FLP-FRT-based method to obtain unmarked deletions of CHU_3237 (porU) and large genomic fragments of Cytophaga hutchinsonii.

Abstract:

:Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover and FLP-FRT recombination system to obtain unmarked deletions of both single genes and large genomic fragments in C. hutchinsonii. Unmarked deletion of CHU_3237 (porU), an ortholog of the C-terminal signal peptidase of a type IX secretion system (T9SS), resulted in defects in colony spreading, cellulose degradation, and protein secretion, indicating that it is a component of the T9SS and that T9SS plays an important role in cellulose degradation by C. hutchinsonii. Furthermore, deletions of four large genomic fragments were obtained using our method, and the sizes of the excised fragments varied from 9 to 19 kb, spanning from 6 to 22 genes. The customized FLP-FRT method provides an efficient tool for more rapid progress in the cellulose degradation mechanism and other physiological aspects of C. hutchinsonii.

journal_name

Appl Environ Microbiol

authors

Wang Y,Wang Z,Cao J,Guan Z,Lu X

doi

10.1128/AEM.01785-14

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

6037-45

issue

19

eissn

0099-2240

issn

1098-5336

pii

AEM.01785-14

journal_volume

80

pub_type

杂志文章
  • Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective

    abstract::The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.5020-5022.1998

    authors: Robleto EA,Borneman J,Triplett EW

    更新日期:1998-12-01 00:00:00

  • A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

    abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4571-4574.2000

    authors: Bernhard AE,Field KG

    更新日期:2000-10-01 00:00:00

  • D-2,3-butanediol production due to heterologous expression of an acetoin reductase in Clostridium acetobutylicum.

    abstract::Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding A...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01616-10

    authors: Siemerink MA,Kuit W,López Contreras AM,Eggink G,van der Oost J,Kengen SW

    更新日期:2011-04-01 00:00:00

  • The secreted esterase of Propionibacterium freudenreichii has a major role in cheese lipolysis.

    abstract::Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03640-13

    authors: Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

    更新日期:2014-01-01 00:00:00

  • Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals.

    abstract::Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a repo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.5.1858-1863.1999

    authors: Mach RL,Peterbauer CK,Payer K,Jaksits S,Woo SL,Zeilinger S,Kullnig CM,Lorito M,Kubicek CP

    更新日期:1999-05-01 00:00:00

  • N-demethylation of nicotine and reduction of nicotine-1'-N-oxide by Microsporum gypseum.

    abstract::Several microorganisms were examined for their abilities to convert S-nicotine into nornicotine. Five microorganisms including Microsporum gypseum (ATCC 11395) produced nornicotine and three unknown metabolites. M. gypseum efficiently reduced nicotine-1'-N-oxide to nicotine, but no nornicotine was obtained when the N-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.5.836-839.1979

    authors: Sindelar RD,Rosazza JP,Barfknecht CF

    更新日期:1979-11-01 00:00:00

  • Competing isogenic Campylobacter strains exhibit variable population structures in vivo.

    abstract::Consumption of poultry contaminated with Campylobacter jejuni is a risk factor for human gastrointestinal disease. The rational development of control strategies for Campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02835-07

    authors: Coward C,van Diemen PM,Conlan AJ,Gog JR,Stevens MP,Jones MA,Maskell DJ

    更新日期:2008-06-01 00:00:00

  • Products from the incomplete metabolism of pyrene by polycyclic aromatic hydrocarbon-degrading bacteria.

    abstract::Pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (PAH). It is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other PAH-degrading bacteria. We examined the formation of such products by four bacterial strains and identified and furt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.1917-1922.2000

    authors: Kazunga C,Aitken MD

    更新日期:2000-05-01 00:00:00

  • Genomic island TnSmu2 of Streptococcus mutans harbors a nonribosomal peptide synthetase-polyketide synthase gene cluster responsible for the biosynthesis of pigments involved in oxygen and H2O2 tolerance.

