Abstract:
:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blended rat and human feces. Three screen mech combinations (150 and 75 microns, 150 and 35 microns, 35 microns) were used with rat feces. Filtration of a homogenized rat fecal slurry sequentially through 150- and 35-microns-mesh screens versus 150- and 75-microns-mesh screens decreased the gravimetric recovery of bacteria from congruent to 35 to congruent to 25% of fecal dry weight and increased the plant fraction weight. Neutral sugar composition, determined by gas chromatography of alditol acetates, and bacterial counts of the fractions suggested that the decreased yield of bacterial fraction represented removal of plant material and not a loss of bacteria. Rat excreta contained 29.5% (dry weight) total neutral sugar, 88% of which was recovered in the plant material. Human feces containing wheat bran, fractionated with the 150- and 35-microns-mesh screens, was 21% neutral sugar, congruent to 65% of which was in the plant fraction. The plant fractions had more xylose and arabinose and less glucose than the bacterial fractions. Processing samples in a Waring blender had no adverse effect on the rat or human fecal bacterial counts. The use of this gravimetric method in combination with the sugar analysis of the fractions provided a better measure of plant and bacteria than only gravimetric yield.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JAdoi
10.1128/AEM.56.6.1786-1792.1990subject
Has Abstractpub_date
1990-06-01 00:00:00pages
1786-92issue
6eissn
0099-2240issn
1098-5336journal_volume
56pub_type
杂志文章abstract::Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...
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更新日期:2004-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.6.1541-1549.1988
更新日期:1988-06-01 00:00:00
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更新日期:2001-11-01 00:00:00
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doi:10.1128/AEM.54.2.371-374.1988
更新日期:1988-02-01 00:00:00
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更新日期:1981-05-01 00:00:00
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更新日期:1981-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.8.2453-2461.1990
更新日期:1990-08-01 00:00:00
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doi:10.1128/AEM.54.5.1300-1301.1988
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