Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments.

abstract：:A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

journal_title：Applied and environmental microbiology

authors： Ishige T,Tani A,Sakai Y,Kato N

更新日期：2000-08-01 00:00:00

abstract：:Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...

journal_title：Applied and environmental microbiology

authors： Williams JR,Morgan AG,Rouch DA,Brown NL,Lee BT

更新日期：1993-08-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:The ability of Listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. In order to understand the survival mechanisms of L. monocytogenes, an initial approach using two-dimensional polyacrylami...

journal_title：Applied and environmental microbiology

authors： Duché O,Trémoulet F,Glaser P,Labadie J

更新日期：2002-04-01 00:00:00

abstract：:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbati...

journal_title：Applied and environmental microbiology

authors： Macario AJ,Conway de Macario E,Ney U,Schoberth SM,Sahm H

更新日期：1989-08-01 00:00:00

abstract：:Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...

journal_title：Applied and environmental microbiology

authors： Lovett J,Francis DW,Hunt JM

更新日期：1983-08-01 00:00:00

abstract：:A betaproteobacterium within the family Rhodocyclaceae previously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs w...

journal_title：Applied and environmental microbiology

authors： Singleton DR,Hu J,Aitken MD

更新日期：2012-05-01 00:00:00

abstract：:Escherichia coli KO11 was previously constructed to produce ethanol from acid hydrolysates of hemicellulose (pentoses and hexoses) by the chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB). Klebsiella oxytoca P2 was constructed in an analogous fash...

journal_title：Applied and environmental microbiology

authors： Moniruzzaman M,Lai X,York SW,Ingram LO

更新日期：1997-12-01 00:00:00

abstract：:Intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. The quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microor...

journal_title：Applied and environmental microbiology

authors： Ercolini D,De Filippis F,La Storia A,Iacono M

更新日期：2012-11-01 00:00:00

abstract：:Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen Vibrio anguillarum. The aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a c...

journal_title：Applied and environmental microbiology

authors： Olsson JC,Westerdahl A,Conway PL,Kjelleberg S

更新日期：1992-02-01 00:00:00

abstract：:The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

journal_title：Applied and environmental microbiology

authors： Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

更新日期：2009-06-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, repetitive extragenic palindromic element (REP)-, and random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electr...

journal_title：Applied and environmental microbiology

authors： Hendson M,Purcell AH,Chen D,Smart C,Guilhabert M,Kirkpatrick B

更新日期：2001-02-01 00:00:00

abstract：:In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotran...

journal_title：Applied and environmental microbiology

authors： Yvon M,Chambellon E,Bolotin A,Roudot-Algaron F

更新日期：2000-02-01 00:00:00

abstract：:Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

journal_title：Applied and environmental microbiology

authors： Ng TK,Zeikus JG

更新日期：1986-10-01 00:00:00

abstract：:By using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the Aspergillus ochraceus group. A correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to...

journal_title：Applied and environmental microbiology

authors： Robbers JE,Hong S,Tuite J,Carlton WW

更新日期：1978-12-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:The incidence of microbes in the nasal cavities of workers in three paper and board mills was investigated. A total of 234 persons exposed to microbial aerosols and splashes from paper machine wires and debarker drums formed the exposed group. The control group consisted of 294 workers from the dry working areas: the ...

journal_title：Applied and environmental microbiology

authors： Niemelä SI,Väätänen P,Mentu J,Jokinen A,Jäppinen P,Sillanpää P

更新日期：1985-07-01 00:00:00

abstract：:Seven Azospirillum strains induced more deformation of root hairs of wheat than did strains of Rhizobium leguminosarum, Azotobacter chroococcum, or Escherichia coli. Azospirillum sp. strain Sp245 caused the most deformation. Strain Sp245 (isolated from surface sterile roots of wheat) and strain Sp7 (isolated from the ...

journal_title：Applied and environmental microbiology

authors： Jain DK,Patriquin DG

更新日期：1984-12-01 00:00:00

abstract：:Sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. Based on their hydrolysis profile patterns, Lactobacillus alimentarius 15M, Lactobacillus brevis 14G, Lactobacillus sanfranciscensis 7A, and Lactobacillus hilgardii...

journal_title：Applied and environmental microbiology

authors： Di Cagno R,De Angelis M,Lavermicocca P,De Vincenzi M,Giovannini C,Faccia M,Gobbetti M

更新日期：2002-02-01 00:00:00

abstract：:During growth on xylan and xylose Thermoanaerobacterium saccharolyticum B6A-RI produced endoxylanase, beta-xylosidase, arabinofuranosidase, and acetyl esterase, and the first three activities appeared to be produced coordinately. During nonlimiting growth on xylan, these enzyme activities were predominantly cell assoc...

journal_title：Applied and environmental microbiology

authors： Lee YE,Lowe SE,Zeikus JG

更新日期：1993-03-01 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individual...

journal_title：Applied and environmental microbiology

authors： Jorgensen F,Ellis-Iversen J,Rushton S,Bull SA,Harris SA,Bryan SJ,Gonzalez A,Humphrey TJ

更新日期：2011-06-01 00:00:00

abstract：:Erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due to E. rhusiopathiae serovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34 E. rhu...

journal_title：Applied and environmental microbiology

authors： Ogawa Y,Shiraiwa K,Ogura Y,Ooka T,Nishikawa S,Eguchi M,Hayashi T,Shimoji Y

更新日期：2017-05-17 00:00:00

abstract：:In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

journal_title：Applied and environmental microbiology

authors： Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

更新日期：2008-04-01 00:00:00

abstract：:The growth kinetics of Flavobacterium sp. strain S12 specialized in the utilization of glycerol, and a number of oligo- and polysaccharides were determined in batch-culture experiments at 15 degrees C in pasteurized tap water supplied with very low amounts of substrates. Kss for the growth on maltotriose, maltotetraos...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Hijnen WA

更新日期：1985-04-01 00:00:00

abstract：:The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize ...

journal_title：Applied and environmental microbiology

authors： Sabaratnam S,Beattie GA

更新日期：2003-02-01 00:00:00

abstract：:A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is det...

journal_title：Applied and environmental microbiology

authors： Hirsh DC,Martin LD

更新日期：1983-01-01 00:00:00