    abstract::The oral biofilm community consists of >800 microbial species, among which Streptococcus mutans is considered a primary pathogen for dental caries. The genomic island TnSmu2 of S. mutans comprises >2% of the genome. In this study, we demonstrate that TnSmu2 harbors a gene cluster encoding nonribosomal peptide syntheta...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03079-09

    authors: Wu C,Cichewicz R,Li Y,Liu J,Roe B,Ferretti J,Merritt J,Qi F

    更新日期:2010-09-01 00:00:00

  • Identification and characterization of a novel flagellum-dependent Salmonella-infecting bacteriophage, iEPS5.

    abstract::A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00706-13

    authors: Choi Y,Shin H,Lee JH,Ryu S

    更新日期:2013-08-01 00:00:00

  • Pyranose Oxidase, a Major Source of H(2)O(2) during Wood Degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida.

    abstract::The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.7.2524-2532.1994

    authors: Daniel G,Volc J,Kubatova E

    更新日期:1994-07-01 00:00:00

  • Characterization of a granulovirus isolated from Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae.

    abstract::A granulovirus (GV) isolated from Epinotia aporema (Lepidoptera: Tortricidae)-a major soybean pest-was studied in terms of its main morphological, biochemical, and biological properties. The ovoidal occlusion bodies were 466 by 296 nm in size, and their most prominent protein had an apparent molecular mass of 29 kDa. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3702-3706.2001

    authors: Sciocco-Cap A,Parola AD,Goldberg AV,Ghiringhelli PD,Romanowski V

    更新日期:2001-08-01 00:00:00

  • Neutral sugar composition and gravimetric yield of plant and bacterial fractions of feces.

    abstract::Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.6.1786-1792.1990

    authors: Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

    更新日期:1990-06-01 00:00:00

  • Novel polyketide synthase from Nectria haematococca.

    abstract::We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.2984-2988.2004

    authors: Graziani S,Vasnier C,Daboussi MJ

    更新日期:2004-05-01 00:00:00

  • Cooccurrence of ScDSP gene expression, cell death, and DNA fragmentation in a marine diatom, Skeletonema costatum.

    abstract::A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8744-8751.2005

    authors: Chung CC,Hwang SP,Chang J

    更新日期:2005-12-01 00:00:00

  • The role of host phylogeny varies in shaping microbial diversity in the hindguts of lower termites.

    abstract::The hindguts of lower termites and Cryptocercus cockroaches are home to a distinct community of archaea, bacteria, and protists (primarily parabasalids and some oxymonads). Within a host species, the composition of these hindgut communities appears relatively stable, but the evolutionary and ecological factors structu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02945-14

    authors: Tai V,James ER,Nalepa CA,Scheffrahn RH,Perlman SJ,Keeling PJ

    更新日期:2015-02-01 00:00:00

  • Elution of viruses by ionic and nonionic surfactants.

    abstract::The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3470-3473.1996

    authors: Fujito BT,Lytle CD

    更新日期:1996-09-01 00:00:00

  • Microbiological aspects of aerobic thermophilic treatment of swine waste.

    abstract::A thermophilic strain (D2) identified as a Bacillus sp. was isolated from an aerobic digestor of swine waste after several months of operation at 55 degrees C. Aerobic thermophilic batch treatment of swine waste inoculated with strain D2 was studied in a 4-liter fixed-bed reactor. Stabilization of the waste was achiev...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.971-976.1990

    authors: Beaudet R,Gagnon C,Bisaillon JG,Ishaque M

    更新日期:1990-04-01 00:00:00

  • Fermentative Degradation of Polyethylene Glycol by a Strictly Anaerobic, Gram-Negative, Nonsporeforming Bacterium, Pelobacter venetianus sp. nov.

    abstract::[This corrects the article on p. 1913 in vol. 45.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.2.532-532.1983

    authors:

