Conditions for growing Mycoplasma canadense and Mycoplasma verecundum in a serum-free medium.

abstract：:An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic aci...

journal_title：Applied and environmental microbiology

authors： Nelson KE,Pell AN,Schofield P,Zinder S

更新日期：1995-09-01 00:00:00

abstract：:Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that alpha/beta-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist ...

journal_title：Applied and environmental microbiology

authors： Paredes-Sabja D,Sarker N,Setlow B,Setlow P,Sarker MR

更新日期：2008-06-01 00:00:00

abstract：:Several cell surface characteristics of bacteriophage-resistant Lactococcus lactis subsp. cremoris SK110 were compared with those of its phage-sensitive derivative SK112. After centrifugation, SK110 cells resisted suspension more strongly than SK112 cells. SK112 was more negatively charged and had a more hydrophobic c...

journal_title：Applied and environmental microbiology

authors： Sijtsma L,Jansen N,Hazeleger WC,Wouters JT,Hellingwerf KJ

更新日期：1990-10-01 00:00:00

abstract：:Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted ...

journal_title：Applied and environmental microbiology

authors： Campa MF,Techtmann SM,Ladd MP,Yan J,Patterson M,Garcia de Matos Amaral A,Carter KE,Ulrich N,Grant CJ,Hettich RL,Lamendella R,Hazen TC

更新日期：2019-10-16 00:00:00

abstract：:The growth response of Acacia mangium Willd. to inoculation with selected Bradyrhizobium strains was investigated in two field trials in the Ivory Coast (West Africa). In the first trial (Anguededou), four provenances (i.e., trees originating from seeds harvested in different geographical areas) of A. mangium were ino...

journal_title：Applied and environmental microbiology

authors： Galiana A,Prin Y,Mallet B,Gnahoua GM,Poitel M,Diem HG

更新日期：1994-11-01 00:00:00

abstract：:2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4...

journal_title：Applied and environmental microbiology

authors： Seffernick JL,Shapir N,Schoeb M,Johnson G,Sadowsky MJ,Wackett LP

更新日期：2002-09-01 00:00:00

abstract：:Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

journal_title：Applied and environmental microbiology

authors： Fallon RD

更新日期：1992-09-01 00:00:00

abstract：:"Candidatus Accumulibacter" is the dominant polyphosphate-accumulating organism (PAO) in denitrifying phosphorus removal (DPR) systems. In order to investigate the community structure and clade morphotypes of "Candidatus Accumulibacter" in DPR systems through flow cytometry (FCM), denitrifying phosphorus removal of al...

journal_title：Applied and environmental microbiology

authors： Li C,Zeng W,Li N,Guo Y,Peng Y

更新日期：2019-04-18 00:00:00

abstract：:Aerobiological monitoring was conducted in an experimental room to aid in the development of standardized sampling protocols for airborne microorganisms in the indoor environment. The objectives of this research were to evaluate the relative efficiencies of selected sampling methods for the retrieval of airborne funga...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Stetzenbach LD

更新日期：1993-01-01 00:00:00

abstract：:Most strains of Butyrivibrio fibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields range...

journal_title：Applied and environmental microbiology

authors： Ha YW,Stack RJ,Hespell RB,Gordon SH,Bothast RJ

更新日期：1991-07-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown ...

journal_title：Applied and environmental microbiology

authors： Suar M,van der Meer JR,Lawlor K,Holliger C,Lal R

更新日期：2004-11-01 00:00:00

abstract：:In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50...

journal_title：Applied and environmental microbiology

authors： Tan Y,Miller KJ

更新日期：1992-04-01 00:00:00

abstract：:A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for me...

journal_title：Applied and environmental microbiology

authors： Gallardo O,Fernández-Fernández M,Valls C,Valenzuela SV,Roncero MB,Vidal T,Díaz P,Pastor FI

