Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate.

Abstract:

:Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus faecalis V583 by traditional growth studies and microarrays. Transcriptional responses were studied by time course experiments. In the presence of BB (V583-BB) or SDS (V583-SDS), 308 and 209 genes were identified as differentially expressed at one or more time points, respectively. In V583 treated with both BB and SDS (V583-BB-SDS), 254 genes showed differential expression. Detergents exert their toxic effects primarily on the microbial membrane. The enrichment of differentially transcribed genes that encode proteins with membrane-associated functions and/or locations indicates a major impact of all three treatments on the integrity and functionality of the cell membrane. Two gene clusters involved in fatty acid biosynthesis were repressed in V583-BB and V583-BB-SDS and partly induced in V583-SDS. Furthermore, two EmrB/QacA family drug resistance transporters and a vacuolar-type ATPase were induced in V583-BB and V583-BB-SDS. None of the putative bile salt hydrolase homologs in V583 showed differential expression during the bile treatments. The transcriptional profile of V583-BB-SDS was qualitatively more similar to the response in V583-BB than to that in V583-SDS, suggesting that the presence of bile suppresses the effects of SDS in V583-BB-SDS. The overall results presented here indicate that different mechanisms are involved in detergent resistance in E. faecalis.

journal_name

Appl Environ Microbiol

authors

Solheim M,Aakra A,Vebø H,Snipen L,Nes IF

doi

10.1128/AEM.00651-07

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

5767-74

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.00651-07

journal_volume

73

pub_type

杂志文章
  • Identification of bacterial species associated with the sheep scab mite (Psoroptes ovis) by using amplified genes coding for 16S rRNA.

    abstract::This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4227-4229.1999

    authors: Hogg JC,Lehane MJ

    更新日期:1999-09-01 00:00:00

  • Recovery and phylogenetic analysis of nifH sequences from diazotrophic bacteria associated with dead aboveground biomass of Spartina alterniflora.

    abstract::DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5308-5314.2001

    authors: Lovell CR,Friez MJ,Longshore JW,Bagwell CE

    更新日期:2001-11-01 00:00:00

  • Biphasic culture system for rapid Campylobacter cultivation.

    abstract::We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.1.284-289.1983

    authors: Rollins DM,Coolbaugh JC,Walker RI,Weiss E

    更新日期:1983-01-01 00:00:00

  • Entianin, a novel subtilin-like lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with high antimicrobial activity.

    abstract::Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01962-10

    authors: Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

    更新日期:2011-03-01 00:00:00

  • Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community genome amplification.

    abstract::Microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. We developed a whole-community genome amplification-assisted microarray detection approac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02738-05

    authors: Wu L,Liu X,Schadt CW,Zhou J

    更新日期:2006-07-01 00:00:00

  • Multispecies Diesel Fuel Biodegradation and Niche Formation Are Ignited by Pioneer Hydrocarbon-Utilizing Proteobacteria in a Soil Bacterial Consortium.

    abstract::A soil bacterial consortium that was grown on diesel fuel and consisted of more than 10 members from different genera was maintained through repetitive subculturing and was utilized as a practical model to investigate a bacterial community that was continuously exposed to petroleum hydrocarbons. Through metagenomics a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02268-20

    authors: Mori JF,Kanaly RA

    更新日期:2020-12-17 00:00:00

  • Relationship between nitrogen-fixing sulfate reducers and fermenters in salt marsh sediments and roots of Spartina alterniflora.

    abstract::A combination of inhibitors and carbon substrates was used to determine the relative contribution of sulfate-reducing bacteria (SRB) and fermenting bacteria to nitrogen fixation in a salt marsh sediment and on the roots of Spartina alterniflora. Because a lag period precedes acetylene-reducing activity (ARA) in amende...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.8.2031-2036.1988

    authors: Gandy EL,Yoch DC

    更新日期:1988-08-01 00:00:00

  • Accumulation and fate of microorganisms and microspheres in biofilms formed in a pilot-scale water distribution system.

