Novel polyketide synthase from Nectria haematococca.

Abstract:

:We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase, duplicated acyl carrier proteins, and thioesterase. The pksN product groups with the Aspergillus nidulans WA-type PKSs involved in conidial pigmentation and melanin, bikaverin, and aflatoxin biosynthetic pathways. Inactivation of pksN did not cause any visible change in fungal growth, asexual sporulation, or ascospore formation, suggesting that it is involved in a specific developmental function. We propose that pksN encodes a novel PKS required for the perithecial red pigment biosynthesis.

journal_name

Appl Environ Microbiol

authors

Graziani S,Vasnier C,Daboussi MJ

doi

10.1128/aem.70.5.2984-2988.2004

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

2984-8

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

70

pub_type

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