更新日期：2010-09-01 00:00:00

abstract：:Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

journal_title：Applied and environmental microbiology

authors： Gilliland SE,Speck ML

更新日期：1977-01-01 00:00:00

abstract：:Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

journal_title：Applied and environmental microbiology

authors： Cao Y,Green PG,Holden PA

更新日期：2008-12-01 00:00:00

abstract：:3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as its sole sources of carbon, nitrogen, and energy to grow, but the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, might be respons...

journal_title：Applied and environmental microbiology

authors： Wang H,Wang X,Ren H,Wang X,Lu Z

更新日期：2020-09-17 00:00:00

abstract：:Phages play a key role in the marine environment by regulating the transfer of energy between trophic levels and influencing global carbon and nutrient cycles. The diversity of marine phage communities remains difficult to characterize because of the lack of a signature gene common to all phages. Recent studies have d...

journal_title：Applied and environmental microbiology

authors： Goldsmith DB,Crosti G,Dwivedi B,McDaniel LD,Varsani A,Suttle CA,Weinbauer MG,Sandaa RA,Breitbart M

更新日期：2011-11-01 00:00:00

abstract：:The proportion of oocysts of Cryptosporidium parvum showing a fold on oocyst walls when incubated with either fluorescent monoclonal antibody or a surface-reactive fluorescent dye was increased by incubating suspensions of oocysts with dimethyl sulfoxide, sucrose, or Hanks' balanced salt solution. Further incubation o...

journal_title：Applied and environmental microbiology

authors： Robertson LJ,Campbell AT,Smith HV

更新日期：1993-08-01 00:00:00

abstract：:In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

journal_title：Applied and environmental microbiology

authors： Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

更新日期：2008-04-01 00:00:00

abstract：:The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...

journal_title：Applied and environmental microbiology

authors： Lillehaug D,Lindqvist B,Birkeland NK

更新日期：1991-11-01 00:00:00

abstract：:Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover a...

journal_title：Applied and environmental microbiology

authors： Wang Y,Wang Z,Cao J,Guan Z,Lu X

更新日期：2014-10-01 00:00:00

abstract：:The phylogenetic diversity of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, E.C. 4.1.1.39) large-subunit genes of deep-sea microorganisms was analyzed. Bulk genomic DNA was isolated from seven samples, including samples from the Mid-Atlantic Ridge and various deep-sea habitats around Japan. The kinds o...

journal_title：Applied and environmental microbiology

authors： Elsaied H,Naganuma T

更新日期：2001-04-01 00:00:00

abstract：:Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic ac...

journal_title：Applied and environmental microbiology

authors： Lee-Wickner LJ,Chassy BM

更新日期：1984-11-01 00:00:00

abstract：:To longitudinally assess fecal shedding and animal-to-animal transmission of Clostridium difficile among finishing feedlot cattle as a risk for beef carcass contamination, we tested 186 ± 12 steers (mean ± standard deviation; 1,369 samples) in an experimental feedlot facility during the finishing period and at harvest...

journal_title：Applied and environmental microbiology

authors： Rodriguez-Palacios A,Pickworth C,Loerch S,LeJeune JT

更新日期：2011-05-01 00:00:00

abstract：:Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

journal_title：Applied and environmental microbiology

authors： Bardell D

更新日期：1978-11-01 00:00:00

abstract：:We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coa...

journal_title：Applied and environmental microbiology

authors： Kitajima M,Oka T,Haramoto E,Katayama H,Takeda N,Katayama K,Ohgaki S

更新日期：2010-04-01 00:00:00

abstract：:Thirty-three standard mycotoxins were assayed by thin-layer chromatography and by cytotoxicity in HEp-2 and Chang cells. Various levels of detection were found. The cytotoxicity test was significantly more sensitive than thin-layer chromatography for the trichothecenes and should be useful for screening extracts from ...

journal_title：Applied and environmental microbiology

authors： Robb J,Norval M

更新日期：1983-10-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00

abstract：:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

更新日期：2005-12-01 00:00:00