Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the nontranscribed spacer.

abstract：:Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...

journal_title：Applied and environmental microbiology

authors： González JM,Kiene RP,Moran MA

更新日期：1999-09-01 00:00:00

abstract：:A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

journal_title：Applied and environmental microbiology

authors： Visscher PT,Taylor BF

更新日期：1994-12-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

journal_title：Applied and environmental microbiology

authors： Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

更新日期：2012-08-01 00:00:00

abstract：:Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic microbes that are found in a broad range of aquatic environments. Although potentially significant to the microbial ecology and biogeochemistry of marine ecosystems, their abundance and genetic diversity and the environmental variables that regulate...

journal_title：Applied and environmental microbiology

authors： Ritchie AE,Johnson ZI

更新日期：2012-04-01 00:00:00

abstract：:Pseudomonas fluorescens 5R contains an NAH7-like plasmid (pKA1), and P. fluorescens 5R mutant 5RL contains a bioluminescent reporter plasmid (pUTK21) which was constructed by transposon mutagenesis. Polymerase chain reaction mapping confirmed the localization of lux transposon Tn4431 300 bp downstream from the start o...

journal_title：Applied and environmental microbiology

authors： Menn FM,Applegate BM,Sayler GS

更新日期：1993-06-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:A nonsporulating strain of Streptomyces diastaticus producing alpha-L-arabinofuranosidase activity (EC 3.2-1.55) was isolated from soil. Two alpha-L-arabinosidases were purified by ion-exchange chromatography and chromatofocusing. The enzymes had molecular weights of 38,000 (C1) and 60,000 (C2) and pIs of 8.8 and 8.3,...

journal_title：Applied and environmental microbiology

authors： Tajana E,Fiechter A,Zimmermann W

更新日期：1992-05-01 00:00:00

abstract：:Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

journal_title：Applied and environmental microbiology

authors： Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

更新日期：1999-12-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...

journal_title：Applied and environmental microbiology

authors： Xiong J,Wu L,Tu S,Van Nostrand JD,He Z,Zhou J,Wang G

更新日期：2010-11-01 00:00:00

abstract：:Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear ...

journal_title：Applied and environmental microbiology

authors： Barbirato F,Grivet JP,Soucaille P,Bories A

更新日期：1996-04-01 00:00:00

abstract：:Terminal olefins (1-alkenes) are natural products that have important industrial applications as both fuels and chemicals. However, their biosynthesis has been largely unexplored. We describe a group of bacteria, Jeotgalicoccus spp., which synthesize terminal olefins, in particular 18-methyl-1-nonadecene and 17-methyl...

journal_title：Applied and environmental microbiology

authors： Rude MA,Baron TS,Brubaker S,Alibhai M,Del Cardayre SB,Schirmer A

更新日期：2011-03-01 00:00:00

abstract：:Sodium gradients in cyanobacteria play an important role in energy storage under photoautotrophic conditions but have not been well studied during autofermentative metabolism under the dark, anoxic conditions widely used to produce precursors to fuels. Here we demonstrate significant stress-induced acceleration of aut...

journal_title：Applied and environmental microbiology

authors： Carrieri D,Ananyev G,Lenz O,Bryant DA,Dismukes GC

更新日期：2011-10-01 00:00:00

abstract：:Poultry are considered the major reservoir for Campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. To understand the ecology of C. jejuni and develop strategies to control C. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-Campylobacter mat...

journal_title：Applied and environmental microbiology

authors： Sahin O,Zhang Q,Meitzler JC,Harr BS,Morishita TY,Mohan R

更新日期：2001-09-01 00:00:00

abstract：:A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

journal_title：Applied and environmental microbiology

authors： Kannisto M,Aho T,Karp M,Santala V

更新日期：2014-11-01 00:00:00

abstract：:Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the ...

journal_title：Applied and environmental microbiology

authors： Lin X,Kennedy D,Peacock A,McKinley J,Resch CT,Fredrickson J,Konopka A

更新日期：2012-02-01 00:00:00

abstract：:Bacterioplankton are fundamental components of marine ecosystems and influence the entire biosphere by contributing to the global biogeochemical cycles of key elements. Yet, there is a significant gap in knowledge about their diversity and specific activities, as well as environmental factors that shape their communit...

