Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community genome amplification.

abstract：:Arhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the cla...

journal_title：Applied and environmental microbiology

authors： Dalvi S,Nicholson C,Najar F,Roe BA,Canaan P,Hartson SD,Fathepure BZ

更新日期：2014-11-01 00:00:00

abstract：:Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

更新日期：2015-12-18 00:00:00

abstract：:The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...

journal_title：Applied and environmental microbiology

authors： Gabler M,Fischer L

更新日期：1999-08-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：:A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glyci...

journal_title：Applied and environmental microbiology

authors： Juni E,Heym GA,Avery M

更新日期：1986-09-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:Endotoxin exposure occurs in homes and occupational environments and is known to cause adverse health effects. In order to compare results from different studies and establish standards, airborne endotoxin exposures should be assessed using standardized methods. Although the European Committee for Standardization (CEN...

journal_title：Applied and environmental microbiology

authors： Spaan S,Heederik DJ,Thorne PS,Wouters IM

更新日期：2007-10-01 00:00:00

abstract：:[This corrects the article on p. 507 in vol. 30.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-12-01 00:00:00

abstract：:Concentrations of airborne coliform bacteria as high as 1.5 X 10(4) m-3 were observed 8 cm above anaerobically digested sewage sludge applied to a forest clearcut. Dry conditions and high wind speeds tended to favor aerosol generation. ...

journal_title：Applied and environmental microbiology

authors： Edmonds RL,Littke W

更新日期：1978-12-01 00:00:00

abstract：:Rotavirus SA11, suspended in tryptose phosphate broth with 2.5 mg of rhodamine B per ml, was aerosolized (Collison nebulizer) into a rotating drum, and the aerosols were held at 20 +/- 1 degree C with the desired relative humidity (RH). An all-glass impinger with tryptose phosphate broth was used to collect 1-min (5.6...

journal_title：Applied and environmental microbiology

authors： Sattar SA,Ijaz MK,Johnson-Lussenburg CM,Springthorpe VS

更新日期：1984-04-01 00:00:00

abstract：:The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

journal_title：Applied and environmental microbiology

authors： Sasaki T,Ohshima Y

更新日期：1987-07-01 00:00:00

abstract：:The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

journal_title：Applied and environmental microbiology

authors： Kelso BHL,Smith RV,Laughlin RJ

更新日期：1999-01-01 00:00:00

abstract：:The ability of terminal restriction fragment (T-RFLP or TRF) profiles of 16S rRNA genes to provide useful information about the relative diversity of complex microbial communities was investigated by comparison with other methods. Four soil communities representing two pinyon rhizosphere and two between-tree (interspa...

journal_title：Applied and environmental microbiology

authors： Dunbar J,Ticknor LO,Kuske CR

更新日期：2000-07-01 00:00:00

abstract：:The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Borneman J,Triplett EW

更新日期：1998-12-01 00:00:00

abstract：:The susceptibility of a strain of Legionella pneumophila to disinfection by an organic halamine, free chlorine, and a mixture of the organic halamine and free chlorine was assessed. The organic halamine was found to have superior stability in solution and to exhibit adequate disinfectant potential over a period of 1 m...

journal_title：Applied and environmental microbiology

authors： Swango LJ,Wilt GR,Killen AD,Williams DE,Worley SD

更新日期：1987-12-01 00:00:00

abstract：:Commercial turkey flocks in North Carolina have been found to be colonized frequently with Campylobacter coli strains that are resistant to several antimicrobials (tetracycline, streptomycin, erythromycin, kanamycin, and ciprofloxacin/nalidixic acid). Such strains have been designated multidrug resistant (MDR). Howeve...

journal_title：Applied and environmental microbiology

authors： D'lima CB,Miller WG,Mandrell RE,Wright SL,Siletzky RM,Carver DK,Kathariou S

更新日期：2007-04-01 00:00:00

abstract：:Proteorhodopsin (PR), a photoactive proton pump containing retinal, is present in approximately half of all bacteria in the ocean, but its physiological role is still unclear, since very few strains carrying the PR gene have been cultured. The aim of this work was to characterize PR diversity in a North Sea water samp...

journal_title：Applied and environmental microbiology

authors： Riedel T,Tomasch J,Buchholz I,Jacobs J,Kollenberg M,Gerdts G,Wichels A,Brinkhoff T,Cypionka H,Wagner-Döbler I

更新日期：2010-05-01 00:00:00

abstract：:The lipopeptide, surfactin, is produced by Bacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam b...

journal_title：Applied and environmental microbiology

authors： Cooper DG,Macdonald CR,Duff SJ,Kosaric N

更新日期：1981-09-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Sporulation of Bacillus larvae NRRL B-3650 occurred only at aeration rates lower than those used for cultivation of most Bacillus species. One possible explanation for the requirement for a low level of aeration in B. larvae is that toxic forms of oxygen such as H(2)O(2) and superoxide are involved. The superoxide dis...

journal_title：Applied and environmental microbiology

authors： Dingman DW,Stahly DP

更新日期：1984-06-01 00:00:00

abstract：:A new compound, designated ML-449, structurally similar to the known 20-membered macrolactam BE-14106, was isolated from a marine sediment-derived Streptomyces sp. Cloning and sequencing of the 83-kb ML-449 biosynthetic gene cluster revealed its high level of similarity to the BE-14106 gene cluster. Comparison of the ...

journal_title：Applied and environmental microbiology

authors： Jørgensen H,Degnes KF,Dikiy A,Fjaervik E,Klinkenberg G,Zotchev SB

更新日期：2010-01-01 00:00:00

abstract：:While the contribution of Bacteria to bioremediation of oil-contaminated shorelines is well established, the response of Archaea to spilled oil and bioremediation treatments is unknown. The relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bio...

journal_title：Applied and environmental microbiology

authors： Röling WF,de Brito Couto IR,Swannell RP,Head IM

更新日期：2004-05-01 00:00:00

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (Triticum aestivum L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilize...

journal_title：Applied and environmental microbiology

authors： Coombs JT,Franco CM

更新日期：2003-09-01 00:00:00

abstract：:A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence...

journal_title：Applied and environmental microbiology

authors： Pichard SL,Paul JH

更新日期：1993-02-01 00:00:00

abstract：:Given the interest in Bacteroides species as microbial source tracking (MST) markers, and the limited knowledge of the survival of Bacteroides species in the environment, here we examine the survival of Bacteroides fragilis, B. thetaiotaomicron, and environmental species of Bacteroides by use of culture techniques and...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Blanch AR

更新日期：2010-11-01 00:00:00

abstract：:Secondary sewage effluent and renovated water from four wells at the Flushing Meadows Wastewater Renovation Project near Phoenix, Arizona, in operation since 1967, were assayed approximately every 2 months in 1974 for viruses and enteric bacteria during flooding periods. No viruses of Salmonella sp. were detected in a...

journal_title：Applied and environmental microbiology

authors： Gilbert RG,Gerba CP,Rice RC,Bouwer H,Wallis C,Melnick JL

更新日期：1976-09-01 00:00:00

abstract：:A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

journal_title：Applied and environmental microbiology

authors： Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

更新日期：2004-10-01 00:00:00

abstract：:Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns a...

journal_title：Applied and environmental microbiology

authors： Mangin I,Bourget N,Bouhnik Y,Bisetti N,Simonet JM,Decaris B

更新日期：1994-05-01 00:00:00