Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique.

abstract：:The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...

journal_title：Applied and environmental microbiology

authors： Phelps PA,Agarwal SK,Speitel GE,Georgiou G

更新日期：1992-11-01 00:00:00

abstract：:Free-living amoebae are frequent hosts for bacterial endosymbionts. In this study, the symbionts of eight novel environmental Acanthamoeba strains isolated from different locations worldwide were characterized. Phylogenetic analysis revealed that they were related to one of four evolutionary lineages of amoeba symbion...

journal_title：Applied and environmental microbiology

authors： Schmitz-Esser S,Toenshoff ER,Haider S,Heinz E,Hoenninger VM,Wagner M,Horn M

更新日期：2008-09-01 00:00:00

abstract：:The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as...

journal_title：Applied and environmental microbiology

authors： Van Dijck P,van de Voorde H

更新日期：1976-03-01 00:00:00

abstract：:Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

journal_title：Applied and environmental microbiology

authors： Bender J,Vatcharapijarn Y,Jeffries TW

更新日期：1985-03-01 00:00:00

abstract：:Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

journal_title：Applied and environmental microbiology

authors： Gilliland SE,Speck ML

更新日期：1977-01-01 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter up...

journal_title：Applied and environmental microbiology

authors： Soltes-Rak E,Kushner DJ,Williams DD,Coleman JR

更新日期：1993-08-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D(2)O at pD 6 by Fourier transform infrared spectroscopy....

journal_title：Applied and environmental microbiology

authors： Gaussier H,Lefèvre T,Subirade M

更新日期：2003-11-01 00:00:00

abstract：:The occurrence, structure, and mobility of Tn1546-like elements were studied in environmental vancomycin-resistant enterococci (VRE) isolated from municipal sewage, activated sludge, pharmaceutical waste derived from antibiotic production, seawater, blue mussels, and soil. Of 200 presumptive VRE isolates tested, 71 (3...

journal_title：Applied and environmental microbiology

authors： Guardabassi L,Dalsgaard A

更新日期：2004-02-01 00:00:00

abstract：:Bacillus cereus UW85 produces a novel aminopolyol antibiotic, zwittermicin A, that contributes to the ability of UW85 to suppress damping-off of alfalfa caused by Phytophthora medicaginis. UW85 produces a second antibiotic, provisionally designated antibiotic B, which also contributes to suppression of damping-off but...

journal_title：Applied and environmental microbiology

authors： Stabb EV,Jacobson LM,Handelsman J

更新日期：1994-12-01 00:00:00

abstract：:Pseudomonas aeruginosa JB2 was isolated from a polychlorinated biphenyl-contaminated soil by enrichment culture containing 2-chlorobenzoate as the sole carbon source. Strain JB2 was subsequently found also to grow on 3-chlorobenzoate, 2,3- and 2,5-dichlorobenzoates, 2,3,5-trichlorobenzoate, and a wide range of other m...

journal_title：Applied and environmental microbiology

authors： Hickey WJ,Focht DD

更新日期：1990-12-01 00:00:00

abstract：:Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplifie...

journal_title：Applied and environmental microbiology

authors： Gratacap-Cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM

更新日期：2000-06-01 00:00:00

abstract：:The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

journal_title：Applied and environmental microbiology

authors： Small FJ,Tilley JK,Ensign SA

更新日期：1995-04-01 00:00:00

abstract：:A mixed culture of nitrate-reducing bacteria degraded o-cresol in the presence of toulene as a primary growth substrate. No degradation of o-cresol was observed in the absence of toluene or when the culture grew on p-cresol and 2,4-dimethylphenol. In batch cultures, the degradation of o-cresol started after toluene wa...

journal_title：Applied and environmental microbiology

authors： Flyvbjerg J,Jørgensen C,Arvin E,Jensen BK,Olsen SK

更新日期：1993-07-01 00:00:00

abstract：:A mixture of a cell culture-adapted strain (C-486) of calf rotavirus and poliovirus type 1 (Sabin) was prepared in tryptose phosphate broth containing 0.1% uranine (physical tracer) and antifoam at a final concentration of 0.001%. By using a six-jet Collison nebulizer, the mixture was aerosolized into a 300-liter stai...