journal_title：Applied and environmental microbiology

authors： Richa K,Balestra C,Piredda R,Benes V,Borra M,Passarelli A,Margiotta F,Saggiomo M,Biffali E,Sanges R,Scanlan DJ,Casotti R

更新日期：2017-08-17 00:00:00

abstract：:Molecular tools that can provide an estimate of the in situ growth rate of Geobacter species could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole-genome microarray analyses of a subsurface isolate of Geobacter uraniireducens, grown under a variety of conditions, identified...

journal_title：Applied and environmental microbiology

authors： Holmes DE,Giloteaux L,Barlett M,Chavan MA,Smith JA,Williams KH,Wilkins M,Long P,Lovley DR

更新日期：2013-03-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...

journal_title：Applied and environmental microbiology

authors： Tempelaars MH,Rodrigues S,Abee T

更新日期：2011-04-01 00:00:00

abstract：:We used a combination of stable isotope probing (SIP), gas chromatography-mass spectrometry-based respiration, isolation/cultivation, and quantitative PCR procedures to discover the identity and in situ growth of soil microorganisms that metabolize benzoic acid. We added [(13)C]benzoic acid or [(12)C]benzoic acid (100...

journal_title：Applied and environmental microbiology

authors： Pumphrey GM,Madsen EL

更新日期：2008-07-01 00:00:00

abstract：:Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

journal_title：Applied and environmental microbiology

authors： Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

更新日期：2020-05-19 00:00:00

abstract：:Currently, it is estimated that only 0.001% to 15% of bacteria in any given system can be cultured by use of commonly used techniques and media, yet culturing is critically important for investigations of bacterial function. Despite this situation, few studies have attempted to link culture-dependent and culture-indep...

journal_title：Applied and environmental microbiology

authors： Walke JB,Becker MH,Hughey MC,Swartwout MC,Jensen RV,Belden LK

更新日期：2015-10-01 00:00:00

abstract：:Cotton flowers in replicate plots in two fields near Phoenix, Ariz., were tagged in June at the beginning of the flowering period. Flowers or developing bolls from these tagged flowers were inoculated on the involucral (bracteal) nectaries with dry spores of Aspergillus flavus. The bolls were harvested as they matured...

journal_title：Applied and environmental microbiology

authors： Klich MA,Chmielewski MA

更新日期：1985-09-01 00:00:00

abstract：:A greenhouse experiment was conducted to determine the influence of two tropical isolates of Glomus fasciculatum and Glomus mosseae on the nutrient uptake and growth of Sesbania grandiflora. Inoculation of sterile soil with the fungi significantly improved growth and nutrient uptake by S. grandiflora, but the response...

journal_title：Applied and environmental microbiology

authors： Habte M,Aziz T

更新日期：1985-09-01 00:00:00

abstract：:In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

journal_title：Applied and environmental microbiology

authors： Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:Air drying of raw sludge caused inactivation of indigenous viruses. A gradual loss of infectivity occurred with the loss of water until the solids content reached about 80%. A more rapid decline of viral infectivity occurred with further dewatering. ...

journal_title：Applied and environmental microbiology

authors： Brashear DA,Ward RL

更新日期：1983-06-01 00:00:00

abstract：:Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

journal_title：Applied and environmental microbiology

authors： González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

更新日期：2006-01-01 00:00:00

abstract：:Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

journal_title：Applied and environmental microbiology

authors： Takiguchi Y,Yoshikawa H,Terao M

更新日期：1980-06-01 00:00:00

abstract：:An obligately anaerobic bacterium which transforms several chlorinated phenols was isolated. Dechlorination of the substituents ortho to the phenolic OH group was preferred, while removal of a meta-substituted chlorine was observed only with 3,5-dichlorophenol. The bacterium was a gram-positive, endospore-forming, mot...

journal_title：Applied and environmental microbiology

authors： Madsen T,Licht D

更新日期：1992-09-01 00:00:00