Neutral sugar composition and gravimetric yield of plant and bacterial fractions of feces.

Abstract:

:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blended rat and human feces. Three screen mech combinations (150 and 75 microns, 150 and 35 microns, 35 microns) were used with rat feces. Filtration of a homogenized rat fecal slurry sequentially through 150- and 35-microns-mesh screens versus 150- and 75-microns-mesh screens decreased the gravimetric recovery of bacteria from congruent to 35 to congruent to 25% of fecal dry weight and increased the plant fraction weight. Neutral sugar composition, determined by gas chromatography of alditol acetates, and bacterial counts of the fractions suggested that the decreased yield of bacterial fraction represented removal of plant material and not a loss of bacteria. Rat excreta contained 29.5% (dry weight) total neutral sugar, 88% of which was recovered in the plant material. Human feces containing wheat bran, fractionated with the 150- and 35-microns-mesh screens, was 21% neutral sugar, congruent to 65% of which was in the plant fraction. The plant fractions had more xylose and arabinose and less glucose than the bacterial fractions. Processing samples in a Waring blender had no adverse effect on the rat or human fecal bacterial counts. The use of this gravimetric method in combination with the sugar analysis of the fractions provided a better measure of plant and bacteria than only gravimetric yield.

journal_name

Appl Environ Microbiol

authors

Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

doi

10.1128/AEM.56.6.1786-1792.1990

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

1786-92

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

56

pub_type

杂志文章
  • Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale.

    abstract::Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00335-09

    authors: Lauber CL,Hamady M,Knight R,Fierer N

    更新日期:2009-08-01 00:00:00

  • Effects of fixation on cell volume of marine planktonic protozoa.

    abstract::The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.7.1761-1765.1989

    authors: Choi JW,Stoecker DK

    更新日期:1989-07-01 00:00:00

  • Agar overlay method to measure adherence of Staphylococcus epidermidis to four plastic surfaces.

    abstract::The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.5.1322-1324.1985

    authors: Mackenzie AM,Rivera-Calderon RL

    更新日期:1985-11-01 00:00:00

  • Clinically Unreported Salmonellosis Outbreak Detected via Comparative Genomic Analysis of Municipal Wastewater Salmonella Isolates.

    abstract::Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00139-19

    authors: Diemert S,Yan T

    更新日期:2019-05-02 00:00:00

  • Bacterial degradation of emulsan.

    abstract::Emulsan is a polyanionic heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1. A mixed bacterial population was obtained by enrichment culture that was capable of degrading emulsan and using it as a carbon source. From this mixed culture, an emulsan-degrading bacterium, termed YUV-1, was is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.3.573-579.1983

    authors: Shoham Y,Rosenberg M,Rosenberg E

    更新日期:1983-09-01 00:00:00

  • Defined medium for Moraxella (Branhamella) catarrhalis.

    abstract::A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glyci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.3.546-551.1986

    authors: Juni E,Heym GA,Avery M

    更新日期:1986-09-01 00:00:00

  • Quantitative determination of the biodegradable polymer Poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics.

    abstract::Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with ga...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3415-3420.2000

    authors: Kansiz M,Billman-Jacobe H,McNaughton D

    更新日期:2000-08-01 00:00:00

  • Isolation and characterization of Micromonospora phage PhiHAU8 and development into a phasmid.

    abstract::PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Dif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.3893-3897.2004

    authors: Li X,Zhou X,Deng Z

    更新日期:2004-07-01 00:00:00

  • Shifts in identity and activity of methanotrophs in arctic lake sediments in response to temperature changes.

    abstract::Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00853-12

    authors: He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

    更新日期:2012-07-01 00:00:00

  • Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry.

    abstract::The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01777-06

    authors: Dinoto A,Marques TM,Sakamoto K,Fukiya S,Watanabe J,Ito S,Yokota A

    更新日期:2006-12-01 00:00:00

  • Influence of transition metals added during sporulation on heat resistance of Clostridium botulinum 113B spores.

    abstract::Sporulation of Clostridium botulinum 113B in a complex medium supplemented with certain transition metals (Fe, Mn, Cu, or Zn) at 0.01 to 1.0 mM gave spores that were increased two to sevenfold in their contents of the added metals. The contents of calcium, magnesium, and other metals in the purified spores were relati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.3.681-685.1990

    authors: Kihm DJ,Hutton MT,Hanlin JH,Johnson EA

    更新日期:1990-03-01 00:00:00

  • Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed.

    abstract::A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6053-6060.2004

    authors: Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

    更新日期:2004-10-01 00:00:00

  • Plasmid profiles as indicators of the source of contamination of Staphylococcus aureus endemic within poultry processing plants.

    abstract::A total of 530 strains of Staphylococcus aureus were isolated from the defeathering machinery of a chicken processing plant and from neck skin samples of carcasses at different stages of processing in two visits 4 weeks apart. Eleven different plasmid profiles were detected in the isolates, eight being common to both ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1541-1549.1988

    authors: Dodd CE,Chaffey BJ,Waites WM

    更新日期:1988-06-01 00:00:00

  • Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia.

    abstract::Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3425-3433.2004

    authors: Hewson I,Fuhrman JA

    更新日期:2004-06-01 00:00:00

  • Identification of disulfides from the biodegradation of dibenzothiophene.

