Abstract:
:DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 vector. The content of reactive oxygen species (ROS) in IM303 (I4) cells carrying pYGE was reduced to 31% of ROS content in the control cells with pUC 19. In the culture of wild-type strain, E. coli MM294, in the medium with paraquat (10 micromol/l), maximum specific growth rate of the cells with pYGE was about five times higher than that of the control cells, with a decreased ROS content in the former cells. The introduction of pYGE also suppressed the occurrence of the cells with altered amino acid requirement in the culture of MM294 cells with paraquat.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya Mdoi
10.1128/AEM.71.5.2762-2765.2005keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
2762-5issue
5eissn
0099-2240issn
1098-5336pii
71/5/2762journal_volume
71pub_type
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