Substrate-velocity relationships for the Trichoderma viride cellulase-catalyzed hydrolysis of cellulose.

abstract：:[This corrects the article on p. 2161 in vol. 59.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1993-12-01 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide n...

journal_title：Applied and environmental microbiology

authors： Fayed B,Ashford DA,Hashem AM,Amin MA,El Gazayerly ON,Gregory MA,Smith MC

更新日期：2015-12-01 00:00:00

abstract：:Myxococcus xanthus is a gram-negative soil bacterium best known for its remarkable life history of social swarming, social predation, and multicellular fruiting body formation. Very little is known about genetic diversity within this species or how social strategies might vary among neighboring strains at small spatia...

journal_title：Applied and environmental microbiology

authors： Vos M,Velicer GJ

更新日期：2006-05-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

journal_title：Applied and environmental microbiology

authors： Levy-Booth DJ,Winder RS

更新日期：2010-11-01 00:00:00

abstract：:ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

journal_title：Applied and environmental microbiology

authors： Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

更新日期：2010-05-01 00:00:00

abstract：:The successful nodulation of legumes by a Rhizobium strain is determined by the competitive ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in a...

journal_title：Applied and environmental microbiology

authors： Moawad H,Bohlool BB

更新日期：1984-07-01 00:00:00

abstract：:Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme responsible for inul...

journal_title：Applied and environmental microbiology

authors： Wang SA,Li FL

更新日期：2013-01-01 00:00:00

abstract：:A 5' nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose activity was modulated...

journal_title：Applied and environmental microbiology

authors： Norton DM,Batt CA

更新日期：1999-05-01 00:00:00

abstract：:The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...

journal_title：Applied and environmental microbiology

authors： Martinez-Urtaza J,Saco M,de Novoa J,Perez-Piñeiro P,Peiteado J,Lozano-Leon A,Garcia-Martin O

更新日期：2004-04-01 00:00:00

abstract：:Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were...

journal_title：Applied and environmental microbiology

authors： Klaassens ES,Boesten RJ,Haarman M,Knol J,Schuren FH,Vaughan EE,de Vos WM

更新日期：2009-05-01 00:00:00

abstract：:The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility of horizontal spread of resistance genes or the rapid emergence of multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed plasmids and host ce...

journal_title：Applied and environmental microbiology

authors： Dong WR,Xiang LX,Shao JZ

更新日期：2010-04-01 00:00:00

abstract：:Flies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence of Escherichia coli strains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the res...

journal_title：Applied and environmental microbiology

authors： Solà-Ginés M,González-López JJ,Cameron-Veas K,Piedra-Carrasco N,Cerdà-Cuéllar M,Migura-Garcia L

更新日期：2015-06-01 00:00:00

abstract：:Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater and seawater samples taken in Florida, the Gulf of Mexico, and the Bahamas Bank. Recoveries of T2 phage and calf thymus DNA added to artificial seawater and concentrated by VFF were 72.8 and 80%, respectively. Virus concentr...

journal_title：Applied and environmental microbiology

authors： Paul JH,Jiang SC,Rose JB

更新日期：1991-08-01 00:00:00

abstract：:Bovine enteroviruses belong to the family Picornaviridae. Little is known about their pathogenic potential; however, they cause asymptomatic infections in cattle and are excreted in feces. In the present study, viruses isolated from environmental samples were sequenced. According to phylogenetic analyses and standard ...

journal_title：Applied and environmental microbiology

authors： Shaukat S,Angez M,Alam MM,Sharif S,Khurshid A,Malik F,Rana MS,Mahmood T,Zaidi SS

更新日期：2012-06-01 00:00:00

abstract：:The efficiency of a system developed for the recovery of viruses contaminating large quantities of vegetables was investigated in the laboratory and tested in the field. Viruses seeded onto a number of leafy vegetables in the laboratory were eluted with a phosphate-buffered saline solution (pH 9.0). The eluate was cla...

journal_title：Applied and environmental microbiology

authors： Ward BK,Chenoweth CM,Irving LG

更新日期：1982-12-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limi...

journal_title：Applied and environmental microbiology

authors： Olstorpe M,Schnürer J,Passoth V

更新日期：2012-01-01 00:00:00

abstract：:Actinomycetes secrete into their surroundings a suite of enzymes involved in the biodegradation of plant lignocellulose; these have been reported to include both hydrolytic and oxidative enzymes, including peroxidases. Reports of secreted peroxidases have been based upon observations of peroxidase-like activity associ...

journal_title：Applied and environmental microbiology

authors： Mason MG,Ball AS,Reeder BJ,Silkstone G,Nicholls P,Wilson MT

更新日期：2001-10-01 00:00:00

abstract：:A seasonal study of the distribution of lysogenic bacteria in Tampa Bay, Florida, was conducted over a 13-month period. Biweekly water samples were collected and either were left unaltered or had the viral population reduced by filtration (pore size, 0.2 micro m) and resuspension in filtered (pore size, 0.2 micro m) w...

journal_title：Applied and environmental microbiology

authors： Williamson SJ,Houchin LA,McDaniel L,Paul JH

更新日期：2002-09-01 00:00:00

abstract：:The oxidation of fluorene, a polycyclic hydrocarbon which is not a substrate for fungal lignin peroxidase, was studied in liquid cultures of Phanerochaete chrysosporium and in vitro with P. chrysosporium extracellular enzymes. Intact fungal cultures metabolized fluorene to 9-hydroxyfluorene via 9-fluorenone. Some conv...

journal_title：Applied and environmental microbiology

authors： Bogan BW,Lamar RT,Hammel KE

更新日期：1996-05-01 00:00:00

abstract：:Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, w...

journal_title：Applied and environmental microbiology

authors： Littel KJ,Hartman PA

更新日期：1983-02-01 00:00:00

abstract：:The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

journal_title：Applied and environmental microbiology

authors： Jawson MD,Franzluebbers AJ,Berg RK

更新日期：1989-03-01 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00

abstract：:A gene fragment belonging to the cytochrome P-450 superfamily has been cloned and identified from stationary cultures of the filamentous fungus Phanerochaete chrysosporium by reverse transcriptase (RT)-PCR. A set of degenerate primers homologous to highly conserved regions of known cytochrome P-450 sequences were used...

journal_title：Applied and environmental microbiology

authors： Kullman SW,Matsumura F

更新日期：1997-07-01 00:00:00

abstract：:Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

journal_title：Applied and environmental microbiology

authors： Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

更新日期：1997-01-01 00:00:00

abstract：:An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microsc...

journal_title：Applied and environmental microbiology

authors： Bracke JW,Markovetz AJ

更新日期：1978-01-01 00:00:00

abstract：:Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...

journal_title：Applied and environmental microbiology

authors： Waterfield N,Dowling A,Sharma S,Daborn PJ,Potter U,Ffrench-Constant RH

更新日期：2001-11-01 00:00:00

abstract：:Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

journal_title：Applied and environmental microbiology

authors： McGowan C,Fulthorpe R,Wright A,Tiedje JM

更新日期：1998-10-01 00:00:00