Abstract:
:Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fold purification was obtained. It was found that 5 and 10 mM MgCl2 significantly increased the bacteriolytic activity. It is a basic protein. The cell walls of Micrococcus lysodeikticus were lysed by the enzyme, and the products of digestion were purified by Amberlite CG-120 and Sephadex G-15 chromatography to facilitate the detection of amino sugars. After reduction of the oligosaccharides with sodium borohydride and acid hydrolysis, the amino sugars were identified by paper chromatography. It was found that enzyme II cleaved the glycosidic bond between N-acetylmuramic and and N-acetylglucosamine of the peptidoglycan moiety of the cell walls. Thus, the enzyme was identified as endo-beta-N-acetylmuramidase.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hemelt DM,Mares B,Upadhyay JMdoi
10.1128/AEM.38.3.373-378.1979subject
Has Abstractpub_date
1979-09-01 00:00:00pages
373-8issue
3eissn
0099-2240issn
1098-5336journal_volume
38pub_type
杂志文章abstract::The incorporation of tritiated thymidine by five microbial ecosystems and the distribution of tritium into DNA, RNA, and protein were determined. All microbial assemblages tested exhibited significant labeling of RNA and protein (i.e., nonspecific labeling), as determined by differential acid-base hydrolysis. Nonspeci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1245-1254.1990
更新日期:1990-05-01 00:00:00
abstract::Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater and seawater samples taken in Florida, the Gulf of Mexico, and the Bahamas Bank. Recoveries of T2 phage and calf thymus DNA added to artificial seawater and concentrated by VFF were 72.8 and 80%, respectively. Virus concentr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.8.2197-2204.1991
更新日期:1991-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.2.425-432.1989
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1400-1405.1990
更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.54.10.2492-2499.1988
更新日期:1988-10-01 00:00:00
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更新日期:2003-11-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-11-01 00:00:00
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更新日期:2015-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.31.1.11-20.1976
更新日期:1976-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.59.6.1767-1773.1993
更新日期:1993-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.1.15-18.1991
更新日期:1991-01-01 00:00:00
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doi:10.1128/AEM.36.4.589-593.1978
更新日期:1978-10-01 00:00:00
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doi:10.1128/AEM.54.3.753-759.1988
更新日期:1988-03-01 00:00:00
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更新日期:1992-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2717-2725.1990
更新日期:1990-09-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.63.7.2489-2494.1997
更新日期:1997-07-01 00:00:00
abstract::The fall armyworm, Spodoptera frugiperda, is an invasive maize pest that has spread from the Americas into Africa and Asia and causes severe crop damage worldwide. Most populations of S. frugiperda show low susceptibility to Bacillus thuringiensis (Bt) Cry1Ab or Cry1Ac toxins, which have been proved to be effective ag...
journal_title:Applied and environmental microbiology
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更新日期:2008-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.4.826-831.1983
更新日期:1983-10-01 00:00:00
abstract::Bacillus cereus UW85 produces a novel aminopolyol antibiotic, zwittermicin A, that contributes to the ability of UW85 to suppress damping-off of alfalfa caused by Phytophthora medicaginis. UW85 produces a second antibiotic, provisionally designated antibiotic B, which also contributes to suppression of damping-off but...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.12.4404-4412.1994
更新日期:1994-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.9.5004-5013.2005
更新日期:2005-09-01 00:00:00