Bacterial fermentation of cheese whey for production of a ruminant feed supplement rich in curde protein.

abstract：:The blacklegged tick, Ixodes scapularis, is of significant public health importance as a vector of Borrelia burgdorferi, the agent of Lyme borreliosis. The timing of seasonal activity of each immature I. scapularis life stage relative to the next is critical for the maintenance of B. burgdorferi because larvae must fe...

journal_title：Applied and environmental microbiology

authors： Gatewood AG,Liebman KA,Vourc'h G,Bunikis J,Hamer SA,Cortinas R,Melton F,Cislo P,Kitron U,Tsao J,Barbour AG,Fish D,Diuk-Wasser MA

更新日期：2009-04-01 00:00:00

abstract：:The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

journal_title：Applied and environmental microbiology

authors： Abe J,Ushijima C,Hizukuri S

更新日期：1999-09-01 00:00:00

abstract：:In this study, the changes in the global proteome of Salmonella in response to desiccation and thermal treatment were investigated by using an iTRAQ multiplex technique. A Salmonella enterica serovar Typhimurium strain was dried, equilibrated at high (1.0) and low (0.11) water activity (aw), and thermally treated at 7...

journal_title：Applied and environmental microbiology

authors： Maserati A,Lourenco A,Diez-Gonzalez F,Fink RC

更新日期：2018-08-31 00:00:00

abstract：:Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

更新日期：2005-08-01 00:00:00

abstract：:In Agrobacterium tumefaciens, the balance between acquiring enough iron and avoiding iron-induced toxicity is regulated in part by Fur (ferric uptake regulator). A fur mutant was constructed to address the physiological role of the regulator. Atypically, the mutant did not show alterations in the levels of siderophore...

journal_title：Applied and environmental microbiology

authors： Kitphati W,Ngok-Ngam P,Suwanmaneerat S,Sukchawalit R,Mongkolsuk S

更新日期：2007-08-01 00:00:00

abstract：:Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are l-rhamnose and d-glucose. C. beijerinckii DSM 6423 ut...

journal_title：Applied and environmental microbiology

authors： Diallo M,Simons AD,van der Wal H,Collas F,Houweling-Tan B,Kengen SWM,López-Contreras AM

更新日期：2019-02-20 00:00:00

abstract：:Recombinant Escherichia coli fadR atoC(Con) mutants containing the polyhydroxyalkanoate (PHA) biosynthesis genes from Alcaligenes eutrophus are able to incorporate significant levels of 3-hydroxyvalerate (3HV) into the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]. We have used E. coli fadR (F...

journal_title：Applied and environmental microbiology

authors： Rhie HG,Dennis D

更新日期：1995-07-01 00:00:00

abstract：:Nitrogen and oxygen transformations were studied in a bioturbated (reworked by animals) estuarine sediment (Norsminde Fjord, Denmark) by using a combination of N isotope (NO(3)), specific inhibitor (C(2)H(2)), and microsensor (N(2)O and O(2)) techniques in a continuous-flow core system. The estuarine water was NO(3) r...

journal_title：Applied and environmental microbiology

authors： Binnerup SJ,Jensen K,Revsbech NP,Jensen MH,Sørensen J

更新日期：1992-01-01 00:00:00

abstract：:A total of 286 isolates of Pseudomonas maltophilia was collected from sheep exhibiting brown or yellowish fleece rot and from fly-strike lesions. Enzyme activities for 10 of the isolates were examined by plate tests and with the API ZYM system and compared with the enzymatic profile of a human type strain of P. maltop...

journal_title：Applied and environmental microbiology

authors： Macdiarmid JA,Burrell DH

更新日期：1986-02-01 00:00:00

abstract：:beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

journal_title：Applied and environmental microbiology

authors： Rao MV,Dutta SM

更新日期：1977-08-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...

