Xylanase IV, an Exoxylanase of Aeromonas caviae ME-1 Which Produces Xylotetraose as the Only Low-Molecular-Weight Oligosaccharide from Xylan.

abstract：:We compared the influences of grazing by the bacterivorous nanoflagellate Poterioochromonas sp. strain DS on ultramicrobacterial Actinobacteria affiliated with the Luna-2 cluster and ultramicrobacterial Betaproteobacteria of the species Polynucleobacter cosmopolitanus. These bacteria were almost identical in size (<0....

journal_title：Applied and environmental microbiology

authors： Tarao M,Jezbera J,Hahn MW

更新日期：2009-07-01 00:00:00

abstract：:Prophages make up 12% of the enterohemorrhagic Escherichia coli genome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among ...

journal_title：Applied and environmental microbiology

authors： Chen C,Lewis CR,Goswami K,Roberts EL,DebRoy C,Dudley EG

更新日期：2013-03-01 00:00:00

abstract：:Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, intro...

journal_title：Applied and environmental microbiology

authors： Mizutani O,Masaki K,Gomi K,Iefuji H

更新日期：2012-06-01 00:00:00

abstract：:The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW expo...

journal_title：Applied and environmental microbiology

authors： Rougier C,Prorot A,Chazal P,Leveque P,Leprat P

更新日期：2014-08-01 00:00:00

abstract：:Inorganic phosphorus (Pi) is one of the main growth-limiting factors of diazotrophic cyanobacteria. Due to human activity, the availability of Pi has increased in water bodies, resulting in eutrophication and the formation of massive cyanobacterial blooms. In this study, we examined the molecular responses of the cyan...

journal_title：Applied and environmental microbiology

authors： Teikari J,Österholm J,Kopf M,Battchikova N,Wahlsten M,Aro EM,Hess WR,Sivonen K

更新日期：2015-08-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:Progesterone was converted to 5alpha-pregnane-3alpha-ol-20-one, delta4-pregnene-20alpha-ol-3-one, delta4-pregnene-14alpha-ol-3,20-dione, delta4-pregnene-7beta,14alpha-diol-3,20-dione, and delta4-pregnene-6beta,11alpha-diol-3,20-dione by cell cultures of Lycopersicon esculentum. Cell cultures of Capsicum frutescens (gr...

journal_title：Applied and environmental microbiology

authors： Yagen B,Gallili GE,Mateles RI

更新日期：1978-08-01 00:00:00

abstract：:The influence of trichloroethene (TCE; 0 to 65 mg/liter) and 1,1,1-trichloroethane (1,1,1-TCA; 0 to 103 mg/liter) on methane consumption of a mixed culture of methane-oxidizing bacteria was studied in laboratory batch experiments. Increasing concentrations of TCE or 1,1,1-TCA resulted in decreasing methane consumption...

journal_title：Applied and environmental microbiology

authors： Broholm K,Jensen BK,Christensen TH,Olsen L

更新日期：1990-08-01 00:00:00

abstract：:The exopolysaccharides (EPSs) produced by some bacteria are potential growth substrates for other bacteria in soil. We used stable-isotope probing (SIP) to identify aerobic soil bacteria that assimilated the cellulose produced by Gluconacetobacter xylinus or the EPS produced by Beijerinckia indica. The latter is a het...

journal_title：Applied and environmental microbiology

authors： Wang X,Sharp CE,Jones GM,Grasby SE,Brady AL,Dunfield PF

更新日期：2015-07-01 00:00:00

abstract：UNLABELLED:We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrat...

journal_title：Applied and environmental microbiology

authors： Wilding M,Peat TS,Newman J,Scott C

更新日期：2016-06-13 00:00:00

abstract：:A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or s...

journal_title：Applied and environmental microbiology

authors： Wegmüller B,Lüthy J,Candrian U

更新日期：1993-07-01 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:An efficient method for the transformation of the methylotrophic bacterium Methylobacterium extorquens NR-2 with a broad-host-range plasmid, pLA2917, by electroporation was examined. Transformants of M. extorquens NR-2 expressing resistance to kanamycin were obtained after electric pulse. These transformants were foun...

journal_title：Applied and environmental microbiology

authors： Ueda S,Matsumoto S,Shimizu S,Yamane T

更新日期：1991-04-01 00:00:00

abstract：:Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

journal_title：Applied and environmental microbiology

authors： Morales SE,Holben WE

更新日期：2009-05-01 00:00:00

abstract：:Organically raised swine had high fecal populations of chlortetracycline (CTC)-resistant (growing at 64 μg CTC/ml) Escherichia coli, Megasphaera elsdenii, and anaerobic bacteria. By comparison, CTC-resistant bacteria in feral swine feces were over 1,000-fold fewer and exhibited lower taxonomic diversity. ...

