Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations.

abstract：:The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

journal_title：Applied and environmental microbiology

authors： Abe J,Ushijima C,Hizukuri S

更新日期：1999-09-01 00:00:00

abstract：:Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M Na...

journal_title：Applied and environmental microbiology

authors： Korber DR,Choi A,Wolfaardt GM,Caldwell DE

更新日期：1996-11-01 00:00:00

abstract：:To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

journal_title：Applied and environmental microbiology

authors： Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

更新日期：2014-12-01 00:00:00

abstract：:A xanthanase complex secreted by a consortium of heat-stable, salt-tolerant bacteria includes a lyase that specifically removes terminal pyruvated beta-d-mannose residues from the side chains of xanthan gum. The enzyme was purified to homogeneity from the culture broth following ion-exchange chromatography and gel per...

journal_title：Applied and environmental microbiology

authors： Ahlgren JA

更新日期：1991-09-01 00:00:00

abstract：:Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found within the Fe(III) reduction zone of subsurface environments. Culture filtrates of G. fermentans stimulated the reduction of poorly crystalline Fe(III) oxide by washed cell suspensions,...

journal_title：Applied and environmental microbiology

authors： Nevin KP,Lovley DR

更新日期：2002-05-01 00:00:00

abstract：:The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...

journal_title：Applied and environmental microbiology

authors： Phelps PA,Agarwal SK,Speitel GE,Georgiou G

更新日期：1992-11-01 00:00:00

abstract：:The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect f...

journal_title：Applied and environmental microbiology

authors： Busta FF,Baillie E,Murrell WG

更新日期：1976-08-01 00:00:00

abstract：:The aim of this study was to investigate the phenotypic and genotypic antibiotic resistance profiles of pseudomonads isolated from surfaces of a goat and lamb slaughterhouse, which were representative of areas that are possible sources of meat contamination. Mesophilic (85 isolates) and psychrotrophic (37 isolates) ps...

journal_title：Applied and environmental microbiology

authors： Lavilla Lerma L,Benomar N,Casado Muñoz Mdel C,Gálvez A,Abriouel H

更新日期：2014-11-01 00:00:00

abstract：:Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

journal_title：Applied and environmental microbiology

authors： Elhanafi D,Dutta V,Kathariou S

更新日期：2010-12-01 00:00:00

abstract：:The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest ...

journal_title：Applied and environmental microbiology

authors： Frostegård A,Tunlid A,Bååth E

更新日期：1993-11-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

journal_title：Applied and environmental microbiology

authors： Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

更新日期：1993-06-01 00:00:00

abstract：:A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...

journal_title：Applied and environmental microbiology

authors： Dreyfus BL,Elmerich C,Dommergues YR

更新日期：1983-02-01 00:00:00

abstract：:The relationship between the production of reduced oxygen species, hydrogen peroxide (H(2)O(2)), superoxide (O(2)), and hydroxyl radical (.OH), and the oxidation of synthetic lignin to CO(2) was studied in whole cultures of the white-rot fungus Phanerochaete chrysosporium Burds. The kinetics of the synthesis of H(2)O(...

journal_title：Applied and environmental microbiology

authors： Faison BD,Kirk TK

更新日期：1983-11-01 00:00:00

abstract：:Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pP(x)GM(iii)) and a decrease in that of pP(x)GM(ii). The magnitude of (31)P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1-O-[N...

journal_title：Applied and environmental microbiology

authors： Park YI,Bonetti SJ,Gander JE

更新日期：2000-02-01 00:00:00

abstract：:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose cont...

journal_title：Applied and environmental microbiology

authors： Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

更新日期：2011-08-01 00:00:00

abstract：:Four sourdoughs (A to D) were produced under practical conditions by using a starter mixture of three commercially available sourdough starters and a baker's yeast constitutively containing various species of lactic acid bacteria (LAB). The sourdoughs were continuously propagated until the composition of the LAB flora...

journal_title：Applied and environmental microbiology

authors： Meroth CB,Walter J,Hertel C,Brandt MJ,Hammes WP

更新日期：2003-01-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitr...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Anda M,Inaba S,Eda S,Sato S,Sasaki K,Tabata S,Mitsui H,Sato T,Shinano T,Minamisawa K

更新日期：2011-03-01 00:00:00

abstract：:Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

journal_title：Applied and environmental microbiology

authors： Ng TK,Zeikus JG

更新日期：1986-10-01 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopep...

journal_title：Applied and environmental microbiology

authors： Flambard B,Juillard V

更新日期：2000-12-01 00:00:00

abstract：:An obligately anaerobic bacterium which transforms several chlorinated phenols was isolated. Dechlorination of the substituents ortho to the phenolic OH group was preferred, while removal of a meta-substituted chlorine was observed only with 3,5-dichlorophenol. The bacterium was a gram-positive, endospore-forming, mot...

journal_title：Applied and environmental microbiology

authors： Madsen T,Licht D

更新日期：1992-09-01 00:00:00

abstract：:Most strains of Butyrivibrio fibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields range...

journal_title：Applied and environmental microbiology

authors： Ha YW,Stack RJ,Hespell RB,Gordon SH,Bothast RJ

更新日期：1991-07-01 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00

abstract：:The expression of xenobiotic-degradative genes in indigenous bacteria or in bacteria introduced into an ecosystem is essential for the successful bioremediation of contaminated environments. The maintenance of naphthalene utilization activity is studied in Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. (strain...

journal_title：Applied and environmental microbiology

authors： Guerin WF,Boyd SA

更新日期：1995-11-01 00:00:00

abstract：:The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Oberleiter A,Psenner R

更新日期：1996-12-01 00:00:00

abstract：UNLABELLED:In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mut...

journal_title：Applied and environmental microbiology

authors： Huertas-Rosales Ó,Ramos-González MI,Espinosa-Urgel M

更新日期：2016-08-30 00:00:00

abstract：:The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sou...

journal_title：Applied and environmental microbiology

authors： Bannerman E,Yersin MN,Bille J

更新日期：1992-06-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are widely produced by Gram-positive bacteria, including many species of the Bacillus group. In the present study, one novel gene cluster coding lantibiotic cerecidins was unveiled in Bacillus cereus strain As 1.1846 ...

journal_title：Applied and environmental microbiology

authors： Wang J,Zhang L,Teng K,Sun S,Sun Z,Zhong J

更新日期：2014-04-01 00:00:00