Abstract:
:Homologous recombination plays an important role in the structuring of genetic variation of many bacteria; however, its importance in adaptive evolution is not well established. We investigated the association of intersubspecific homologous recombination (IHR) with the shift to a novel host (mulberry) by the plant-pathogenic bacterium Xylella fastidiosa. Mulberry leaf scorch was identified about 25 years ago in native red mulberry in the eastern United States and has spread to introduced white mulberry in California. Comparing a sequence of 8 genes (4,706 bp) from 21 mulberry-type isolates to published data (352 isolates representing all subspecies), we confirmed previous indications that the mulberry isolates define a group distinct from the 4 subspecies, and we propose naming the taxon X. fastidiosa subsp. morus. The ancestry of its gene sequences was mixed, with 4 derived from X. fastidiosa subsp. fastidiosa (introduced from Central America), 3 from X. fastidiosa subsp. multiplex (considered native to the United States), and 1 chimeric, demonstrating that this group originated by large-scale IHR. The very low within-type genetic variation (0.08% site polymorphism), plus the apparent inability of native X. fastidiosa subsp. multiplex to infect mulberry, suggests that this host shift was achieved after strong selection acted on genetic variants created by IHR. Sequence data indicate that a single ancestral IHR event gave rise not only to X. fastidiosa subsp. morus but also to the X. fastidiosa subsp. multiplex recombinant group which infects several hosts but is the only type naturally infecting blueberry, thus implicating this IHR in the invasion of at least two novel native hosts, mulberry and blueberry.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Nunney L,Schuenzel EL,Scally M,Bromley RE,Stouthamer Rdoi
10.1128/AEM.04112-13subject
Has Abstractpub_date
2014-05-01 00:00:00pages
3025-33issue
10eissn
0099-2240issn
1098-5336pii
AEM.04112-13journal_volume
80pub_type
杂志文章abstract::Two strains of mesophilic lactic acid bacteria, Streptococcus cremoris AM2 and Leuconostoc lactis CNRZ 1091, were grown in pure and mixed cultures in the presence or absence of citrate (15 mM) and at controlled (pH 6.5) or uncontrolled pH. Microbial cell densities at the end of growth, maximum growth rates, the pH dec...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.10.2527-2531.1988
更新日期:1988-10-01 00:00:00
abstract::The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01777-06
更新日期:2006-12-01 00:00:00
abstract::The gut microbiota is a diverse and dynamic ecological community that is increasingly recognized to play important roles in host metabolic, immunological, and behavioral functioning. As such, identifying new routes for manipulating the microbiota may provide valuable additional methods for improving host health. Dieta...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
doi:10.1128/AEM.02246-18
更新日期:2019-05-02 00:00:00
abstract::The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00520-15
更新日期:2015-06-15 00:00:00
abstract::Yersinia spp. were isolated from 34 of 500 birds representing nine species. The highest isolation rate, 5 of 21 (23.8%), was found in blue magpies (Cyanopia cyanus), followed by pheasants (Phasianus colchicus tohkaidi), 5 of 33 (15.2%); gray starlings (Sturnus cineraceus), 6 of 57 (10.5%); tree sparrows (Passer montan...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.1.198-200.1985
更新日期:1985-01-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Se...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.1.459-461.2001
更新日期:2001-01-01 00:00:00
abstract::Arhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the cla...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01509-14
更新日期:2014-11-01 00:00:00
abstract::Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.2.1226-1230.2004
更新日期:2004-02-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) causes serious foodborne disease worldwide. It produces the very potent Shiga toxin 2 (Stx2). The Stx2-encoding genes are located on a prophage, and production of the toxin is linked to the synthesis of Stx phages. There is, currently, no good treatment for EHEC infections, as...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00583-20
更新日期:2020-11-24 00:00:00
abstract:UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01669-16
更新日期:2016-09-16 00:00:00
abstract::Bacterial signaling is an important part of community life, but little is known about the signal transduction pathways of the as-yet-uncultured members of microbial communities. To address this gap, we aimed to identify genes directing the synthesis of signals in uncultured bacteria associated with the midguts of gyps...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02617-06
更新日期:2007-06-01 00:00:00
abstract::Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.9.3810-3819.1999
更新日期:1999-09-01 00:00:00
abstract::Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.32.6.777-780.1976
更新日期:1976-12-01 00:00:00
abstract::Volumn 62, no. 11, p. 4174, Abstract, lines 12 and 13: "para dechlorination was restricted from 18 to 34(deg)C" should read "para dechlorination was restricted to temperatures from 18 to 34(deg)C." [This corrects the article on p. 4174 in vol. 62.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.7.4117-4120.2005
更新日期:2005-07-01 00:00:00
abstract::It has been shown that the coexistence of methanogenesis and reductive acetogenesis in the hindgut of the wood-feeding termite Reticulitermes flavipes is based largely on the radial distribution of the respective microbial populations and relatively high hydrogen partial pressures in the gut lumen. Using Clark-type mi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.10.4490-4496.1999
更新日期:1999-10-01 00:00:00
abstract::beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.6.1954-1956.1990
更新日期:1990-06-01 00:00:00
abstract::The discovery of a letter intentionally filled with dried Bacillus anthracis spores in the office of a United States senator prompted the collection and quarantine of all mail in congressional buildings. This mail was subsequently searched for additional intentionally contaminated letters. A microbiological sampling s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00940-06
更新日期:2006-08-01 00:00:00
abstract::To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01135-14
更新日期:2014-09-01 00:00:00
abstract::The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.5.1322-1324.1985
更新日期:1985-11-01 00:00:00
abstract::Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03414-13
更新日期:2014-05-01 00:00:00
abstract::Bacterioplankton are fundamental components of marine ecosystems and influence the entire biosphere by contributing to the global biogeochemical cycles of key elements. Yet, there is a significant gap in knowledge about their diversity and specific activities, as well as environmental factors that shape their communit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00494-17
更新日期:2017-08-17 00:00:00
abstract::Cadmium is an important heavy metal pollutant. For this study, we investigated the effects of cadmium exposure on the oxidative stress responses of Xanthomonas campestris, a soil and plant pathogenic bacterium. The exposure of X. campestris to low concentrations of cadmium induces cross-protection against subsequent k...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.1843-1849.2005
更新日期:2005-04-01 00:00:00
abstract::The incidence of human infection with Campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. Although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. Environmental sources are believed...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.7.3115-3121.2001
更新日期:2001-07-01 00:00:00
abstract::Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.6.1299-1304.1986
更新日期:1986-12-01 00:00:00
abstract::Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detectable amounts of CH...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.11.3450-3457.1990
更新日期:1990-11-01 00:00:00
abstract::A glycosyltransferase, involved in the synthesis of cyclic maltosylmaltose [CMM; cyclo-{-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->}] from starch, was purified to homogeneity from the culture supernatant of Arthrobacter globiformis M6. The CMM-forming enzyme had a molecular mass...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1065-1071.2006
更新日期:2006-02-01 00:00:00
abstract::Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02203-13
更新日期:2014-01-01 00:00:00
abstract::Escherichia coli is a widely utilized indicator of the sanitary quality of bivalve molluscan shellfish sold for human consumption. However, it is now well documented that shellfish that meet the E. coli standards for human consumption may contain human enteric viruses that cause gastroenteritis and hepatitis. In this ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.4.1280-1285.2000
更新日期:2000-04-01 00:00:00
abstract::Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.11.6954-6962.2005
更新日期:2005-11-01 00:00:00