Isolation of Azospirillum lipoferum 4T Tn5 Mutants Affected in Melanization and Laccase Activity.

abstract：:Psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in Antarctica have been isolated. Phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma Proteobacteria and the Flavobacteria-Cytophaga group. This is the first report ...

journal_title：Applied and environmental microbiology

authors： Gosink JJ,Staley JT

更新日期：1995-09-01 00:00:00

abstract：:A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

journal_title：Applied and environmental microbiology

authors： Lynch NA,Jiang H,Gibson DT

更新日期：1996-06-01 00:00:00

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for th...

journal_title：Applied and environmental microbiology

authors： Satoh W,Nakata M,Yamamoto H,Ezaki T,Hiramatsu K

更新日期：2002-12-01 00:00:00

abstract：:The respiration percentage of H-labeled glucose by heterotrophic microorganisms in water samples was determined by a method based on equimolar addition of [H]- and [C]glucose to samples. The respiration percentage of [H]glucose exceeded that of [C]glucose by 10% over the assayed range of respiration rates. Respiration...

journal_title：Applied and environmental microbiology

authors： Kuparinen J,Tamminen T

更新日期：1982-04-01 00:00:00

abstract：:A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...

journal_title：Applied and environmental microbiology

authors： Dreyfus BL,Elmerich C,Dommergues YR

更新日期：1983-02-01 00:00:00

abstract：:DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in solution. In addition, PNA molecular beacon hybridizations were found to be useful for the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA concentrations used in this experiment (...

journal_title：Applied and environmental microbiology

authors： Xi C,Balberg M,Boppart SA,Raskin L

更新日期：2003-09-01 00:00:00

abstract：:Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

journal_title：Applied and environmental microbiology

authors： Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

更新日期：1997-01-01 00:00:00

abstract：:Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

journal_title：Applied and environmental microbiology

authors： Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

更新日期：1990-08-01 00:00:00

abstract：:The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kina...

journal_title：Applied and environmental microbiology

authors： Kato J,Yamada K,Muramatsu A,Hardoyo,Ohtake H

更新日期：1993-11-01 00:00:00

abstract：:1,2-Dibromoethane (EDB), which is a groundwater contaminant in areas where it was once used as a soil fumigant, was shown to be degraded aerobically by microorganisms in two types of surface soils from an EDB-contaminated groundwater discharge area. At initial concentrations of 6 to 8 mug/liter, EDB was degraded in a ...

journal_title：Applied and environmental microbiology

authors： Pignatello JJ

更新日期：1986-03-01 00:00:00

abstract：:We recently showed that expressing an H(2)O-NADH oxidase in Saccharomyces cerevisiae drastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accu...

journal_title：Applied and environmental microbiology

authors： Heux S,Sablayrolles JM,Cachon R,Dequin S

更新日期：2006-09-01 00:00:00

abstract：:The frequency of recovery of atypical mycobacteria was estimated in two treatment plants providing drinking water to Paris, France, at some intermediate stages of treatment. The two plants use two different filtration processes, rapid and slow sand filtration. Our results suggest that slow sand filtration is more effi...

journal_title：Applied and environmental microbiology

authors： Le Dantec C,Duguet JP,Montiel A,Dumoutier N,Dubrou S,Vincent V

更新日期：2002-11-01 00:00:00

abstract：:Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a so...

journal_title：Applied and environmental microbiology

authors： Papp K,Hungate BA,Schwartz E

更新日期：2018-04-02 00:00:00

abstract：:Thirty presumptive Aeromonas hydrophila isolates were collected from estuarine sources using Rimler-Shotts media. Many of the isolates, especially the strains from a low-salinity site, were also identified as A. hydrophila using the API 20E system and two differential techniques devised for the identification of A. hy...

journal_title：Applied and environmental microbiology

authors： Davis JW,Sizemore RK

更新日期：1981-09-01 00:00:00

abstract：:Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...

journal_title：Applied and environmental microbiology

authors： Stamps BW,Nunn HS,Petryshyn VA,Oremland RS,Miller LG,Rosen MR,Bauer KW,Thompson KJ,Tookmanian EM,Waldeck AR,Loyd SJ,Johnson HA,Stevenson BS,Berelson WM,Corsetti FA,Spear JR

更新日期：2018-10-17 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleoti...

journal_title：Applied and environmental microbiology

authors： Andrew M,Kohn LM

更新日期：2009-09-01 00:00:00

abstract：:Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...

journal_title：Applied and environmental microbiology

authors： Kolkman MA,van der Ploeg R,Bertels M,van Dijk M,van der Laan J,van Dijl JM,Ferrari E

更新日期：2008-12-01 00:00:00

abstract：:Veratryl alcohol, added as a supplement to cultures of Phanerochaete chrysosporium, enhanced ligninase activity through protection of the ligninase against inactivation by hydrogen peroxide produced by this fungus in cultures. In the presence of veratryl alcohol, the loss of ligninase activity observed in non-protein-...

journal_title：Applied and environmental microbiology

authors： Tonon F,Odier E

更新日期：1988-02-01 00:00:00

abstract：:Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. S...

journal_title：Applied and environmental microbiology

authors： Panke S,de Lorenzo V,Kaiser A,Witholt B,Wubbolts MG

更新日期：1999-12-01 00:00:00

abstract：:Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

journal_title：Applied and environmental microbiology

authors： Gilliland SE,Speck ML

更新日期：1977-01-01 00:00:00

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of Alcaligenes faecalis. The genes required for oxidation of hypophosphi...

journal_title：Applied and environmental microbiology

authors： Wilson MM,Metcalf WW

更新日期：2005-01-01 00:00:00

abstract：:Factors affecting the production of [14C]patulin from [1-14C]acetate by replacement cultures of Penicillium patulum have been investigated. Incorporation of [1-14C]acetate into patulin reached a maximum with 6- to 8-day-old cultures incubated at 28 degrees C for 8 h in a replacement medium containing 0.1 M glucose, in...

journal_title：Applied and environmental microbiology

authors： Nip WK,Chu FS

更新日期：1977-04-01 00:00:00

abstract：:Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

journal_title：Applied and environmental microbiology

authors： Matsui GY,Ringelberg DB,Lovell CR

更新日期：2004-12-01 00:00:00

abstract：:Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

journal_title：Applied and environmental microbiology

authors： Ji P,Wilson M

更新日期：2003-02-01 00:00:00

abstract：:Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some...

journal_title：Applied and environmental microbiology

authors： Dorsey CW,Tomaras AP,Actis LA

更新日期：2002-12-01 00:00:00

abstract：:The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...

journal_title：Applied and environmental microbiology

authors： Widmer G,Lee Y

更新日期：2010-10-01 00:00:00

abstract：:In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and c...

journal_title：Applied and environmental microbiology

authors： Forquin MP,Hébert A,Roux A,Aubert J,Proux C,Heilier JF,Landaud S,Junot C,Bonnarme P,Martin-Verstraete I

更新日期：2011-02-01 00:00:00