Cerecidins, novel lantibiotics from Bacillus cereus with potent antimicrobial activity.

abstract：:The solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. In common with other anaerobes, the clostridia show a marked dependence on the phosphoeno...

journal_title：Applied and environmental microbiology

authors： Al Makishah NH,Mitchell WJ

更新日期：2013-11-01 00:00:00

abstract：:An extracellular serine proteinase from Brevibacterium linens ATCC 9174 was purified to homogeneity. pH and temperature optima were 8.5 and 50(deg)C, respectively. The results for the molecular mass of the proteinase were 56 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 126 kDa by gel filtration...

journal_title：Applied and environmental microbiology

authors： Rattray FP,Bockelmann W,Fox PF

更新日期：1995-09-01 00:00:00

abstract：:Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...

journal_title：Applied and environmental microbiology

authors： Schwendener S,Perreten V

更新日期：2015-05-01 00:00:00

abstract：:The activity of glutaraldehyde (GTA) at low concentrations (less than 2%) against poliovirus was assessed by a suspension procedure. The inactivation kinetics showed that concentrations of less than or equal to 0.10% were effective against purified poliovirus at pH 7.2; a 1 log10 reduction was obtained in 70 min with ...

journal_title：Applied and environmental microbiology

authors： Bailly JL,Chambon M,Peigue-Lafeuille H,Laveran H,De Champs C,Beytout D

更新日期：1991-04-01 00:00:00

abstract：:Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis s...

journal_title：Applied and environmental microbiology

authors： Aronson JN,Arvidson HC

更新日期：1987-02-01 00:00:00

abstract：:Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. S...

journal_title：Applied and environmental microbiology

authors： Lin JL,Joye SB,Scholten JC,Schäfer H,McDonald IR,Murrell JC

更新日期：2005-10-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:The potential of Pseudomonas pseudoflava to produce poly-beta-hydroxyalkanoates (PHAs) from pentoses was studied. This organism was able to use a hydrolysate from the hemicellulosic fraction of poplar wood as a carbon and energy source for its growth. However, in batch cultures, growth was inhibited completely at hydr...

journal_title：Applied and environmental microbiology

authors： Bertrand JL,Ramsay BA,Ramsay JA,Chavarie C

更新日期：1990-10-01 00:00:00

abstract：:Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experime...

journal_title：Applied and environmental microbiology

authors： Dwulit-Smith JR,Hamilton JJ,Stevenson DM,He S,Oyserman BO,Moya-Flores F,Garcia SL,Amador-Noguez D,McMahon KD,Forest KT

更新日期：2018-11-30 00:00:00

abstract：:The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...

journal_title：Applied and environmental microbiology

authors： Domenech M,García E

更新日期：2020-05-05 00:00:00

abstract：:We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Apajalahti JH,Salkinoja-Salonen MS

更新日期：1988-03-01 00:00:00

abstract：:Cyanobacterial blooms are potential health hazards in water supply reservoirs. This paper reports analyses of a cyanobacterial bloom by use of PCR-based methods for direct detection and identification of strains present and determination of their toxigenicity. Serial samples from Malpas Dam, in the New England region ...

journal_title：Applied and environmental microbiology

authors： Baker JA,Entsch B,Neilan BA,McKay DB

更新日期：2002-12-01 00:00:00

abstract：:Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to un...

journal_title：Applied and environmental microbiology

authors： Fischer M,Sawers RG

更新日期：2013-07-01 00:00:00

abstract：:Methanogenic isolates did not consume hydrogen below partial pressures of 6.5 Pa. Thus, in contrast to a previous report, results from pure-culture studies do not invalidate the threshold model for methane production from hydrogen in sediments. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR

更新日期：1985-06-01 00:00:00

abstract：:Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

journal_title：Applied and environmental microbiology

authors： Huang S,Zhang S,Jiao N,Chen F

更新日期：2015-01-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00

abstract：:Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

journal_title：Applied and environmental microbiology

authors： Cole DF,Bugbee WM

更新日期：1976-05-01 00:00:00

abstract：:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number o...

journal_title：Applied and environmental microbiology

authors： Sanders SW,Maxcy RB

更新日期：1979-09-01 00:00:00

abstract：:The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertili...

journal_title：Applied and environmental microbiology

authors： Chu H,Fujii T,Morimoto S,Lin X,Yagi K,Hu J,Zhang J

更新日期：2007-01-01 00:00:00

abstract：:Fenton reactions are believed to play important roles in wood degradation by brown rot fungi. In this context, the effect of tropolone (2-hydroxycyclohepta-2,4,6-trienone), a metal chelator, on wood degradation by Poria placenta was investigated. Tropolone (50 micro M) strongly inhibits fungal growth on malt agar, but...

journal_title：Applied and environmental microbiology

authors： Diouf PN,Delbarre N,Perrin D,Gérardin P,Rapin C,Jacquot JP,Gelhaye E

更新日期：2002-09-01 00:00:00

abstract：:Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

journal_title：Applied and environmental microbiology

authors： He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

更新日期：2012-07-01 00:00:00

abstract：UNLABELLED:Neorickettsia (formerly Ehrlichia) risticii is an obligatory intracellular bacterium of digenetic trematodes. When a horse accidentally ingests aquatic insects containing encysted trematodes infected with N. risticii, the bacterium is transmitted from trematodes to horse cells and causes an acute and often f...

journal_title：Applied and environmental microbiology

authors： Xiong Q,Bekebrede H,Sharma P,Arroyo LG,Baird JD,Rikihisa Y

更新日期：2016-09-16 00:00:00

abstract：:Seven organotin compounds and tin chloride were tested for their effects on the methanogenic bacteria Methanococcus thermolithotrophicus, Methanococcus deltae delta LH, and Methanosarcina barkeri 227. The methanogens were strongly inhibited by triethyltin, tripropyltin, and monophenyltin compounds, generally at concen...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-04-01 00:00:00

abstract：:Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

journal_title：Applied and environmental microbiology

authors： Halpern M,Gancz H,Broza M,Kashi Y

更新日期：2003-07-01 00:00:00

abstract：:We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...

journal_title：Applied and environmental microbiology

authors： Pankowski JA,Puckett SM,Nano FE

更新日期：2016-01-15 00:00:00

abstract：:The present investigation showed by means of autoradiography that the cyanobacterium Microcystis wesenbergii did not incorporate [H]thymidine at nanomolar concentrations, whereas its associated heterotrophic bacteria appearing in the gelatinous cover of the cyanobacterium became labeled. Several other tested cyaobacte...

journal_title：Applied and environmental microbiology

authors： Bern L

更新日期：1985-01-01 00:00:00

abstract：:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detect...

journal_title：Applied and environmental microbiology

authors： Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg H

更新日期：2015-12-11 00:00:00

abstract：:Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to ...

journal_title：Applied and environmental microbiology

authors： Newell SY,Arsuffi TL,Fallon RD

更新日期：1988-07-01 00:00:00

abstract：:Inorganic phosphorus (Pi) is one of the main growth-limiting factors of diazotrophic cyanobacteria. Due to human activity, the availability of Pi has increased in water bodies, resulting in eutrophication and the formation of massive cyanobacterial blooms. In this study, we examined the molecular responses of the cyan...

journal_title：Applied and environmental microbiology

authors： Teikari J,Österholm J,Kopf M,Battchikova N,Wahlsten M,Aro EM,Hess WR,Sivonen K

更新日期：2015-08-01 00:00:00

abstract：:Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

journal_title：Applied and environmental microbiology

authors： Muñoz G,Sotomayor P

更新日期：1990-07-01 00:00:00