Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWW0 under various conditions of nutrient limitation in chemostat culture.


:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the TOL pathway by succinate in chemostat cultures run at a low dilution rate (D = 0.05 h-1) under different conditions of inorganic-nutrient limitation. The activity of benzylalcohol dehydrogenase (BADH) in cell extracts was used as a measure of the expression of the TOL upper pathway. When cells were grown in the presence of 10 to 15 mM succinate under conditions of phosphate or sulfate limitation, the BADH activity in response to the nonmetabolizable inducer o-xylene was less than 2% of that of cells grown under conditions of succinate limitation. Less repression was found under conditions of ammonium or oxygen limitation (2 to 10% and 20 to 35%, respectively, of the BADH levels under succinate limitation). The BADH expression levels determined under the different growth conditions appeared to correlate well with the mRNA transcript levels from the upper pathway promoter (Pu), which indicates that repression was due to a blockage at the transcriptional level. The meta-cleavage pathway was found to be less susceptible to catabolite repression. The results obtained suggest that the occurrence of catabolite repression is related to a high-energy status of the cells rather than to a high growth rate or directly to the presence of growth-saturating concentrations of a primary carbon and energy source.


Appl Environ Microbiol


Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG




Has Abstract


1996-02-01 00:00:00












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    abstract::T1, a dentrifying bacterium originally isolated for its ability to grow on toluene, can also metabolize 4-hydroxybenzoate and other aromatic compounds under denitrifying conditions. A cosmid clone carrying the three genes that code for the 4-chlorobenzoate dehalogenase enzyme complex isolated from the aerobic bacteriu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Coschigano PW,Häggblom MM,Young LY

    更新日期:1994-03-01 00:00:00

  • PhaG-mediated synthesis of Poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.

    abstract::Recently, a new metabolic link between fatty acid de novo biosynthesis and biosynthesis of poly(3-hydroxy-alkanoate) consisting of medium-chain-length constituents (C(6) to C(14)) (PHA(MCL)), catalyzed by the 3-hydroxydecanoyl-[acyl-carrier-protein]:CoA transacylase (PhaG), has been identified in Pseudomonas putida (B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fiedler S,Steinbüchel A,Rehm BH

    更新日期:2000-05-01 00:00:00

  • Novel Rickettsiella bacterium in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae).

    abstract::Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Iasur-Kruh L,Weintraub PG,Mozes-Daube N,Robinson WE,Perlman SJ,Zchori-Fein E

    更新日期:2013-07-01 00:00:00

  • Isolation of a bacterial strain with the ability to utilize the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy.

    abstract::A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy was isolated. This strain was obtained by continuous adaptation of "Hydrogenophaga palleronii" S1, which has the ability to grow aerobically with 4-aminobenzenesu...

    journal_title:Applied and environmental microbiology

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    authors: Blümel S,Contzen M,Lutz M,Stolz A,Knackmuss HJ

    更新日期:1998-06-01 00:00:00

  • Genetic Diversity of Xanthomonas oryzae pv. oryzae in Asia.

    abstract::Restriction fragment length polymorphism and virulence analyses were used to evaluate the population structure of Xanthomonas oryzae pv. oryzae, the rice bacterial blight pathogen, from several rice-growing countries in Asia. Two DNA sequences from X. oryzae pv. oryzae, IS1112, an insertion sequence, and avrXa10, a me...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


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  • Reductive dechlorination of chlorinated ethenes and 1, 2-dichloroethane by "Dehalococcoides ethenogenes" 195.

    abstract::"Dehalococcoides ethenogenes" 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol/vol] inoculum) into growth medium amended with trichloroethene (TCE), cis-dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron accep...

    journal_title:Applied and environmental microbiology

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    authors: Maymó-Gatell X,Anguish T,Zinder SH

    更新日期:1999-07-01 00:00:00

  • Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Härtig C,Loffhagen N,Harms H

    更新日期:2005-04-01 00:00:00

  • 3-Hydroxypyridine Dehydrogenase HpdA Is Encoded by a Novel Four-Component Gene Cluster and Catalyzes the First Step of 3-Hydroxypyridine Catabolism in Ensifer adhaerens HP1.

    abstract::3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as its sole sources of carbon, nitrogen, and energy to grow, but the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, might be respons...

