Abstract:
:The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryptic soy agar slants overlaid with mineral oil. When data were stratified by decade, there was a decreasing linear trend in the proportion of strains containing both plasmids with increased storage time (P < 0.001). There was no significant difference in the proportion of strains containing only pXO1 or strains containing only pXO2 (P = 0.25), but there was a statistical interdependence between the two plasmids (P = 0.004). Loss of viability of B. anthracis cultures stored on agar slants is also discussed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Marston CK,Hoffmaster AR,Wilson KE,Bragg SL,Plikaytis B,Brachman P,Johnson S,Kaufmann AF,Popovic Tdoi
10.1128/AEM.71.12.7778-7780.2005keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
7778-80issue
12eissn
0099-2240issn
1098-5336pii
71/12/7778journal_volume
71pub_type
杂志文章abstract::Various Listeria monocytogenes strains may contaminate a single food product, potentially resulting in simultaneous exposure of consumers to multiple strains. However, due to bias in strain recovery, L. monocytogenes strains isolated from foods by selective enrichment (SE) might not always represent those that can bet...
journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1976-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02549-19
更新日期:2020-04-01 00:00:00
abstract::[This corrects the article on p. 711 in vol. 45.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
abstract::α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...
journal_title:Applied and environmental microbiology
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更新日期:2013-01-01 00:00:00
abstract::Phenanthrene-degrading bacteria were isolated from a 1-m2 intertidal sediment site in Boston Harbor. Samples were taken six times over 2 years. A total of 432 bacteria were isolated and characterized by biochemical testing. When clustered on the basis of phenotypic characteristics, the isolates could be separated into...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.7.2560-2565.1998
更新日期:1998-07-01 00:00:00
abstract::The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2011-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.10.3378-3384.1993
更新日期:1993-10-01 00:00:00
abstract:UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01669-16
更新日期:2016-09-16 00:00:00
abstract::Two different endoxylanases (1,4-beta-D-xylan xylanohydrolases, EC 3.2.1.8), designated 1 and 2, have been purified by column chromatography to apparent homogeneity from the nonsedimentable extracellular culture fluid of the strictly anaerobic, ruminal bacterium Fibrobacter succinogenes S85 grown on crystalline cellul...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.1.157-168.1992
更新日期:1992-01-01 00:00:00