    abstract::The accumulation and fate of model microbial "pathogens" within a drinking-water distribution system was investigated in naturally grown biofilms formed in a novel pilot-scale water distribution system provided with chlorinated and UV-treated water. Biofilms were exposed to 1-mum hydrophilic and hydrophobic microspher...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.2.706-712.2005

    authors: Långmark J,Storey MV,Ashbolt NJ,Stenström TA

    更新日期:2005-02-01 00:00:00

  • Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences.

    abstract::The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.6.1379-1384.1985

    authors: Stahl DA,Lane DJ,Olsen GJ,Pace NR

    更新日期:1985-06-01 00:00:00

  • Assessment of factors influencing direct enumeration of viruses within estuarine sediments.

    abstract::Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00297-06

    authors: Helton RR,Liu L,Wommack KE

    更新日期:2006-07-01 00:00:00

  • Characterization of a Nuclear Polyhedrosis Virus Isolated from Diseased Gonometa podocarpi (Lepidoptera:Lasiocampidae).

    abstract::Gonometa podocarpi is an important pest of several species of pine in East Africa, and large numbers of trees in plantations in Kenya were partially or completely defoliated by the larval stage of this insect. After the infestation in the Mt. Elgon region, large numbers of dead and moribund larvae were found on the gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.2.308-316.1981

    authors: Moore NF,Armstrong T,Green B,Brown D,Hibbin J,Kelly DC,Tinsley TW,Aloo TC

    更新日期:1981-08-01 00:00:00

  • Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998-1999 outbreak.

    abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02056-10

    authors: Elhanafi D,Dutta V,Kathariou S

    更新日期:2010-12-01 00:00:00

  • A New Player in the Biorefineries Field: Phasin PhaP Enhances Tolerance to Solvents and Boosts Ethanol and 1,3-Propanediol Synthesis in Escherichia coli.

    abstract::The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granule-associated protein that protects recombinant Escherichia coli against several kinds of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00662-17

    authors: Mezzina MP,Álvarez DS,Egoburo DE,Díaz Peña R,Nikel PI,Pettinari MJ

    更新日期:2017-06-30 00:00:00

  • Production and Characterization of Trametes versicolor Mutants Unable To Bleach Hardwood Kraft Pulp.

    abstract::Protoplasts of the monokaryotic strain 52J of Trametes versicolor were treated with UV light and screened for the inability to produce a colored precipitate on guaiacol-containing agar plates. Mutants unable to oxidize guaiacol had absent or very low secretion of laccase and manganese peroxidase (MnP) proteins. All is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.10.3687-3694.1995

    authors: Addleman K,Dumonceaux T,Paice MG,Bourbonnais R,Archibald FS

    更新日期:1995-10-01 00:00:00

  • Pathogenic Aeromonas hydrophila serogroup O:14 and O:81 strains with an S layer.

    abstract::Five autoagglutinating Aeromonas hydrophila isolates recovered from eels and humans were assigned to serogroups O:14 and O:81 of the Sakazaki and Shimada (National Institutes of Health) scheme. They had the following properties in common: positive precipitation after boiling, moderate surface hydrophobicity (salt-aggr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.5898-5904.2004

    authors: Esteve C,Alcaide E,Canals R,Merino S,Blasco D,Figueras MJ,Tomás JM

    更新日期:2004-10-01 00:00:00

  • Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure.

    abstract::Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reducti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.2243-2247.2000

    authors: Woo IS,Rhee IK,Park HD

    更新日期:2000-05-01 00:00:00

  • Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice.

    abstract::Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02841-08

    authors: Bergholz TM,Vanaja SK,Whittam TS

    更新日期:2009-06-01 00:00:00

  • Biochemical and genetic characterization of the Enterococcus faecalis oxaloacetate decarboxylase complex.

    abstract::Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03980-12

    authors: Repizo GD,Blancato VS,Mortera P,Lolkema JS,Magni C

    更新日期:2013-05-01 00:00:00

  • Sulfur isotope enrichment during maintenance metabolism in the thermophilic sulfate-reducing bacterium Desulfotomaculum putei.

