Phylogenetic Relationships and Functional Genes: Distribution of a Gene (mnxG) encoding a putative manganese-oxidizing enzyme in Bacillus species.

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:Bacteria using toxic chemicals, such as detergents, as growth substrates face the challenge of exposing themselves to cell-damaging effects that require protection mechanisms, which demand energy delivered from catabolism of the toxic compound. Thus, adaptations are necessary for ensuring the rapid onset of substrate ...

journal_title：Applied and environmental microbiology

authors： Panasia G,Oetermann S,Steinbüchel A,Philipp B

更新日期：2019-11-14 00:00:00

abstract：:We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activi...

journal_title：Applied and environmental microbiology

authors： Straub PF,Reynolds PH,Althomsons S,Mett V,Zhu Y,Shearer G,Kohl DH

更新日期：1996-01-01 00:00:00

abstract：:To investigate the microbiology and epidemiology of the 1,700 sporadic cases of listeriosis that occur annually in the United States, we developed a multilocus enzyme electrophoresis (MEE) typing system for Listeria monocytogenes. We studied 390 isolates by MEE. Eighty-two electrophoretic types (ETs) were defined. Two...

journal_title：Applied and environmental microbiology

authors： Bibb WF,Gellin BG,Weaver R,Schwartz B,Plikaytis BD,Reeves MW,Pinner RW,Broome CV

更新日期：1990-07-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:Mineral soil-based inoculants of Rhizobium meliloti and Rhizobium phaseoli survived better at 4 degrees C than at higher temperatures, but ca. 15% of the cells were viable at 37 degrees C after 27 days. Soil-based inoculants of R. meliloti, R. phaseoli, Rhizobium japonicum, and a cowpea Rhizobium sp. applied to seeds ...

journal_title：Applied and environmental microbiology

authors： Chao WL,Alexander M

更新日期：1984-01-01 00:00:00

abstract：:Cronobacter species are opportunistic pathogens commonly found in the environment. Among the seven Cronobacter species, Cronobacter sakazakii sequence type 4 (ST-4) is predominantly associated with recorded cases of infantile meningitis. This study reports on a 26-month powdered infant formula (PIF) surveillance progr...

journal_title：Applied and environmental microbiology

authors： Yan Q,Wang J,Gangiredla J,Cao Y,Martins M,Gopinath GR,Stephan R,Lampel K,Tall BD,Fanning S

更新日期：2015-07-01 00:00:00

abstract：:Viral pollution in shellfish has been analyzed simultaneously across a wide range of geographical regions, with emphasis on the concomitant variations in physicochemical characteristics and social features. The methods for sample treatment and for the detection of human enteric viruses were optimized by the participat...

journal_title：Applied and environmental microbiology

authors： Formiga-Cruz M,Tofiño-Quesada G,Bofill-Mas S,Lees DN,Henshilwood K,Allard AK,Conden-Hansson AC,Hernroth BE,Vantarakis A,Tsibouxi A,Papapetropoulou M,Furones MD,Girones R

更新日期：2002-12-01 00:00:00

abstract：:Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic ac...

journal_title：Applied and environmental microbiology

authors： Lee-Wickner LJ,Chassy BM

更新日期：1984-11-01 00:00:00

abstract：:7 alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a Eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-Dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). Chenodeoxych...

journal_title：Applied and environmental microbiology

authors： Masuda N,Oda H,Hirano S,Masuda M,Tanaka H

更新日期：1984-04-01 00:00:00

abstract：:The incorporation of tritiated thymidine by five microbial ecosystems and the distribution of tritium into DNA, RNA, and protein were determined. All microbial assemblages tested exhibited significant labeling of RNA and protein (i.e., nonspecific labeling), as determined by differential acid-base hydrolysis. Nonspeci...

journal_title：Applied and environmental microbiology

authors： Brittain AM,Karl DM

更新日期：1990-05-01 00:00:00

abstract：:With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...

journal_title：Applied and environmental microbiology

authors： Restaino L,Myron JJ,Lenovich LM,Bills S,Tscherneff K

更新日期：1984-04-01 00:00:00

abstract：:Concerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third...

journal_title：Applied and environmental microbiology

authors： Ortiz-Marquez JC,Do Nascimento M,Dublan Mde L,Curatti L

更新日期：2012-04-01 00:00:00

abstract：:Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

journal_title：Applied and environmental microbiology

authors： Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

更新日期：1997-01-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:The quorum sensing (QS) system controls bacterial group behaviors in response to cell density. In vibrios, LuxR and AphA are two master QS regulators (MQSRs) controlling gene expression in response to high or low cell density. Other regulators involved in the regulation of these two MQSRs and QS pathways remain to be ...

