Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence.

abstract：:Five commercially available plastic containers were compared with glass for toxicity toward Babesia rodhaini and Babesia bigemina. Comparisons were made by using infectivity tests in mice (B. rodhaini) and cattle (B. bigemina). Low-density polypropylene and polystyrene containers were not toxic, but two of the three p...

journal_title：Applied and environmental microbiology

authors： Timms P

更新日期：1981-01-01 00:00:00

abstract：:The release of transgenic plants and microorganisms expressing truncated genes from various subspecies of Bacillus thuringiensis that encode active insecticidal toxins rather than inactive protoxins could result in the accumulation of these active proteins in soil, especially when bound on clays and other soil particl...

journal_title：Applied and environmental microbiology

authors： Tapp H,Stotzky G

更新日期：1995-05-01 00:00:00

abstract：:Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

journal_title：Applied and environmental microbiology

authors： Desjardins AE,Plattner RD,Vanmiddlesworth F

更新日期：1986-03-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00

abstract：:Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...

journal_title：Applied and environmental microbiology

authors： Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WR

更新日期：1984-05-01 00:00:00

abstract：:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...

journal_title：Applied and environmental microbiology

authors： Chow MS,Rouf MA

更新日期：1983-05-01 00:00:00

abstract：:Survival studies were conducted on Legionella pneumophila cells that had been grown intracellularly in Acanthamoeba polyphaga and then exposed to polyhexamethylene biguanide (PHMB), benzisothiazolone (BIT), and 5-chloro-N-methylisothiazolone (CMIT). Susceptibilities were also determined for L. pneumophila grown under ...

journal_title：Applied and environmental microbiology

authors： Barker J,Brown MR,Collier PJ,Farrell I,Gilbert P

更新日期：1992-08-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...

journal_title：Applied and environmental microbiology

authors： Gabler M,Fischer L

更新日期：1999-08-01 00:00:00

abstract：:A genomic clone bank of Acetobacter polyoxogenes NBI1028 constructed in Escherichia coli by use of the expression vector pUC18 was screened with antibody raised against membrane-bound aldehyde dehydrogenase (ALDH; 75 kilodaltons [kDa]) from A. polyoxogenes NBI1028. A clone that synthesized a 41-kDa protein cross-react...

journal_title：Applied and environmental microbiology

authors： Fukaya M,Tayama K,Tamaki T,Tagami H,Okumura H,Kawamura Y,Beppu T

更新日期：1989-01-01 00:00:00

abstract：:Temperature and relative humidity are major factors determining virus inactivation in the environment. This article reviews inactivation data regarding coronaviruses on surfaces and in liquids from published studies and develops secondary models to predict coronaviruses inactivation as a function of temperature and re...

journal_title：Applied and environmental microbiology

authors： Guillier L,Martin-Latil S,Chaix E,Thébault A,Pavio N,Le Poder S,Batéjat C,Biot F,Koch L,Schaffner DW,Sanaa M,Covid-19 Emergency Collective Expert Appraisal Group.

更新日期：2020-09-01 00:00:00

abstract：:Streptomyces subtilisin inhibitor-like proteins were found to be distributed widely in streptomycetes by using the combination of the convenient, newly developed plate assay system and an established liquid culture assay. Almost all the strains formerly categorized as Streptoverticillium species produced proteins that...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Kikuchi H,Suzuki M,Kojima S,Terabe M,Miura K,Nakase T,Momose H

更新日期：1993-12-01 00:00:00

abstract：:Antibody production against aflatoxin B1 was compared in three rabbits and one goat. Titers obtained were 20 times higher in the rabbits than in the goat. The goat antiserum appeared to have a higher degree of cross-reactivity for other aflatoxins and related metabolites than did the rabbit antiserum. ...

journal_title：Applied and environmental microbiology

authors： Gaur PK,El-Nakib O,Chu FS

更新日期：1980-09-01 00:00:00

abstract：:Nitrate was formed from ammonium at pH 3.2 to 6.1 in suspensions of a naturally acid forest soil; the maximum rates of formation occurred at ca. pH 4 to 5. Nitrate was also formed from soil nitrogen in suspensions incubated at 50 degrees C. Autotrophic nitrifying bacteria could not be isolated from this soil. Enrichme...

journal_title：Applied and environmental microbiology

authors： Stroo HF,Klein TM,Alexander M

更新日期：1986-11-01 00:00:00

abstract：:The effects of ethyl picolinate on germination, outgrowth, and sporulation of Bacillus cereus T were studied in a synthetic medium containing glucose. Ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. The initiation of germination and cell division was not affected. The inhibition of ou...

