  • Activity of glutaraldehyde at low concentrations (less than 2%) against poliovirus and its relevance to gastrointestinal endoscope disinfection procedures.

    abstract::The activity of glutaraldehyde (GTA) at low concentrations (less than 2%) against poliovirus was assessed by a suspension procedure. The inactivation kinetics showed that concentrations of less than or equal to 0.10% were effective against purified poliovirus at pH 7.2; a 1 log10 reduction was obtained in 70 min with ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.4.1156-1160.1991

    authors: Bailly JL,Chambon M,Peigue-Lafeuille H,Laveran H,De Champs C,Beytout D

    更新日期:1991-04-01 00:00:00

  • Two types of endosymbiotic bacteria in the enigmatic marine worm Xenoturbella bocki.

    abstract::Two types of endosymbiotic bacteria were identified in the gastrodermis of the marine invertebrate Xenoturbella bocki (Xenoturbellida, Bilateria). While previously described Chlamydia-like endosymbionts were rare, Gammaproteobacteria distantly related to other endosymbionts and pathogens were abundant. The endosymbion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01092-09

    authors: Kjeldsen KU,Obst M,Nakano H,Funch P,Schramm A

    更新日期:2010-04-01 00:00:00

  • Shiga toxins, and the genes encoding them, in fecal samples from native Idaho ungulates.

    abstract::Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle ( approximately 19%). ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01158-08

    authors: Gilbreath JJ,Shields MS,Smith RL,Farrell LD,Sheridan PP,Spiegel KM

    更新日期:2009-02-01 00:00:00

  • Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization.

    abstract::Microbiological, genomic and transcriptomic analyses were used to examine three species from the bacterial genus Caldicellulosiruptor with respect to their capacity to convert the carbohydrate content of lignocellulosic biomass at 70°C to simple sugars, acetate, lactate, CO2, and H2. Caldicellulosiruptor bescii, C. kr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01622-15

    authors: Zurawski JV,Conway JM,Lee LL,Simpson HJ,Izquierdo JA,Blumer-Schuette S,Nookaew I,Adams MW,Kelly RM

    更新日期:2015-10-01 00:00:00

  • Microbial populations involved in cycling of dimethyl sulfide and methanethiol in freshwater sediments.

    abstract::Although several microorganisms that produce and degrade methanethiol (MT) and dimethyl sulfide (DMS) have been isolated from various habitats, little is known about the numbers of these microorganisms in situ. This study reports on the identification and quantification of microorganisms involved in the cycling of MT ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1044-1051.2001

    authors: Lomans BP,Luderer R,Steenbakkers P,Pol A,van Der Drift C,Vogels GD,Op den Camp HJ

    更新日期:2001-03-01 00:00:00

  • Microplate assay for colletotrichum spore production.

    abstract::A simple microplate method was devised to assay spore production by Colletotrichum gloeosporioides by growing the fungus on 1 ml of solid media in the wells of tissue culture plates. Growth and sporulation on microplates were compared at days 4 and 8 with growth and sporulation in 100-ml liquid batch cultures that inv...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.4.627-632.1987

    authors: Slade SJ,Harris RF,Smith CS,Andrews JH,Nordheim EV

    更新日期:1987-04-01 00:00:00

  • Genome-wide transcriptional and physiological responses of Bradyrhizobium japonicum to paraquat-mediated oxidative stress.

    abstract::The rhizobial bacterium Bradyrhizobium japonicum functions as a nitrogen-fixing symbiont of the soybean plant (Glycine max). Plants are capable of producing an oxidative burst, a rapid proliferation of reactive oxygen species (ROS), as a defense mechanism against pathogenic and symbiotic bacteria. Therefore, B. japoni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00047-11

    authors: Donati AJ,Jeon JM,Sangurdekar D,So JS,Chang WS

    更新日期:2011-06-01 00:00:00

  • Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae.

    abstract::Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.3.373-378.1979

    authors: Hemelt DM,Mares B,Upadhyay JM

    更新日期:1979-09-01 00:00:00

  • Dissolved Microcystin Release Coincident with Lysis of a Bloom Dominated by Microcystis spp. in Western Lake Erie Attributed to a Novel Cyanophage.

