Salt stress proteins induced in Listeria monocytogenes.

Abstract:

:The ability of Listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. In order to understand the survival mechanisms of L. monocytogenes, an initial approach using two-dimensional polyacrylamide gel electrophoresis was performed to analyze the pattern of protein synthesis in response to salt stress. Of 400 to 500 visible proteins, the synthesis of 40 proteins (P < 0.05) was repressed or induced at a higher rate during salt stress. Some of the proteins were identified on the basis of mass spectrometry or N-terminal sequence analysis and database searching. Twelve proteins showing high induction after salt stress were similar to general stress proteins (Ctc and DnaK), transporters (GbuA and mannose-specific phosphotransferase system enzyme IIAB), and general metabolism proteins (alanine dehydrogenase, CcpA, CysK, EF-Tu, Gap, GuaB, PdhA, and PdhD).

journal_name

Appl Environ Microbiol

authors

Duché O,Trémoulet F,Glaser P,Labadie J

doi

10.1128/aem.68.4.1491-1498.2002

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

1491-8

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

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