    abstract::Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02948-08

    authors: Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

    更新日期:2009-09-01 00:00:00

  • Isolation and Properties of Mutants of Zymomonas mobilis Deficient in Sugar Assimilation.

    abstract::An enrichment method using d-cycloserine was designed for the isolation of spontaneous mutants of Zymomonas mobilis deficient in glucose or fructose utilization. The mutants could easily be isolated since they represented 80 to 90% of the population after two and three enrichment cycles. Glucokinase and fructokinase a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.3.1096-1098.1996

    authors: Ait-Abdelkader N,Pencreach G,Joset F,Baratti JC

    更新日期:1996-03-01 00:00:00

  • Persistence and leaching potential of microorganisms and mineral N in animal manure applied to intact soil columns.

    abstract::Pathogens may reach agricultural soils through application of animal manure and thereby pose a risk of contaminating crops as well as surface and groundwater. Treatment and handling of manure for improved nutrient and odor management may also influence the amount and fate of manure-borne pathogens in the soil. A study...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02506-12

    authors: Amin MG,Forslund A,Bui XT,Juhler RK,Petersen SO,Lægdsmand M

    更新日期:2013-01-01 00:00:00

  • Evidence of the internalization of animal caliciviruses via the roots of growing strawberry plants and dissemination to the fruit.

    abstract::Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03867-14

    authors: DiCaprio E,Culbertson D,Li J

    更新日期:2015-04-01 00:00:00

  • Modeling yeast spoilage in cold-filled ready-to-drink beverages with Saccharomyces cerevisiae, Zygosaccharomyces bailii, and Candida lipolytica.

    abstract::Mathematical models were developed to predict the probability of yeast spoilage of cold-filled ready-to-drink beverages as a function of beverage formulation. A Box-Behnken experimental design included five variables, each at three levels: pH (2.8, 3.3, and 3.8), titratable acidity (0.20, 0.40, and 0.60%), sugar conte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1901-1906.2002

    authors: Battey AS,Duffy S,Schaffner DW

    更新日期:2002-04-01 00:00:00

  • Mapping the Neutralizing Epitopes of Enterotoxigenic Escherichia coli K88 (F4) Fimbrial Adhesin and Major Subunit FaeG.

    abstract::Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effectiv...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00329-19

    authors: Lu T,Moxley RA,Zhang W

    更新日期:2019-05-16 00:00:00

  • An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.

    abstract::An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02668-06

    authors: Mihasan M,Chiribau CB,Friedrich T,Artenie V,Brandsch R

    更新日期:2007-04-01 00:00:00

  • Ultrastructural and Single-Cell-Level Characterization Reveals Metabolic Versatility in a Microbial Eukaryote Community from an Ice-Covered Antarctic Lake.

    abstract:UNLABELLED:The McMurdo Dry Valleys (MCM) of southern Victoria Land, Antarctica, harbor numerous ice-covered bodies of water that provide year-round liquid water oases for isolated food webs dominated by the microbial loop. Single-cell microbial eukaryotes (protists) occupy major trophic positions within this truncated ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00478-16

    authors: Li W,Podar M,Morgan-Kiss RM

    更新日期:2016-05-31 00:00:00

  • Antimicrobial use and resistance in swine waste treatment systems.

    abstract::Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm sa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01087-06

    authors: Jindal A,Kocherginskaya S,Mehboob A,Robert M,Mackie RI,Raskin L,Zilles JL

    更新日期:2006-12-01 00:00:00

  • Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells.

    abstract::Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. For this reason, another means of detecting this bacterium is necessary. In the pre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2651-2655.1991

    authors: Brauns LA,Hudson MC,Oliver JD

    更新日期:1991-09-01 00:00:00

  • Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models.

    abstract::Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02609-15

    authors: Tan MS,Rahman S,Dykes GA

    更新日期:2015-11-13 00:00:00

  • Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses.

    abstract::Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4200-4204.2003

    authors: Halpern M,Gancz H,Broza M,Kashi Y

    更新日期:2003-07-01 00:00:00