    更新日期:1983-08-01 00:00:00

  • Rectal administration of Escherichia coli O157:H7: novel model for colonization of ruminants.

    abstract::Escherichia coli O157:H7 causes hemorrhagic colitis and life-threatening complications. Because healthy cattle are reservoirs for the bacterium, ruminant infection models have applications in analyzing the relationship between cattle and this human pathogen and in testing interventions to reduce or prevent bovine colo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4588-4595.2004

    authors: Sheng H,Davis MA,Knecht HJ,Hovde CJ

    更新日期:2004-08-01 00:00:00

  • Effects of Agricultural Management on Rhizosphere Microbial Structure and Function in Processing Tomato Plants.

    abstract::Agricultural management practices affect bulk soil microbial communities and the functions they carry out, but it remains unclear how these effects extend to the rhizosphere in different agroecosystem contexts. Given close linkages between rhizosphere processes and plant nutrition and productivity, understanding how m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01064-19

    authors: Schmidt JE,Vannette RL,Igwe A,Blundell R,Casteel CL,Gaudin ACM

    更新日期:2019-08-01 00:00:00

  • Common occurrence of plasmid DNA and vancomycin resistance in Leuconostoc spp.

    abstract::Resistance to vancomycin permitted detection, in a culture of Streptococcus cremoris 290PC, of a contaminant gram-positive coccus. Morphological and physiological characteristics indicated that this bacterium was a strain of Leuconostoc sp., designated PO184. This strain contained four plasmid species, which were dist...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.6.1129-1133.1984

    authors: Orberg PK,Sandine WE

    更新日期:1984-12-01 00:00:00

  • Fecal Microbiotas of Indonesian and New Zealand Children Differ in Complexity and Bifidobacterial Taxa during the First Year of Life.

    abstract::The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01105-19

    authors: Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

    更新日期:2019-09-17 00:00:00

  • Binding rather than metabolism may explain the interaction of two food-Grade Lactobacillus strains with zearalenone and its derivative (')alpha-earalenol.

    abstract::The interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative (')alpha-zearalenol ((')alpha-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 microg ml(-1)) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A consider...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.7.3545-3549.2002

    authors: El-Nezami H,Polychronaki N,Salminen S,Mykkänen H

    更新日期:2002-07-01 00:00:00

  • In Situ Hydrogen Dynamics in a Hot Spring Microbial Mat during a Diel Cycle.

    abstract:UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00710-16

    authors: Revsbech NP,Trampe E,Lichtenberg M,Ward DM,Kühl M

    更新日期:2016-06-30 00:00:00

  • Expression of a novel small antimicrobial protein from the seeds of motherwort (Leonurus japonicus) confers disease resistance in tobacco.

    abstract::Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02016-06

    authors: Yang X,Xiao Y,Wang X,Pei Y

    更新日期:2007-02-01 00:00:00

  • Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp. strain SU.

    abstract::The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071, 1992). Genes fcbA and fcb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.2707-2711.2003

    authors: Zhuang Z,Gartemann KH,Eichenlaub R,Dunaway-Mariano D

    更新日期:2003-05-01 00:00:00

  • Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads.

    abstract::Suppression of soilborne disease by fluorescent pseudomonads may be inconsistent. Inefficient root colonization by the introduced bacteria is often responsible for this inconsistency. To better understand the bacterial traits involved in root colonization, the effect of two plant species, flax (Linum usitatissinum L.)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.3.1004-1012.1995

    authors: Lemanceau P,Corberand T,Gardan L,Latour X,Laguerre G,Boeufgras J,Alabouvette C

    更新日期:1995-03-01 00:00:00

  • Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water.

    abstract::A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.4.849-851.1980

    authors: Thorpe TC,Miller RD

    更新日期:1980-10-01 00:00:00

  • Improved specimen coating technique for scanning electron microscope observation of decomposer microorganisms.

    abstract::Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.2.313-315.1976

    authors: Draggan S

    更新日期:1976-02-01 00:00:00