journal_title：Applied and environmental microbiology

authors： Ijaz MK,Sattar SA,Johnson-Lussenburg CM,Springthorpe VS

更新日期：1985-02-01 00:00:00

abstract：:In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Amaro C

更新日期：1996-07-01 00:00:00

abstract：:Salmonella enterica contamination in foods is a significant concern for public health. When DNA detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assa...

journal_title：Applied and environmental microbiology

authors： González-Escalona N,Hammack TS,Russell M,Jacobson AP,De Jesús AJ,Brown EW,Lampel KA

更新日期：2009-06-01 00:00:00

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:Sludges derived from wastewater treatment are foul-smelling, biologically unstable substances. As well as containing numerous pathogenic microorganisms, they also consist of organic matter that can be used as agricultural fertilizer. Legislation nevertheless requires sludges to be virologically tested prior to spreadi...

journal_title：Applied and environmental microbiology

authors： Monpoeho S,Maul A,Bonnin C,Patria L,Ranarijaona S,Billaudel S,Ferré V

更新日期：2004-09-01 00:00:00

abstract：:Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

journal_title：Applied and environmental microbiology

authors： Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

更新日期：2002-10-01 00:00:00

abstract：:Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a deri...

journal_title：Applied and environmental microbiology

authors： Villacieros M,Whelan C,Mackova M,Molgaard J,Sánchez-Contreras M,Lloret J,Aguirre de Cárcer D,Oruezábal RI,Bolaños L,Macek T,Karlson U,Dowling DN,Martín M,Rivilla R

更新日期：2005-05-01 00:00:00

abstract：:Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal ...

journal_title：Applied and environmental microbiology

authors： Moore WE,Moore LV,Cato EP,Wilkins TD,Kornegay ET

更新日期：1987-07-01 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic ac...

journal_title：Applied and environmental microbiology

authors： Lee-Wickner LJ,Chassy BM

更新日期：1984-11-01 00:00:00

abstract：:Several variations in the scintillation mixture and the filter paper arrangements for double-vial radiorespirometry were compared. Improved efficiencies (44%) and shorter response times were found by adding wetting agents and methanolic NaOH to the scintillation mixture in the filter paper. The scintillation chemicals...

journal_title：Applied and environmental microbiology

authors： McKinley VL,Federle TW,Vestal JR

更新日期：1983-01-01 00:00:00

abstract：:Wastewater management in the Canadian Arctic is challenging due to climate extremes, small population sizes, and lack of conventional infrastructure for wastewater treatment. Although many Northern communities use waste stabilization ponds (WSPs) as their primary form of wastewater treatment, few studies have explored...

journal_title：Applied and environmental microbiology

authors： Gromala M,Neufeld JD,McConkey BJ

更新日期：2021-01-15 00:00:00

abstract：:A basal (BC) medium devoid of added carbohydrates, a complete (CC) medium containing nine carbohydrates were developed for enumerating rumen bacteria. The colony counts on the BC medium were 85 to 100% of those obtained on the CC medium. These colonies were pinpoint size (less than or equal to mm in diameter) but incr...

journal_title：Applied and environmental microbiology

authors： Leedle JA,Hespell RB

更新日期：1980-04-01 00:00:00

abstract：:Anaerobic incubation of membrane filter cultures significantly enhanced detection of fecal coliforms in surface-water samples from recreational beaches. In contrast to standard aerobic incubation, anaerobic incubation suppressed overgrowth of masking, noncoliform bacteria but did not increase the frequency of fecal co...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Brickler SK,Kramer RE,Sinclair NA

更新日期：1984-08-01 00:00:00

abstract：:Here, we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., M...

journal_title：Applied and environmental microbiology

authors： Eardly B,Elia P,Brockwell J,Golemboski D,van Berkum P

更新日期：2017-05-01 00:00:00