Production and Regeneration of Lactobacillus casei Protoplasts.

abstract：:Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of th...

journal_title：Applied and environmental microbiology

authors： Fassel TA,Buchholz LA,Collins ML,Remsen CC

更新日期：1992-07-01 00:00:00

abstract：:A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

journal_title：Applied and environmental microbiology

authors： Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

更新日期：1995-12-01 00:00:00

abstract：:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the ...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG

更新日期：1996-02-01 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcesce...

journal_title：Applied and environmental microbiology

authors： Hover T,Maya T,Ron S,Sandovsky H,Shadkchan Y,Kijner N,Mitiagin Y,Fichtman B,Harel A,Shanks RM,Bruna RE,García-Véscovi E,Osherov N

更新日期：2016-04-18 00:00:00

abstract：:Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sedimen...

journal_title：Applied and environmental microbiology

authors： Brad T,Braster M,van Breukelen BM,van Straalen NM,Röling WF

更新日期：2008-07-01 00:00:00

abstract：:Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

journal_title：Applied and environmental microbiology

authors： Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

更新日期：1990-04-01 00:00:00

abstract：:The natural abundance of stable carbon isotopes measured in bacterial nucleic acids extracted from estuarine bacterial concentrates was used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of Pseudomonas aeruginosa and nucleic acids extracted from cultures rese...

journal_title：Applied and environmental microbiology

authors： Coffin RB,Velinsky DJ,Devereux R,Price WA,Cifuentes LA

更新日期：1990-07-01 00:00:00

abstract：:Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...

journal_title：Applied and environmental microbiology

authors： Waterfield N,Dowling A,Sharma S,Daborn PJ,Potter U,Ffrench-Constant RH

更新日期：2001-11-01 00:00:00

abstract：UNLABELLED:Salmonella is recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial s...

journal_title：Applied and environmental microbiology

authors： Karampoula F,Giaouris E,Deschamps J,Doulgeraki AI,Nychas GJ,Dubois-Brissonnet F

更新日期：2016-08-15 00:00:00

abstract：:Population sizes of algae, bacteria, heterotrophic flagellates, and viruses were observed through the 1989 spring diatom bloom in Raunefjorden in western Norway. The culmination of the diatom bloom was followed by a peak in the concentration of bacteria and an increase in the concentration of heterotrophic flagellates...

journal_title：Applied and environmental microbiology

authors： Bratbak G,Heldal M,Norland S,Thingstad TF

更新日期：1990-05-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...

journal_title：Applied and environmental microbiology

authors： Robinson IM,Whipp SC,Bucklin JA,Allison MJ

更新日期：1984-11-01 00:00:00

abstract：:The incidence of microbes in the nasal cavities of workers in three paper and board mills was investigated. A total of 234 persons exposed to microbial aerosols and splashes from paper machine wires and debarker drums formed the exposed group. The control group consisted of 294 workers from the dry working areas: the ...

journal_title：Applied and environmental microbiology

authors： Niemelä SI,Väätänen P,Mentu J,Jokinen A,Jäppinen P,Sillanpää P

更新日期：1985-07-01 00:00:00

abstract：:Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...

journal_title：Applied and environmental microbiology

authors： Cohen-Rosenzweig C,Guan Z,Shaanan B,Eichler J

更新日期：2014-01-01 00:00:00

abstract：:Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

journal_title：Applied and environmental microbiology

authors： Jian H,Xiao X,Wang F

更新日期：2013-11-01 00:00:00

abstract：:Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...

journal_title：Applied and environmental microbiology

authors： McMillan DG,Keis S,Berney M,Cook GM

更新日期：2009-12-01 00:00:00

abstract：:Lactobacillus bulgaricus LT4(0448) is a lysogenic strain from which a temperate bacteriophage can be induced by mitomycin C or UV irradiation. Lactobacillus lactis CNRZ 326 is an indicator strain for the temperate phage 0448, but this strain lyses only in the presence of Ca ions. A resistant culture developed secondar...

journal_title：Applied and environmental microbiology

authors： Cluzel PJ,Serio J,Accolas JP

更新日期：1987-08-01 00:00:00

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

journal_title：Applied and environmental microbiology

authors： Qualls RG,Johnson JD

更新日期：1983-03-01 00:00:00

abstract：:Leghorn hens over 50 weeks of age were assigned to two treatment groups designated as either unmolted controls or molted. A forced molt was induced by a 9-day feed withdrawal, and each hen was challenged orally with 10(5) Salmonella enteritidis organisms on day 4 of feed withdrawal. On days 4 and 9 of molt, the number...

journal_title：Applied and environmental microbiology

authors： Durant JA,Corrier DE,Byrd JA,Stanker LH,Ricke SC

更新日期：1999-05-01 00:00:00

abstract：:Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge during a period of adaptation to phenol, only an antiserum against one of the isolates, Alcaligenes sp. E2, reacted with the ...

journal_title：Applied and environmental microbiology

authors： Watanabe K,Hino S

更新日期：1996-10-01 00:00:00

abstract：:Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus f...

journal_title：Applied and environmental microbiology

authors： Solheim M,Aakra A,Vebø H,Snipen L,Nes IF

更新日期：2007-09-01 00:00:00

abstract：:The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

journal_title：Applied and environmental microbiology

authors： Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

更新日期：2011-04-01 00:00:00

abstract：:Dehalobacter and "Dehalococcoides" spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a Dehalobacter sp. from this mixed...

journal_title：Applied and environmental microbiology

authors： Grostern A,Edwards EA

更新日期：2009-05-01 00:00:00

abstract：:Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

journal_title：Applied and environmental microbiology

authors： Khush RS,Becker E,Wach M

更新日期：1992-09-01 00:00:00

abstract：:Interactions between photoautotrophic and heterotrophic microorganisms are central to the marine microbial ecosystem. Lab cultures of one of the dominant marine photoautotrophs, Synechococcus, have historically been difficult to render axenic, presumably because these bacteria depend upon other organisms to grow under...

journal_title：Applied and environmental microbiology

authors： Zheng Q,Wang Y,Xie R,Lang AS,Liu Y,Lu J,Zhang X,Sun J,Suttle CA,Jiao N

更新日期：2018-01-17 00:00:00

abstract：:Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...

journal_title：Applied and environmental microbiology

authors： Fuentes FA,Biamon EJ,Hazen TC

更新日期：1983-12-01 00:00:00

abstract：:Ceftiofur, a third-generation cephalosporin antimicrobial, was used in Canadian hatcheries for many years to prevent early mortality in chicks, leading to a high prevalence of cephalosporin resistance in Escherichia coli in chickens. Preventive use of ceftiofur in hatcheries ceased in 2014. We examined the effect of c...

journal_title：Applied and environmental microbiology

authors： Verrette L,Fairbrother JM,Boulianne M

更新日期：2019-06-17 00:00:00

abstract：:The anti-norovirus (anti-NoV) effect of grape seed extract (GSE) was examined by plaque assay for murine norovirus 1 (MNV-1), cell-binding reverse transcription-PCR for human NoV GII.4, and saliva-binding enzyme-linked immunosorbent assay for human NoV GII.4 P particles, with or without the presence of interfering sub...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,Zhang D,Xia M,Zhong W,Van Coillie E,Jiang X,Uyttendaele M

更新日期：2012-11-01 00:00:00