Abstract:
:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid recombinase gene. We increased the production and secretion of two heterologous proteins, glucoamylase from the yeast Arxula adeninivorans and mammalian interleukin-1beta, following gene dosage amplification when the heterologous genes were carried by pKD1-based vectors. The choice of the promoters for expression of the integrated recombinase gene and of the episomal heterologous genes are critical for the mitotic stability of the host-vector system.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MMdoi
10.1128/AEM.65.11.4808-4813.1999keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
4808-13issue
11eissn
0099-2240issn
1098-5336journal_volume
65pub_type
杂志文章abstract::The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...
journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:1978-04-01 00:00:00
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更新日期:1985-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.64.11.4603-4606.1998
更新日期:1998-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.1.30-35.1980
更新日期:1980-01-01 00:00:00
abstract::In the present work, we aimed at determining the relationship between the hepatitis A virus (HAV) numbers in imported frozen coquina clams involved in two hepatitis outbreaks, as well as the risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling the vir...
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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更新日期:2020-03-18 00:00:00
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更新日期:2008-05-01 00:00:00
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更新日期:2010-10-01 00:00:00
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更新日期:2008-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.1685-1693.2005
更新日期:2005-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.6.2478-2484.1999
更新日期:1999-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00