Selection of clc, cba, and fcb chlorobenzoate-catabolic genotypes from groundwater and surface waters adjacent to the Hyde park, Niagara Falls, chemical landfill.

abstract：:We used a combination of stable isotope probing (SIP), gas chromatography-mass spectrometry-based respiration, isolation/cultivation, and quantitative PCR procedures to discover the identity and in situ growth of soil microorganisms that metabolize benzoic acid. We added [(13)C]benzoic acid or [(12)C]benzoic acid (100...

journal_title：Applied and environmental microbiology

authors： Pumphrey GM,Madsen EL

更新日期：2008-07-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Microscopic eukaryotes are known to have important ecosystem functions, but their diversity in most environments remains vastly unexplored. Here we analyzed an 18S rRNA gene library from a subsurface iron- and sulfur-oxidizing microbial community growing in highly acidic (pH < 0.9) runoff within the Richmond Mine at I...

journal_title：Applied and environmental microbiology

authors： Baker BJ,Tyson GW,Goosherst L,Banfield JF

更新日期：2009-04-01 00:00:00

abstract：:Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

更新日期：2015-12-18 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00

abstract：:A metabolic pathway for the catabolism of propionate is proposed. This pathway incorporates a transcarboxylation reaction involving propionyl coenzyme A and oxaloacetate and a carboxylation of pyruvate to regenerate oxaloacetate. Data indicated that the proposed pathway is reversible. The proposed pathway and its appa...

journal_title：Applied and environmental microbiology

authors： Robbins JE

更新日期：1988-05-01 00:00:00

abstract：:Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-alpha2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-density cultivat...

journal_title：Applied and environmental microbiology

authors： Sletta H,Tøndervik A,Hakvåg S,Aune TE,Nedal A,Aune R,Evensen G,Valla S,Ellingsen TE,Brautaset T

更新日期：2007-02-01 00:00:00

abstract：:Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

journal_title：Applied and environmental microbiology

authors： Ji P,Wilson M

更新日期：2003-02-01 00:00:00

abstract：:Bacillus thuringiensis serovar israelensis (B. thuringiensis subsp. israelensis) produces four insecticidal crystal proteins (ICPs) (Cry4A, Cry4B, Cry11A, and Cyt1A). Toxicity of recombinant B. thuringiensis subsp. israelensis strains expressing only one of the toxins was determined with first instars of Tipula paludo...

journal_title：Applied and environmental microbiology

authors： Oestergaard J,Ehlers RU,Martínez-Ramírez AC,Real MD

更新日期：2007-06-01 00:00:00

abstract：:Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

journal_title：Applied and environmental microbiology

authors： Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

更新日期：2007-05-01 00:00:00

abstract：:Bacterial signaling is an important part of community life, but little is known about the signal transduction pathways of the as-yet-uncultured members of microbial communities. To address this gap, we aimed to identify genes directing the synthesis of signals in uncultured bacteria associated with the midguts of gyps...

journal_title：Applied and environmental microbiology

authors： Guan C,Ju J,Borlee BR,Williamson LL,Shen B,Raffa KF,Handelsman J

更新日期：2007-06-01 00:00:00

abstract：:Broad-substrate-range monooygenase enzymes, including toluene-4-monooxygenase (T4MO), can catalyze the oxidation of indole. The indole oxidation products can then condense to form the industrially important dye indigo. Site-directed mutagenesis of T4MO resulted in the creation of T4MO isoforms with altered pigment pro...

journal_title：Applied and environmental microbiology

authors： McClay K,Boss C,Keresztes I,Steffan RJ

更新日期：2005-09-01 00:00:00

abstract：:Estuarine systems are the major conduits for the transfer of nitrate from agricultural and other terrestrial-anthropogenic sources into marine ecosystems. Within estuarine sediments some microbially driven processes (denitrification and anammox) result in the net removal of nitrogen from the environment, while others ...

