Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in soil by quantitative reverse transcription-PCR.

abstract：:Relationships between the hyphae of Agaricus bisporus (Lang) Sing and bacteria from the mushroom bed casing layer were examined with a scanning electron microscope. Hyphae growing in the casing layer differed morphologically from compost-grown hyphae. Whereas the compost contained thin single hyphae surrounded by calc...

journal_title：Applied and environmental microbiology

authors： Masaphy S,Levanon D,Tchelet R,Henis Y

更新日期：1987-05-01 00:00:00

abstract：:Previous studies from this laboratory have documented the presence of coliform bacteria emanating from wooden reservoirs containing finished drinking water. Coliforms were identified as Klebsiella pneumoniae and Enterobacter spp. In the present report, evidence is presented which suggests that the origin of these coli...

journal_title：Applied and environmental microbiology

authors： Bagley ST,Seidler RJ,Talbot HW Jr,Morrow JE

更新日期：1978-07-01 00:00:00

abstract：:The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

journal_title：Applied and environmental microbiology

authors： Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

更新日期：2011-08-15 00:00:00

abstract：:The multistep cleavage of carotenoids in Mucorales during the sexual phase results in a cocktail of trisporic acid (C18) sex pheromones. We hypothesized that the C18 trisporoid intermediates have a specific regulatory function for sex pheromone production and carotenogenesis that varies with genus/species and vegetati...

journal_title：Applied and environmental microbiology

authors： Sahadevan Y,Richter-Fecken M,Kaerger K,Voigt K,Boland W

更新日期：2013-12-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for ...

journal_title：Applied and environmental microbiology

authors： Rayamajhi N,Cha SB,Kang ML,Lee SI,Lee HS,Yoo HS

更新日期：2009-09-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:Shellfish-transmitted Vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the Northeast United States. This change coincided with a bacterial population shift toward human-pathogenic variants occurring in part through the introduction of several Pa...

journal_title：Applied and environmental microbiology

authors： Xu F,Gonzalez-Escalona N,Drees KP,Sebra RP,Cooper VS,Jones SH,Whistler CA

更新日期：2017-08-31 00:00:00

abstract：:Pure cultures of methylotrophs and methanotrophs are known to oxidize methyl bromide (MeBr); however, their ability to oxidize tropospheric concentrations (parts per trillion by volume [pptv]) has not been tested. Methylotrophs and methanotrophs were able to consume MeBr provided at levels that mimicked the tropospher...

journal_title：Applied and environmental microbiology

authors： Goodwin KD,Varner RK,Crill PM,Oremland RS

更新日期：2001-12-01 00:00:00

abstract：:Newcastle disease, one of the most important health problems that affects the poultry industry around the world, is caused by virulent strains of Newcastle disease virus. Newcastle disease virus is considered to be endemic in several countries in the Americas, including Mexico. In order to control Newcastle disease ou...

journal_title：Applied and environmental microbiology

authors： Cardenas Garcia S,Navarro Lopez R,Morales R,Olvera MA,Marquez MA,Merino R,Miller PJ,Afonso CL

更新日期：2013-08-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:The human intestine harbors both lactate-producing and lactate-utilizing bacteria. Lactate is normally present at <3 mmol liter(-1) in stool samples from healthy adults, but concentrations up to 100 mmol liter(-1) have been reported in gut disorders such as ulcerative colitis. The effect of different initial pH values...

journal_title：Applied and environmental microbiology

authors： Belenguer A,Duncan SH,Holtrop G,Anderson SE,Lobley GE,Flint HJ

更新日期：2007-10-01 00:00:00

abstract：:Several anaerobic bacteria isolated from the sediments of Contrary Creek, an iron-rich environment, produced magnetite when cultured in combinations but not when cultured alone in synthetic iron oxyhydroxide medium. When glucose was added as a carbon source, the pH of the medium decreased (to 5.5) and no magnetite was...

journal_title：Applied and environmental microbiology

authors： Bell PE,Mills AL,Herman JS

更新日期：1987-11-01 00:00:00

abstract：:Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO

更新日期：1998-05-01 00:00:00

abstract：:Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

journal_title：Applied and environmental microbiology

authors： Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

更新日期：1992-12-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus can grow with short- to long-chain fatty acids as the sole carbon source (R. G. Kranz, K. K. Gabbert, T. A. Locke, and M. T. Madigan, Appl. Environ. Microbiol. 63:3003-3009, 1997). Concomitant with growth on fatty acids is the production to high levels of the polyest...

journal_title：Applied and environmental microbiology

authors： Kranz RG,Gabbert KK,Madigan MT

更新日期：1997-08-01 00:00:00

abstract：:To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...

journal_title：Applied and environmental microbiology

authors： Nelson WC,Maezato Y,Wu YW,Romine MF,Lindemann SR

更新日期：2015-10-23 00:00:00

abstract：:Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

journal_title：Applied and environmental microbiology

authors： Chu FS,Huang X,Wei RD,Carmichael WW

更新日期：1989-08-01 00:00:00

abstract：:Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from sta...

journal_title：Applied and environmental microbiology

authors： Brown GS,Betty RG,Brockmann JE,Lucero DA,Souza CA,Walsh KS,Boucher RM,Tezak M,Wilson MC,Rudolph T

更新日期：2007-02-01 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00

abstract：:The inactivation of catechol 2,3-dioxygenase from Pseudomonas putida mt-2 by 3-chloro- and 3-fluorocatechol and the iron-chelating agent Tiron (catechol-3,5-disulfonate) was studied. Whereas inactivation by Tiron is an oxygen-independent and mostly reversible process, inactivation by the 3-halocatechols was only obser...

journal_title：Applied and environmental microbiology

authors： Bartels I,Knackmuss HJ,Reineke W

更新日期：1984-03-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00

abstract：:To examine the relationship between activated-sludge bulking and levels of specific filamentous bacteria, we developed a statistics-based quantification method for estimating the biomass levels of specific filaments using 16S rRNA-targeted fluorescent in situ hybridization (FISH) probes. The results of quantitative FI...

journal_title：Applied and environmental microbiology

authors： Liao J,Lou I,de los Reyes FL 3rd

更新日期：2004-04-01 00:00:00

abstract：:Gram-positive rubber degraders such as Streptomyces sp. strain K30 cleave rubber [poly(cis-1,4-isoprene)] to low-molecular-mass oligoisoprenoid products with terminal keto and aldehyde groups by the secretion of a latex clearing protein (Lcp) designated rubber oxygenase. LcpK30 is a heme b cytochrome and has a domain ...

journal_title：Applied and environmental microbiology

authors： Röther W,Austen S,Birke J,Jendrossek D

更新日期：2016-10-27 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00

abstract：:Identification and design of new cellulolytic enzymes with higher catalytic efficiency are a key factor in reducing the production cost of lignocellulosic bioalcohol. We report here identification of a novel β-glucosidase (Gluc1C) from Paenibacillus sp. strain MTCC 5639 and construction of bifunctional chimeric protei...

journal_title：Applied and environmental microbiology

authors： Adlakha N,Sawant S,Anil A,Lali A,Yazdani SS

更新日期：2012-10-01 00:00:00

abstract：:The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses, which are common in fresh water. This gene functions in vivo and also when cloned in Escherichia coli. Photodamaged viru...

journal_title：Applied and environmental microbiology

authors： Furuta M,Schrader JO,Schrader HS,Kokjohn TA,Nyaga S,McCullough AK,Lloyd RS,Burbank DE,Landstein D,Lane L,Van Etten JL

更新日期：1997-04-01 00:00:00