Cloning of the Membrane-Bound Aldehyde Dehydrogenase Gene of Acetobacter polyoxogenes and Improvement of Acetic Acid Production by Use of the Cloned Gene.

abstract：:A stable shuttle vector which replicates in Escherichia coli and Clostridium perfringens was constructed by ligating a 3.6-kilobase (kb) fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 kb). The marker for this shuttle plasmid originated from the 1.3-kb chloramphenicol resistance gene of plasmid pHR1...

journal_title：Applied and environmental microbiology

authors： Kim AY,Blaschek HP

更新日期：1989-02-01 00:00:00

abstract：:A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...

journal_title：Applied and environmental microbiology

authors： Miyoshi T,Iwatsuki T,Naganuma T

更新日期：2005-02-01 00:00:00

abstract：:Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in the northwestern Arabian Sea to investigate the distribution of the organisms at var...

journal_title：Applied and environmental microbiology

authors： Sinninghe Damsté JS,Rijpstra WI,Hopmans EC,Prahl FG,Wakeham SG,Schouten S

更新日期：2002-06-01 00:00:00

abstract：:Cell extracts of Clostridium thermohydrosulfuricum, an anaerobic bacterium which ferments starch into ethanol at 65 degrees C, contained both pullulanase and glucoamylase activities. The general physiochemical and catalytic properties of these enzyme activities were compared. Pullulanase and glucoamylase activities we...

journal_title：Applied and environmental microbiology

authors： Hyun HH,Zeikus JG

更新日期：1985-05-01 00:00:00

abstract：:Fusarium oxysporum isolated from roots of and soil around Baccharis species from Brazil produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and 3'-OH T-2 (TC-1), whereas Fusarium sporotrichioides from the same source produced T-2 toxin, HT-2 toxin, acetyl T-2, neosolaniol, TC-1, 3'-OH HT-2 (TC-3), i...

journal_title：Applied and environmental microbiology

authors： Mirocha CJ,Abbas HK,Kommedahl T,Jarvis BB

更新日期：1989-01-01 00:00:00

abstract：:An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, ...

journal_title：Applied and environmental microbiology

authors： Dahling DR,Wright BA

更新日期：1986-04-01 00:00:00

abstract：:Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...

journal_title：Applied and environmental microbiology

authors： Reissbrodt R,Heier H,Tschäpe H,Kingsley RA,Williams PH

更新日期：2000-09-01 00:00:00

abstract：:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it t...

journal_title：Applied and environmental microbiology

authors： Egland PG,Gibson J,Harwood CS

更新日期：2001-03-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:[This corrects the article on p. 514 in vol. 55.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1989-06-01 00:00:00

abstract：:A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...

journal_title：Applied and environmental microbiology

authors： Yurkov V,Beatty JT

更新日期：1998-01-01 00:00:00

abstract：:Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

journal_title：Applied and environmental microbiology

authors： Khush RS,Becker E,Wach M

更新日期：1992-09-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:In this work, a strain of anaerobic pentachlorophenol (PCP) degrader, Desulfitobacterium frappieri PCP-1, was used to augment a mixed bacterial community of an anaerobic upflow sludge bed reactor degrading PCP. To estimate the efficiency of augmentation, the population of PCP-1 in the reactor was enumerated by a compe...

journal_title：Applied and environmental microbiology

authors： Tartakovsky B,Levesque M,Dumortier R,Beaudet R,Guiot SR

更新日期：1999-10-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U.S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin ge...

journal_title：Applied and environmental microbiology

authors： Xia X,Meng J,McDermott PF,Ayers S,Blickenstaff K,Tran TT,Abbott J,Zheng J,Zhao S

更新日期：2010-03-01 00:00:00

abstract：:Streptomyces coelicolor and Lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. S. coelicolor was grown in minimal medium with and without L. minor fronds. Bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparis...

journal_title：Applied and environmental microbiology

authors： Langlois P,Bourassa S,Poirier GG,Beaulieu C

更新日期：2003-04-01 00:00:00

abstract：:Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activit...

journal_title：Applied and environmental microbiology

authors： Mayer VW,Goin CJ

更新日期：1983-02-01 00:00:00

abstract：:Cyanobacteria can synthesize alkanes and alkenes, which are considered to be infrastructure-compatible biofuels. In terms of physiological function, cyanobacterial hydrocarbons are thought to be essential for membrane flexibility for cell division, size, and growth. The genetic basis for the biosynthesis of terminal o...

journal_title：Applied and environmental microbiology

authors： Zhu T,Scalvenzi T,Sassoon N,Lu X,Gugger M

更新日期：2018-06-18 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

journal_title：Applied and environmental microbiology

authors： Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

更新日期：2009-06-01 00:00:00

abstract：:A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

journal_title：Applied and environmental microbiology

authors： Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

更新日期：2015-12-01 00:00:00

abstract：:Salmonella is estimated to cause one million foodborne illnesses in the United States every year. Salmonella-contaminated poultry products are one of the major sources of salmonellosis. Given the critical role of the gut microbiota in Salmonella transmission, a manipulation of the chicken intestinal microenvironment c...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,Butz N,Cadenas MB,Koci M,Ballou A,Mendoza M,Ali R,Hassan H

更新日期：2018-02-14 00:00:00

abstract：:Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

journal_title：Applied and environmental microbiology

authors： Miller LK,Dawes KP

更新日期：1978-06-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:The electron donor cytochrome b5 (CybE/Cyb5) fuels the activity of the ergosterol biosynthesis-related P450 enzymes/P450s by providing electrons to P450s to promote ergosterol biosynthesis. Previous studies reported that lack of Aspergillus fumigatus (A. fumigatus) CybE reduces the proportion of ergosterol in total st...

journal_title：Applied and environmental microbiology

authors： Zhang C,Ren Y,Gao L,Gu H,Lu L

更新日期：2020-11-30 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00