journal_title：Applied and environmental microbiology

authors： Robinson IM,Whipp SC,Bucklin JA,Allison MJ

更新日期：1984-11-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:Two different Cd(2+) uptake systems were identified in Lactobacillus plantarum. One is a high-affinity, high-velocity Mn(2+) uptake system which also takes up Cd(2+) and is induced by Mn(2+) starvation. The calculated K(m) and V(max) are 0.26 microM and 3.6 micromol g of dry cell(-1) min(-1), respectively. Unlike Mn(2...

journal_title：Applied and environmental microbiology

authors： Hao Z,Reiske HR,Wilson DB

更新日期：1999-11-01 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not ind...

journal_title：Applied and environmental microbiology

authors： Abbaszadegan M,Huber MS,Gerba CP,Pepper IL

更新日期：1997-01-01 00:00:00

abstract：:Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 x 10(4) and 4.5 ...

journal_title：Applied and environmental microbiology

authors： Lumb R,Stapledon R,Scroop A,Bond P,Cunliffe D,Goodwin A,Doyle R,Bastian I

更新日期：2004-08-01 00:00:00

abstract：:SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. ...

journal_title：Applied and environmental microbiology

authors： Benbadis L,Garel JR,Hartley DL

更新日期：1991-12-01 00:00:00

abstract：:The thermophilic homoacetogenic bacterium Moorella sp. strain HUC22-1 ferments glyoxylate to acetate roughly according to the reaction 2 glyoxylate --> acetate + 2 CO(2). A batch culture with glyoxylate and yeast extract yielded 11.7 g per mol of cells per substrate, which was much higher than that obtained with H(2) ...

journal_title：Applied and environmental microbiology

authors： Sakai S,Inokuma K,Nakashimada Y,Nishio N

更新日期：2008-03-01 00:00:00

abstract：:Five commercially available plastic containers were compared with glass for toxicity toward Babesia rodhaini and Babesia bigemina. Comparisons were made by using infectivity tests in mice (B. rodhaini) and cattle (B. bigemina). Low-density polypropylene and polystyrene containers were not toxic, but two of the three p...

journal_title：Applied and environmental microbiology

authors： Timms P

更新日期：1981-01-01 00:00:00

abstract：:The effect of salinity and growth temperature on the accumulation of intracellular organic solutes was examined in the hyperthermophilic archaeon Palaeococcus ferrophilus. The genus Palaeococcus represents a deep-branching lineage of the order Thermococcales, which diverged before Thermococcus and Pyrococcus. Palaeoco...

journal_title：Applied and environmental microbiology

authors： Neves C,da Costa MS,Santos H

更新日期：2005-12-01 00:00:00

abstract：:A method was developed to deposit Bacillus subtilis spores via aerosolization onto various surface materials for biological agent decontamination and detection studies. This new method uses an apparatus coupled with a metered dose inhaler to reproducibly deposit spores onto various surfaces. A metered dose inhaler was...

journal_title：Applied and environmental microbiology

authors： Lee SD,Ryan SP,Snyder EG

更新日期：2011-03-01 00:00:00

abstract：:Arthrobacter viscosus NRRL B-1973 produces its viscous extracellular polysaccharides (EPS) when grown on media containing xylose or enzymatic xylan hydrolysates. Crude EPS formation from xylose averaged 12 g/liter when initial culture pH was adjusted to 8.0 and total nitrogen was limited to 0.03%. Purified EPS from pe...

journal_title：Applied and environmental microbiology

authors： Novak JS,Tanenbaum SW,Nakas JP

更新日期：1992-11-01 00:00:00

abstract：:Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization ...

journal_title：Applied and environmental microbiology

authors： Konkel ME,Christensen JE,Dhillon AS,Lane AB,Hare-Sanford R,Schaberg DM,Larson CL

更新日期：2007-04-01 00:00:00

abstract：:The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...

journal_title：Applied and environmental microbiology

authors： Bebien M,Chauvin JP,Adriano JM,Grosse S,Verméglio A

更新日期：2001-10-01 00:00:00

abstract：:The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

journal_title：Applied and environmental microbiology

authors： Zappe H,Jones WA,Jones DT,Woods DR

更新日期：1988-05-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

journal_title：Applied and environmental microbiology

authors： Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

更新日期：2007-02-01 00:00:00