Abstract:
:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it to 3-chlorobenzoyl coenzyme A (3-chlorobenzoyl-CoA), (ii) reductively dehalogenating 3-chlorobenzoyl-CoA to benzoyl-CoA, and (iii) degrading benzoyl-CoA to acetyl-CoA and carbon dioxide. R. palustris uses 3-chlorobenzoate only as a carbon source and thus incorporates the acetyl-CoA that is produced into cell material. The reductive dechlorination route used by R. palustris for 3-chlorobenzoate degradation differs from those previously described in that a CoA thioester, rather than an unmodified aromatic acid, is the substrate for complete dehalogenation.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Egland PG,Gibson J,Harwood CSdoi
10.1128/AEM.67.3.1396-1399.2001keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
1396-9issue
3eissn
0099-2240issn
1098-5336journal_volume
67pub_type
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