    abstract::Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5084-5093.2001

    authors: Bressler DC,Fedorak PM

    更新日期:2001-11-01 00:00:00

  • Viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in Finland.

    abstract::During an outbreak of paralytic poliomyelitis in Finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater Helsinki district and in 13 other cities o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.371-374.1988

    authors: Pöyry T,Stenvik M,Hovi T

    更新日期:1988-02-01 00:00:00

  • Microbiome Structure Influences Infection by the Parasite Crithidia bombi in Bumble Bees.

    abstract::Recent declines in bumble bee populations are of great concern and have prompted critical evaluations of the role of pathogen introductions and host resistance in bee health. One factor that may influence host resilience when facing infection is the gut microbiota. Previous experiments with Bombus terrestris, a Europe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02335-17

    authors: Mockler BK,Kwong WK,Moran NA,Koch H

    更新日期:2018-03-19 00:00:00

  • Carbon monoxide metabolism in roadside soils.

    abstract::Air-dried soils which were equilibrated under relative humidities greater than 93% or moistened with liquid water showed marked increases in their capacities to oxidize CO to CO(2). Liquid water addition in excess of saturation resulted in lower CO oxidation rates, reflecting the limited diffusion of CO through the aq...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.5.1192-1201.1981

    authors: Spratt HG,Hubbard JS

    更新日期:1981-05-01 00:00:00

  • Possible waterborne transmission and maintenance of influenza viruses in domestic ducks.

    abstract::Two duck farms in Hong Kong were examined monthly for 1 year for the occurrence and persistence of influenza viruses within the duck communities. The predominant virus in one community was H3N2, a virus antigenically related to the pandemic Hong Kong strain. This virus was isolated monthly throughout the year from fec...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.110-115.1982

    authors: Markwell DD,Shortridge KF

    更新日期:1982-01-01 00:00:00

  • Two Different Bacillus thuringiensis Delta-Endotoxin Receptors in the Midgut Brush Border Membrane of the European Corn Borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Pyralidae).

    abstract::Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.6.1828-1837.1993

    authors: Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

    更新日期:1993-06-01 00:00:00

  • Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.

    abstract::Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.4117-4120.2005

    authors: Fierer N,Jackson JA,Vilgalys R,Jackson RB

    更新日期:2005-07-01 00:00:00

  • Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita.

    abstract::In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5646-5649.2005

    authors: Siddiqui IA,Haas D,Heeb S

    更新日期:2005-09-01 00:00:00

  • Evaluation of the Efficacy of Methyl Bromide in the Decontamination of Building and Interior Materials Contaminated with Bacillus anthracis Spores.

    abstract::The primary goal of this study was to determine the conditions required for the effective inactivation of Bacillus anthracis spores on materials by using methyl bromide (MeBr) gas. Another objective was to obtain comparative decontamination efficacy data with three avirulent microorganisms to assess their potential fo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03445-15

    authors: Wood JP,Wendling M,Richter W,Lastivka A,Mickelsen L

    更新日期:2016-01-22 00:00:00

  • Spontaneous formation of a mannitol-producing variant of Leuconostoc pseudomesenteroides grown in the presence of fructose.

    abstract::We report the spontaneous formation of a stable mannitol-producing variant of Leuconostoc pseudomesenteroides. The mannitol-producing variant showed mannitol dehydrogenase activity which was absent in the parental strain. It was also able to use fructose and glucose simultaneously, whereas the parental strain showed d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2867-2870.2001

    authors: Grobben GJ,Peters SW,Wisselink HW,Weusthuis RA,Hoefnagel MH,Hugenholtz J,Eggink G

    更新日期:2001-06-01 00:00:00

  • Methylosinus trichosporium OB3b Mutants Having Constitutive Expression of Soluble Methane Monooxygenase in the Presence of High Levels of Copper.

    abstract::The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.11.3701-3708.1992

    authors: Phelps PA,Agarwal SK,Speitel GE,Georgiou G

    更新日期:1992-11-01 00:00:00

  • Conjugal transfer of R-plasmid R1drd-19 in Escherichia coli below 22 degrees C.

    abstract::The conjugal transfer of R-plasmids is known to occur at temperatures above 22 degrees C. We found that R1drd-19 is transferable below 22 degrees C, and we discuss this finding in the context of plasmid transfer in environmental waters. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.5.789-791.1981

    authors: Singleton P,Anson AE

    更新日期:1981-11-01 00:00:00

  • Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138.

    abstract::A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4454-4458.1995

    authors: Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

    更新日期:1995-12-01 00:00:00

  • Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius.

    abstract::Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.8.2453-2461.1990

    authors: Moody SF,Tyler BM

    更新日期:1990-08-01 00:00:00

  • Molecular indicators used in the development of predictive models for microbial source tracking.

    abstract::A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02350-09

    authors: Ballesté E,Bonjoch X,Belanche LA,Blanch AR

    更新日期:2010-03-01 00:00:00

  • A proposed pathway for catabolism of propionate in methanogenic cocultures.

    abstract::A metabolic pathway for the catabolism of propionate is proposed. This pathway incorporates a transcarboxylation reaction involving propionyl coenzyme A and oxaloacetate and a carboxylation of pyruvate to regenerate oxaloacetate. Data indicated that the proposed pathway is reversible. The proposed pathway and its appa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1300-1301.1988

    authors: Robbins JE

    更新日期:1988-05-01 00:00:00