Gene expression per gene dose, a specific measure of gene expression in aquatic microorganisms.

Abstract:

:A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence in nontarget organisms. Both target mRNA and DNA are recovered from replicate samples and detected by using antisense and sense single-stranded RNA gene probes. For efficient mRNA recovery, the use of Millipore Durapore filters and multiple extractions was necessary. Quantitation was performed by radiometric detection by using a beta-scanner and comparison of the sample signal against target mRNA and DNA standard curves. This method enabled the measurement of expression of the catechol-2,3-dioxygenase gene (xylE) contained on the thermoregulated plasmid pLV1013 in a marine Vibrio strain in culture and in the environment. In studies of the relationship between mRNA levels and enzyme activities, the appearance of enzyme activity lagged behind xylE mRNA synthesis by an hour after temperature induction. This suggests that mRNA analysis is well suited for determining rapid regulation of microbial gene expression at the transcriptional level in water column microbial populations.

journal_name

Appl Environ Microbiol

authors

Pichard SL,Paul JH

doi

10.1128/AEM.59.2.451-457.1993

keywords:

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

451-7

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Development and implementation of a multiplex single-nucleotide polymorphism genotyping assay for detection of virulence-attenuating mutations in the Listeria monocytogenes virulence-associated gene inlA.

    abstract::The virulence factor internalin A (InlA) facilitates the uptake of Listeria monocytogenes by epithelial cells that express the human isoform of E-cadherin. Previous studies identified naturally occurring premature stop codon (PMSC) mutations in inlA and demonstrated that these mutations are responsible for virulence a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01138-08

    authors: Van Stelten A,Nightingale KK

    更新日期:2008-12-01 00:00:00

  • Characterization of a granulovirus isolated from Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae.

    abstract::A granulovirus (GV) isolated from Epinotia aporema (Lepidoptera: Tortricidae)-a major soybean pest-was studied in terms of its main morphological, biochemical, and biological properties. The ovoidal occlusion bodies were 466 by 296 nm in size, and their most prominent protein had an apparent molecular mass of 29 kDa. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3702-3706.2001

    authors: Sciocco-Cap A,Parola AD,Goldberg AV,Ghiringhelli PD,Romanowski V

    更新日期:2001-08-01 00:00:00

  • Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.

    abstract::In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors. The resul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01249-14

    authors: Han R,Li J,Shin HD,Chen RR,Liu L,Du G,Chen J

    更新日期:2014-08-01 00:00:00

  • Bioassay and dose measurement in UV disinfection.

    abstract::A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.872-877.1983

    authors: Qualls RG,Johnson JD

    更新日期:1983-03-01 00:00:00

  • Pressure stabilization of proteins from extreme thermophiles.

    abstract::We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.3.932-939.1994

    authors: Hei DJ,Clark DS

    更新日期:1994-03-01 00:00:00

  • Rate of Pyrite Bioleaching by Thiobacillus ferrooxidans: Results of an Interlaboratory Comparison.

    abstract::Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.3.642-644.1991

    authors: Olson GJ

    更新日期:1991-03-01 00:00:00

  • Diversity of Rickettsiales in the microbiome of the lone star tick, Amblyomma americanum.

    abstract::Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02987-13

    authors: Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

    更新日期:2014-01-01 00:00:00

  • Chelating Properties of Extracellular Polysaccharides from Chlorella spp.

    abstract::Chlorella stigmatophora LB 993 was grown in artificial seawater under controlled conditions. The production of cell wall polysaccharides attached to the cells and dissolved in the growth medium was monitored during algal growth. Preliminary characterization of the dissolved polysaccharides of C. stigmatophora and othe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2953-2956.1987

    authors: Kaplan D,Christiaen D,Arad SM

    更新日期:1987-12-01 00:00:00

  • Environmental and taxonomic bacterial diversity of anaerobic uranium(IV) bio-oxidation.