journal_title：Applied and environmental microbiology

authors： Gao X,Wang X,Mao Q,Xu R,Zhou X,Ma Y,Liu Q,Zhang Y,Wang Q

更新日期：2018-05-31 00:00:00

abstract：:The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different ...

journal_title：Applied and environmental microbiology

authors： Polansky O,Sekelova Z,Faldynova M,Sebkova A,Sisak F,Rychlik I

更新日期：2015-12-28 00:00:00

abstract：:The influence of physiological and environmental factors on the accumulation of oxindole during anaerobic indole metabolism was investigated by high-performance liquid chromatography. Under methanogenic conditions, indole was temporarily converted to oxindole in stoichiometric amounts in media inoculated with three fr...

journal_title：Applied and environmental microbiology

authors： Madsen EL,Francis AJ,Bollag JM

更新日期：1988-01-01 00:00:00

abstract：:The scarcity of fresh water in the Mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Mediterranean Psammocinia sp. sponges, the grea...

journal_title：Applied and environmental microbiology

authors： Gal-Hemed I,Atanasova L,Komon-Zelazowska M,Druzhinina IS,Viterbo A,Yarden O

更新日期：2011-08-01 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence...

journal_title：Applied and environmental microbiology

authors： Pichard SL,Paul JH

更新日期：1993-02-01 00:00:00

abstract：UNLABELLED:High-throughput sequencing (HTS) is revolutionizing environmental surveys of microbial diversity in the three domains of life by providing detailed information on which taxa are present in microbial assemblages. However, it is still unclear how the relative abundance of specific taxa gathered by HTS correlat...

journal_title：Applied and environmental microbiology

authors： Giner CR,Forn I,Romac S,Logares R,de Vargas C,Massana R

更新日期：2016-07-15 00:00:00

abstract：:Legionella pneumophila suspended in tap water was exposed to biocides recommended for inhibiting biological growth in cooling towers and evaporative condensers of air-conditioning systems. Chlorine, 2,2-dibromo-3-nitrilopropionamide, and a compound containing didecyldimethylammonium chloride and isopropanol were effec...

journal_title：Applied and environmental microbiology

authors： Skaliy P,Thompson TA,Gorman GW,Morris GK,McEachern HV,Mackel DC

更新日期：1980-10-01 00:00:00

abstract：:Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...

journal_title：Applied and environmental microbiology

authors： Simonet P,Bosco M,Chapelon C,Moiroud A,Normand P

更新日期：1994-04-01 00:00:00

abstract：:The expression pattern of manganese peroxidases (MnPs) in nitrogen-limited cultures of the saline-tolerant fungus Phlebia sp. strain MG-60 is differentially regulated under hypersaline conditions at the mRNA level. When MG-60 was cultured in nitrogen-limited medium (LNM) containing 3% (wt/vol) sea salts (LN-SSM), high...

journal_title：Applied and environmental microbiology

authors： Kamei I,Daikoku C,Tsutsumi Y,Kondo R

更新日期：2008-05-01 00:00:00

abstract：:A total of 1,794 migrating birds trapped at a coastal site in southern Sweden were sampled for detection of Campylobacter spp. All isolates phenotypically identified as Campylobacter jejuni and a subset of those identified as non-C. jejuni were identified to the species level by PCR-based techniques. C. jejuni was fou...

journal_title：Applied and environmental microbiology

authors： Waldenström J,Broman T,Carlsson I,Hasselquist D,Achterberg RP,Wagenaar JA,Olsen B

更新日期：2002-12-01 00:00:00

abstract：:A group-specific PCR-based denaturing gradient gel electrophoresis (DGGE) method was developed and combined with group-specific clone library analysis to investigate the diversity of the Clostridium leptum subgroup in human feces. PCR products (length, 239 bp) were amplified using C. leptum cluster-specific primers an...

journal_title：Applied and environmental microbiology

authors： Shen J,Zhang B,Wei G,Pang X,Wei H,Li M,Zhang Y,Jia W,Zhao L

更新日期：2006-08-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:Fecal Candida pintolopesii population levels were found to be significantly affected by laparotomy-inoculation-Bollman apparatus restraint, laparotomy-inoculation, and a milk diet. Gastrectomized rats could not support yeast populations; in intact animals, yeast cells failed to colonize the gastrointestinal tract dist...

journal_title：Applied and environmental microbiology

authors： Artwohl J,McClain A,Cera L

更新日期：1988-10-01 00:00:00

abstract：:Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

journal_title：Applied and environmental microbiology

authors： Thomas PM,Golly KF,Virginia RA,Zyskind JW

更新日期：1995-09-01 00:00:00