journal_title：Applied and environmental microbiology

authors： Smith CJ,Nedwell DB,Dong LF,Osborn AM

更新日期：2007-06-01 00:00:00

abstract：:Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most clo...

journal_title：Applied and environmental microbiology

authors： Nunan N,Daniell TJ,Singh BK,Papert A,McNicol JW,Prosser JI

更新日期：2005-11-01 00:00:00

abstract：:Phytophthora ramorum is a recently described pathogen causing oak mortality (sudden oak death) in forests in coastal areas of California and southern Oregon and dieback and leaf blight in a range of tree, shrub, and herbaceous species in the United States and Europe. Due to the threat posed by this organism, stringent...

journal_title：Applied and environmental microbiology

authors： Tomlinson JA,Boonham N,Hughes KJ,Griffin RL,Barker I

更新日期：2005-11-01 00:00:00

abstract：:Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

journal_title：Applied and environmental microbiology

authors： Miller LK,Dawes KP

更新日期：1978-06-01 00:00:00

abstract：:Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...

journal_title：Applied and environmental microbiology

authors： Klecka GM,Gibson DT

更新日期：1980-02-01 00:00:00

abstract：:A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp ...

journal_title：Applied and environmental microbiology

authors： Sahl JW,Schmidt R,Swanner ED,Mandernack KW,Templeton AS,Kieft TL,Smith RL,Sanford WE,Callaghan RL,Mitton JB,Spear JR

更新日期：2008-01-01 00:00:00

abstract：:Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional m...

journal_title：Applied and environmental microbiology

authors： Hornstra LM,de Vries YP,de Vos WM,Abee T,Wells-Bennik MH

更新日期：2005-02-01 00:00:00

abstract：:Escherichia coli JM101(pSPZ3), containing xylene monooxygenase (XMO) from Pseudomonas putida mt-2, catalyzes specific oxidations and reductions of m-nitrotoluene and derivatives thereof. In addition to reactions catalyzed by XMO, we focused on biotransformations by native enzymes of the E. coli host and their effect o...

journal_title：Applied and environmental microbiology

authors： Meyer D,Witholt B,Schmid A

更新日期：2005-11-01 00:00:00

abstract：:The growth response of Acacia mangium Willd. to inoculation with selected Bradyrhizobium strains was investigated in two field trials in the Ivory Coast (West Africa). In the first trial (Anguededou), four provenances (i.e., trees originating from seeds harvested in different geographical areas) of A. mangium were ino...

journal_title：Applied and environmental microbiology

authors： Galiana A,Prin Y,Mallet B,Gnahoua GM,Poitel M,Diem HG

更新日期：1994-11-01 00:00:00

abstract：:The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded i...

journal_title：Applied and environmental microbiology

authors： Kane SR,Beller HR,Legler TC,Koester CJ,Pinkart HC,Halden RU,Happel AM

更新日期：2001-12-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:Commercial Atlantic blue crabs (Callinectes sapidus) were exposed to 2.0x10(4) infectious waterborne oocysts of Cryptosporidium parvum. The study demonstrated that blue crabs can transfer C. parvum oocysts to persons involved in handling or preparing crabs and that they may contaminate other surfaces or products durin...

journal_title：Applied and environmental microbiology

authors： Graczyk TK,McOliver C,Silbergeld EK,Tamang L,Roberts JD

更新日期：2007-06-01 00:00:00

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence...

journal_title：Applied and environmental microbiology

authors： Pichard SL,Paul JH

更新日期：1993-02-01 00:00:00

abstract：:Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

journal_title：Applied and environmental microbiology

authors： Li FM,Hu HY

更新日期：2005-11-01 00:00:00

abstract：:3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...

journal_title：Applied and environmental microbiology

authors： Zhang JY,Zou J,Bao Q,Chen WL,Wang L,Yang H,Zhang CC

更新日期：2006-01-01 00:00:00