    abstract::Microorganisms in diverse terrestrial surface and subsurface environments can anaerobically catalyze the oxidative dissolution of uraninite. While a limited quantity (∼5 to 12 μmol liter(-1)) of uranium is oxidatively dissolved in pure culture studies, the metabolism is coupled to electron transport, providing the pot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02539-10

    authors: Weber KA,Thrash JC,Van Trump JI,Achenbach LA,Coates JD

    更新日期:2011-07-01 00:00:00

  • Temperature-driven adaptation of the bacterial community in peat measured by using thymidine and leucine incorporation.

    abstract::The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1116-1122.2001

    authors: Ranneklev SB,Bååth E

    更新日期:2001-03-01 00:00:00

  • Potential for Development of Tolerance by Penicillium digitatum and Penicillium italicum after Repeated Exposure to Potassium Sorbate.

    abstract::Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.919-923.1985

    authors: Schroeder LL,Bullerman LB

    更新日期:1985-10-01 00:00:00

  • Rapid serological identification of Vibrio vulnificus by anti-H coagglutination.

    abstract::Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.6.1299-1304.1986

    authors: Simonson J,Siebeling RJ

    更新日期:1986-12-01 00:00:00

  • Se(VI) reduction and the precipitation of Se(0) by the facultative bacterium Enterobacter cloacae SLD1a-1 are regulated by FNR.

    abstract::The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02542-06

    authors: Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

    更新日期:2007-03-01 00:00:00

  • Microbial communities and functional genes associated with soil arsenic contamination and the rhizosphere of the arsenic-hyperaccumulating plant Pteris vittata L.

    abstract::To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00500-10

    authors: Xiong J,Wu L,Tu S,Van Nostrand JD,He Z,Zhou J,Wang G

    更新日期:2010-11-01 00:00:00

  • Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on vSaβ typing.

    abstract::Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02662-20

    authors: Schwendimann L,Merda D,Berger T,Denayer S,Feraudet Tarisse C,Kläui AJ,Messio S,Mistou MY,Nia Y,Hennekinne JA,Graber HU

    更新日期:2020-12-18 00:00:00

  • Culturing enterohemorrhagic Escherichia coli in the presence and absence of glucose as a simple means of evaluating the acid tolerance of stationary-phase cells.

    abstract::Prior growth of seven enterohemorrhagic and one nonenterohemorrhagic strains of Escherichia coli in tryptic soy broth with (TSB+G) and without (TSB-G) 1% glucose was evaluated for its effect on acid tolerance. The final pHs of 18-h TSB+G and TSB-G cultures were 4.6 to 5.2 and 6.9 to 7.0, respectively. Cells were then ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.11.4009-4013.1996

    authors: Buchanan RL,Edelson SG

    更新日期:1996-11-01 00:00:00

  • Molecular monitoring of the fecal microbiota of healthy human subjects during administration of lactulose and Saccharomyces boulardii.

    abstract::Diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. In this study, a long-term placebo-controlled cro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00233-06

    authors: Vanhoutte T,De Preter V,De Brandt E,Verbeke K,Swings J,Huys G

    更新日期:2006-09-01 00:00:00

  • Production of medium-chain-length poly(3-hydroxyalkanoates) from gluconate by recombinant Escherichia coli.

    abstract::It was shown recently that recombinant Escherichia coli, defective in the beta-oxidation cycle and harboring a medium-chain-length (MCL) poly(3-hydroxyalkanoate) (PHA) polymerase-encoding gene of Pseudomonas, is able to produce MCL PHA from fatty acids but not from sugars or gluconate (S. Langenbach, B. H. A. Rehm, an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.540-548.1999

    authors: Klinke S,Ren Q,Witholt B,Kessler B

    更新日期:1999-02-01 00:00:00

  • A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

    abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4571-4574.2000

    authors: Bernhard AE,Field KG

    更新日期:2000-10-01 00:00:00

  • Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability.

    abstract::The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity to several well-chara...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.2764-2770.2004

    authors: Cai G,Zhu S,Yang S,Zhao G,Jiang W

    更新日期:2004-05-01 00:00:00

  • Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing.