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    pub_type: 杂志文章


    authors: Wang H,Wang X,Ren H,Wang X,Lu Z

    更新日期:2020-09-17 00:00:00

  • Ruminal Degradation of Rumen-Protected Glucose Influences the Ruminal Microbiota and Metabolites in Early-Lactation Dairy Cows.

    abstract::Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-...

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    authors: Wang Y,Nan X,Zhao Y,Wang Y,Jiang L,Xiong B

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  • Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.

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    authors: Lewicka E,Dolowy P,Godziszewska J,Litwin E,Ludwiczak M,Jagura-Burdzy G

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    authors: Peiro C,Millard P,de Simone A,Cahoreau E,Peyriga L,Enjalbert B,Heux S

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    authors: Compant S,Reiter B,Sessitsch A,Nowak J,Clément C,Ait Barka E

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  • Biosynthesis of Poly-beta-Hydroxyalkanoates from Pentoses by Pseudomonas pseudoflava.

    abstract::The potential of Pseudomonas pseudoflava to produce poly-beta-hydroxyalkanoates (PHAs) from pentoses was studied. This organism was able to use a hydrolysate from the hemicellulosic fraction of poplar wood as a carbon and energy source for its growth. However, in batch cultures, growth was inhibited completely at hydr...

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    pub_type: 杂志文章


    authors: Bertrand JL,Ramsay BA,Ramsay JA,Chavarie C

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    abstract::An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

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    authors: Coleman NV,Spain JC

    更新日期:2003-10-01 00:00:00

  • The plasmid-encoded lactococcal envelope-associated proteinase is encoded by a chromosomal gene in Lactococcus lactis subsp. cremoris BC101.

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    authors: Nissen-Meyer J,Lillehaug D,Nes IF

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    abstract::Laboratory-grown strains of chemoautotrophic Thiomicrospira sp. strain L-12 and Thiobacillus neapolitanus produced cell carbon that was 24.6 to 25.1 ppt (24.6 to 25.1 mg/g) lower in C isotope abundance than the ambient source of carbon dioxide and bicarbonate. This degree of C isotope depletion was comparable to that ...

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    authors: Ruby EG,Jannasch HW,Deuser WG

    更新日期:1987-08-01 00:00:00

  • Degradation of pyrene, benz[a]anthracene, and benzo[a]pyrene by Mycobacterium sp. strain RJGII-135, isolated from a former coal gasification site.

    abstract::The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tani K,Kurokawa K,Nasu M

    更新日期:1998-04-01 00:00:00

  • Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens.

    abstract:UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Doyle MP

    更新日期:1984-03-01 00:00:00

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    abstract::Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

    更新日期:1980-12-01 00:00:00

  • Proposals for optimization of the international phage typing system for Listeria monocytogenes: combined analysis of phage lytic spectrum and variability of typing results.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Marquet-Van der Mee N,Audurier A

    更新日期:1995-01-01 00:00:00

  • Incidence and properties of temperate bacteriophages induced from lactic streptococci.

    abstract::Sixty-three strains of lactic streptococci isolated from commercial lactic streptococcal starter cultures were examined for lysogeny by treatment with ultraviolet light or mitomycin C. After treatment with the inducing agent, all strains, whether or not they lysed, were examined for evidence of phage release by electr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Huggins AR,Sandine WE

    更新日期:1977-01-01 00:00:00

  • Coastal strains of marine Synechococcus species exhibit increased tolerance to copper shock and a distinctive transcriptional response relative to those of open-ocean strains.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

    更新日期:2009-08-01 00:00:00

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Merkal RS,Lyle PS,Whipple DL

    更新日期:1981-06-01 00:00:00

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Viana T,Loureiro-Dias MC,Loureiro V,Prista C

    更新日期:2012-09-01 00:00:00