journal_title：Applied and environmental microbiology

authors： Pandey NK,Gollakota KG

更新日期：1977-05-01 00:00:00

abstract：:Increased listerial barotolerance at elevated osmolarity is attributed, in part, to the presence of accumulated betaine and L-carnitine. The percentage of listerial survival following exposure to 400 MPa for 5 min increased from 0.008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furth...

journal_title：Applied and environmental microbiology

authors： Smiddy M,Sleator RD,Patterson MF,Hill C,Kelly AL

更新日期：2004-12-01 00:00:00

abstract：:Species diversity, phylogenetic affiliations, and environmental occurrence patterns of thiosulfate-oxidizing marine bacteria were investigated by using new isolates from serially diluted continental slope and deep-sea abyssal plain sediments collected off the coast of New England and strains cultured previously from G...

journal_title：Applied and environmental microbiology

authors： Teske A,Brinkhoff T,Muyzer G,Moser DP,Rethmeier J,Jannasch HW

更新日期：2000-08-01 00:00:00

abstract：:In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicyla...

journal_title：Applied and environmental microbiology

authors： Randall LP,Woodward MJ

更新日期：2001-03-01 00:00:00

abstract：:Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat lab...

journal_title：Applied and environmental microbiology

authors： Ishibashi Y,Cervantes C,Silver S

更新日期：1990-07-01 00:00:00

abstract：:Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

journal_title：Applied and environmental microbiology

authors： Geng T,Morgan MT,Bhunia AK

更新日期：2004-10-01 00:00:00

abstract：:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

journal_title：Applied and environmental microbiology

authors： Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

更新日期：1995-06-01 00:00:00

abstract：:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

journal_title：Applied and environmental microbiology

authors： Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

更新日期：2005-08-01 00:00:00

abstract：:A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleoti...

journal_title：Applied and environmental microbiology

authors： Andrew M,Kohn LM

更新日期：2009-09-01 00:00:00

abstract：:Seventeen bacteriophages, active against 19 Arthrobacter soil isolates, were isolated from concentrated samples of river water and sewage. Attempts to isolate Arthrobacter bacteriophages from filtrates of broth cultures of the soil isolates or from ultraviolet light-irradiated cultures were unsuccessful. Bacteriophage...

journal_title：Applied and environmental microbiology

authors： Brown DR,Holt JG,Pattee PA

更新日期：1978-01-01 00:00:00

abstract：:Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...

journal_title：Applied and environmental microbiology

authors： Thomas CJ,McMeekin TA

更新日期：1981-02-01 00:00:00

abstract：:Stability of foreign DNA transformed into a novel host is an important parameter in decisions to permit the release of genetically engineered microorganisms into the environment. Meiotic instability of transformed DNA has been reported in fungi such as Ascobolus, Aspergillus, and Neurospora. We used strains of Gibbere...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Dickman MB

更新日期：1991-05-01 00:00:00

abstract：:Butyrate is an important intermediate in the anaerobic degradation of organic matter. In sulfate-depleted environments butyrate is oxidized to acetate and hydrogen by obligate proton reducers, in syntrophic association with hydrogen-consuming methanogens. This paper describes two enrichments of endospore-forming bacte...

journal_title：Applied and environmental microbiology

authors： Tomei FA,Maki JS,Mitchell R

更新日期：1985-11-01 00:00:00

abstract：:The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Oberleiter A,Psenner R

更新日期：1996-12-01 00:00:00

abstract：:The bile salt hydrolase activity in intestinal homogenates reflects composite activities of the gastrointestinal microbial consortia. We have proposed that specific transformations of conjugated bile acids by the intestinal microflora result in the production of metabolites which depress the growth of poultry. The inf...

journal_title：Applied and environmental microbiology

authors： Feighner SD,Dashkevicz MP

更新日期：1988-02-01 00:00:00

abstract：:The bacterial rhizosphere communities of three host plants of the pathogenic fungus Verticillium dahliae, field-grown strawberry (Fragaria ananassa Duch.), oilseed rape (Brassica napus L.), and potato (Solanum tuberosum L.), were analyzed. We aimed to determine the degree to which the rhizosphere effect is plant depen...

journal_title：Applied and environmental microbiology

authors： Smalla K,Wieland G,Buchner A,Zock A,Parzy J,Kaiser S,Roskot N,Heuer H,Berg G

更新日期：2001-10-01 00:00:00