    abstract:UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00411-16

    authors: Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

    更新日期:2016-05-16 00:00:00

  • Unprotonated Short-Chain Alkylamines Inhibit Staphylolytic Activity of Lysostaphin in a Wall Teichoic Acid-Dependent Manner.

    abstract::Lysostaphin (Lst) is a potent bacteriolytic enzyme that kills Staphylococcus aureus, a common bacterial pathogen of humans and animals. With high activity against both planktonic cells and biofilms, Lst has the potential to be used in industrial products, such as commercial cleansers, for decontamination. However, Lst...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00693-18

    authors: Wu X,Kwon SJ,Kim D,Zha J,Mora-Pale M,Dordick JS

    更新日期:2018-07-02 00:00:00

  • Respiratory Physiology of Lactococcus lactis in Chemostat Cultures and Its Effect on Cellular Robustness in Frozen and Freeze-Dried Starter Cultures.

    abstract::In this study, we used chemostat cultures to analyze the quantitative effects of the specific growth rate and respiration on the metabolism in Lactococcus lactis CHCC2862 and on the downstream robustness of cells after freezing or freeze-drying. Under anaerobic conditions, metabolism remained homofermentative, althoug...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02785-19

    authors: Johanson A,Goel A,Olsson L,Franzén CJ

    更新日期:2020-03-02 00:00:00

  • Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.

    abstract::DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.4.1378-1382.1996

    authors: Coleman SS,Melanson DM,Biosca EG,Oliver JD

    更新日期:1996-04-01 00:00:00

  • Temperature affects species distribution in symbiotic populations of Vibrio spp.

    abstract::The genus Sepiola (Cephalopoda: Sepiolidae) contains 10 known species that occur in the Mediterranean Sea today. All Sepiola species have a light organ that contains at least one of two species of luminous bacteria, Vibrio fischeri and Vibrio logei. The two Vibrio species coexist in at least four Sepiola species (S. a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3550-3555.2000

    authors: Nishiguchi MK

    更新日期:2000-08-01 00:00:00

  • Enhancing the production of hydroxyl radicals by Pleurotus eryngii via quinone redox cycling for pollutant removal.

    abstract::The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02138-08

    authors: Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

    更新日期:2009-06-01 00:00:00

  • Acetogenic and sulfate-reducing bacteria inhabiting the rhizoplane and deep cortex cells of the sea grass Halodule wrightii.

    abstract::Recent declines in sea grass distribution underscore the importance of understanding microbial community structure-function relationships in sea grass rhizospheres that might affect the viability of these plants. Phospholipid fatty acid analyses showed that sulfate-reducing bacteria and clostridia were enriched in sed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.11.5117-5123.1999

    authors: Küsel K,Pinkart HC,Drake HL,Devereux R

    更新日期:1999-11-01 00:00:00

  • Community analysis of a mercury hot spring supports occurrence of domain-specific forms of mercuric reductase.

    abstract::Mercury is a redox-active heavy metal that reacts with active thiols and depletes cellular antioxidants. Active resistance to the mercuric ion is a widely distributed trait among bacteria and results from the action of mercuric reductase (MerA). Protein phylogenetic analysis of MerA in bacteria indicated the occurrenc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8836-8845.2005

    authors: Simbahan J,Kurth E,Schelert J,Dillman A,Moriyama E,Jovanovich S,Blum P

    更新日期:2005-12-01 00:00:00

  • Public health assessment of Salmonella enterica serovar enteritidis inactivated-vaccine treatment in layer flocks.

    abstract::Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01689-08

    authors: Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

    更新日期:2009-02-01 00:00:00

  • Molecular and physiological responses of two classes of marine chromophytic phytoplankton (Diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms.

    abstract::Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2349-2357.2000

    authors: Wyman M,Davies JT,Crawford DW,Purdie DA

    更新日期:2000-06-01 00:00:00