Abstract:
:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as long as ammonium chloride was omitted. Ammonium chloride caused either a lag or a reduction or a complete inhibition of the growth of A. serpens VHL on the above amino acids and other organic supplements depending on the combination used. Ammonium sulfate and ammonium hydroxide with L-glutamic acid allowed growth, but the lag period was increased in shake flask cultures. Vitamins, cysteine hydrochloride, and carbon dioxide had no effect on the growth rate. Viability (less than 50%) was inadequate to maintain continuous culture with L-glutamic acid as the sole source of carbon and nitrogen. Combinations of amino and carboxylic acids were then tested and, of these, L-glutamic acid (1 g/liter) and L-histidine (75 mg/liter) without ammonium chloride in the basal salts medium supported growth in batch and continuous culture. L-Glutamic acid was the limiting substrate for growth.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Whitby GE,Murray RGdoi
10.1128/AEM.39.1.20-24.1980subject
Has Abstractpub_date
1980-01-01 00:00:00pages
20-4issue
1eissn
0099-2240issn
1098-5336journal_volume
39pub_type
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doi:10.1128/AEM.43.2.447-453.1982
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doi:10.1128/AEM.60.9.3112-3119.1994
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journal_title:Applied and environmental microbiology
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更新日期:1992-05-01 00:00:00
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更新日期:1988-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00