    abstract::Western Lake Erie (Laurentian Great Lakes) is prone to annual cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis spp. that often yield microcystin toxin concentrations exceeding the federal EPA recreational contact advisory of 8 μg liter-1 In August 2014, microcystin levels were detected in finished ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01397-20

    authors: McKindles KM,Manes MA,DeMarco JR,McClure A,McKay RM,Davis TW,Bullerjahn GS

    更新日期:2020-10-28 00:00:00

  • Evaluation of a tangential-flow multiple-filter technique for detection of Giardia lamblia cysts in water.

    abstract::A system of tangential-flow filtration was evaluated for use in the detection of Giardia cysts in drinking water. This method was more sensitive in recovering cysts than a frequently used wound-orlon system of through-filtration. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.2.400-402.1986

    authors: Isaac-Renton JL,Fung CP,Lochan A

    更新日期:1986-08-01 00:00:00

  • Effects of long-term storage on plasmid stability in Bacillus anthracis.

    abstract::The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.7778-7780.2005

    authors: Marston CK,Hoffmaster AR,Wilson KE,Bragg SL,Plikaytis B,Brachman P,Johnson S,Kaufmann AF,Popovic T

    更新日期:2005-12-01 00:00:00

  • L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.

    abstract::Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.3.1869-1873.2004

    authors: van der Kaaij H,Desiere F,Mollet B,Germond JE

    更新日期:2004-03-01 00:00:00

  • Direct effects of UV-B radiation on the freshwater heterotrophic nanoflagellate Paraphysomonas sp.

    abstract::The formation of DNA photoproducts in organisms exposed to ambient levels of UV-B radiation can lead to death and/or reduced population growth in aquatic systems. Dependence on photoenzymatic repair to reverse DNA damage caused by UV-B radiation is demonstrated for Paraphysomonas sp., a member of a widely distributed ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02803-08

    authors: Macaluso AL,Mitchell DL,Sanders RW

    更新日期:2009-07-01 00:00:00

  • Dead or alive: deformed wing virus and Varroa destructor reduce the life span of winter honeybees.

    abstract::Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06537-11

    authors: Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

    更新日期:2012-02-01 00:00:00

  • Nutritionally limited pectinolytic bacteria from the human intestine.

    abstract::A selective procedure was used to isolate pectinolytic intestinal bacteria from human subjects. The three isolates with the greatest pectinolytic activity utilized pectin and a few related compounds as fermentable substrates for growth but did not utilize any other compound tested. Thus, their substrate utilization pa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.1.172-173.1985

    authors: Jensen NS,Canale-Parola E

    更新日期:1985-07-01 00:00:00

  • Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities.

    abstract::Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2823-2828.2001

    authors: Thar R,Kühl M,Holst G

    更新日期:2001-06-01 00:00:00

  • Cell-Free Solubilization of Coal by Polyporus versicolor.

    abstract::Solubilization of coal was demonstrated with filtrates (0.45-mum-pore-size filters) obtained from the broth in which Polyporus versicolor had grown. The rate and extent of solubilization were dependent on the age of the fungal cultures, the particle size of the coal, the pH of the filtrates, and the presence of protei...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2840-2843.1987

    authors: Cohen MS,Bowers WC,Aronson H,Gray ET

    更新日期:1987-12-01 00:00:00

  • Characterization of an 8.7-kilobase thiostrepton resistance-encoding plasmid (pGIF3) of Streptomyces incarnatus.

    abstract::The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thios...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.3.895-899.1992

    authors: Malina H,Robert-Gero M

    更新日期:1992-03-01 00:00:00

  • Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile.

    abstract::The thermoacidophile Acidianus is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00334-18

    authors: Amenabar MJ,Boyd ES

    更新日期:2018-05-31 00:00:00

  • Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective

    abstract::The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.5020-5022.1998

    authors: Robleto EA,Borneman J,Triplett EW

    更新日期:1998-12-01 00:00:00