journal_title：Applied and environmental microbiology

authors： Stanton TB,Humphrey SB,Stoffregen WC

更新日期：2011-10-01 00:00:00

abstract：:A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

journal_title：Applied and environmental microbiology

authors： Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

更新日期：1997-03-01 00:00:00

abstract：:Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific ac...

journal_title：Applied and environmental microbiology

authors： Han J,Lu Q,Zhou L,Liu H,Xiang H

更新日期：2009-10-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:Norwalk virus and other human caliciviruses (noroviruses) are major agents of gastroenteritis, and water is a major route of their transmission. In an effort to control Norwalk virus in drinking water, Norwalk virus reduction by bench-scale ozone disinfection was determined using quantitative reverse transcription (RT...

journal_title：Applied and environmental microbiology

authors： Shin GA,Sobsey MD

更新日期：2003-07-01 00:00:00

abstract：:The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

journal_title：Applied and environmental microbiology

authors： Yu EK,Saddler JN

更新日期：1983-09-01 00:00:00

abstract：:Cyanobacterial blooms are potential health hazards in water supply reservoirs. This paper reports analyses of a cyanobacterial bloom by use of PCR-based methods for direct detection and identification of strains present and determination of their toxigenicity. Serial samples from Malpas Dam, in the New England region ...

journal_title：Applied and environmental microbiology

authors： Baker JA,Entsch B,Neilan BA,McKay DB

更新日期：2002-12-01 00:00:00

abstract：:Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

journal_title：Applied and environmental microbiology

authors： Halpern M,Gancz H,Broza M,Kashi Y

更新日期：2003-07-01 00:00:00

abstract：:The gray mold fungus Botrytis cinerea is a major threat to fruit and vegetable production. Strawberry fields usually receive several fungicide treatments against Botrytis per season. Gray mold isolates from several German strawberry-growing regions were analyzed to determine their sensitivity against botryticides. Fun...

journal_title：Applied and environmental microbiology

authors： Leroch M,Plesken C,Weber RW,Kauff F,Scalliet G,Hahn M

更新日期：2013-01-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis that grows to a higher cell density (SE-HCD) than wild type while retaining O-chain lipopolysaccharide was isolated by transforming wild type serovar Enteritidis with the cell density sensor plasmid pSB402 and selecting for bioluminescence. A luminescent strain, SE-HCD, that emi...

journal_title：Applied and environmental microbiology

authors： Guard-Petter J

更新日期：1998-06-01 00:00:00

abstract：:A thermophilic strain (D2) identified as a Bacillus sp. was isolated from an aerobic digestor of swine waste after several months of operation at 55 degrees C. Aerobic thermophilic batch treatment of swine waste inoculated with strain D2 was studied in a 4-liter fixed-bed reactor. Stabilization of the waste was achiev...

journal_title：Applied and environmental microbiology

authors： Beaudet R,Gagnon C,Bisaillon JG,Ishaque M

更新日期：1990-04-01 00:00:00

abstract：:A small spirillum, designated 5175, was isolated from an anaerobic enrichment culture for Desulfuromonas in which the major medium constituents were acetate and elemental sulfur. The organisms grew only under anaerobic or microaerophilic conditions. Elemental sulfur was formed anaerobically in a malate-sulfide medium,...

journal_title：Applied and environmental microbiology

authors： Wolfe RS,Penning N

更新日期：1977-02-01 00:00:00

abstract：:Cytochrome P450 BM-3, a self-sufficient P450 enzyme from Bacillus megaterium that catalyzes the subterminal hydroxylation of long-chain fatty acids, has been engineered into a catalyst for the oxidation of polycyclic aromatic hydrocarbons. The activities of a triplet mutant (A74G/F87V/L188Q) towards naphthalene, fluor...

journal_title：Applied and environmental microbiology

authors： Li QS,Ogawa J,Schmid RD,Shimizu S

更新日期：2001-12-01 00:00:00

abstract：:1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli express...

journal_title：Applied and environmental microbiology

authors： Altaras NE,Cameron DC

更新日期：1999-03-01 00:00:00

abstract：:We investigated the anaerobic biodegradation of 3-chlorobenzoate (3CBz) by microorganisms from an aquifer where chloroaromatic compounds were previously found to resist decay in the presence of sulfate. After a lengthy lag period, 3CBz was degraded in the presence of sulfate and concurrently with sulfate reduction. Ch...

journal_title：Applied and environmental microbiology

authors： Townsend GT,Ramanand K,Suflita JM

更新日期：1997-07-01 00:00:00