Abstract:
:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two categories. Members of the tribe Klebsielleae were shown to have a prolonged survival rate of 40% or better after 24 h, whereas nonsurvivors were not viable for much longer than 24 h. These nonsurvivors belonged to the genera Acinetobacter, Aeromonas, Alcaligenes, Erwinia, Escherichia, Flavobacterium, and Pseudomonas. Differences in ribonuclease and adenosine triphosphatase levels between Escherichia coli (nonsurvivor) and Klebsiella (survivor) cells were detected. At pH 7.5, stressed E. coli cells contained 14% of the adenosine triphosphatase activity detected in the control, whereas at pH 5.5, in the presence of calcium ions, these same cells contained 50% of the control adenosine triphosphatase levels. At pH 7.2, E. coli cells were strongly inhibited by an adenosine triphosphatase inhibitor, bathophenanthroline (88%); oligomycin (64%); and the proton ionophore carbonyl- cyanide-m-chlorophenyl hydrazone (67%). Both sodium azide and valinomycin were only moderately inhibitory (15 and 28%, respectively). Although the ability to scavenge internal endogenous reserves seems important, we postulate that certain enteric bacteria are capable of utilizing acidic conditions (pH 5.5) as an electrochemical gradient to generate necessary high-energy intermediates for prolongation of survival beyond that possible in environments of near-neutraL pH.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Sjogren RE,Gibson MJdoi
10.1128/AEM.41.6.1331-1336.1981subject
Has Abstractpub_date
1981-06-01 00:00:00pages
1331-6issue
6eissn
0099-2240issn
1098-5336journal_volume
41pub_type
杂志文章abstract::In various sediments in which Fe(III) reduction was the terminal electron-accepting process, [C]glucose was fermented to C-fatty acids in a manner similar to that observed in methanogenic sediments. These results are consistent with the hypothesis that, in Fe(III)-reducing sediments, fermentable substrates are oxidize...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.12.3234-3236.1989
更新日期:1989-12-01 00:00:00
abstract::Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes in which there is high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a few UPO enzymes have been experi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02899-19
更新日期:2020-03-18 00:00:00
abstract::Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02187-15
更新日期:2015-11-01 00:00:00
abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4571-4574.2000
更新日期:2000-10-01 00:00:00
abstract::Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.7.1711-1713.1987
更新日期:1987-07-01 00:00:00
abstract::A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an induci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.3.561-566.1987
更新日期:1987-03-01 00:00:00
abstract::[This corrects the article on p. 662 in vol. 45.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.6.1964-1964.1983
更新日期:1983-06-01 00:00:00
abstract::Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.3.1210-1214.2005
更新日期:2005-03-01 00:00:00
abstract::Streptococcus thermophilus is a facultative anaerobic bacterium that has the ability to grow and survive in aerobic environments, but the mechanism for this remains unclear. In this study, the efeB gene, encoding a dye-decolorizing peroxidase, was identified in the genome of Streptococcus thermophilus CGMCC 7.179, and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01300-15
更新日期:2015-09-01 00:00:00
abstract::Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3385-3390.1996
更新日期:1996-09-01 00:00:00
abstract::Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01171-18
更新日期:2018-10-17 00:00:00
abstract::A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reduc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01658-07
更新日期:2008-03-01 00:00:00
abstract::The susceptibility of Trichoplusia ni larvae to several Bacillus thuringiensis insecticidal crystal proteins (ICPs) was tested. Neonatal larvae proved to be susceptible to solubilized trypsin-treated CryIA(a), CryIA(b), and CryIA(c) (50% lethal concentrations [LC(50)s], 570, 480, and 320 ng/cm, respectively) but showe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.10.3840-3846.1994
更新日期:1994-10-01 00:00:00
abstract::Viral pollution in shellfish has been analyzed simultaneously across a wide range of geographical regions, with emphasis on the concomitant variations in physicochemical characteristics and social features. The methods for sample treatment and for the detection of human enteric viruses were optimized by the participat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.12.5990-5998.2002
更新日期:2002-12-01 00:00:00
abstract::The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.5.1430-1436.1993
更新日期:1993-05-01 00:00:00
abstract::beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.6.1954-1956.1990
更新日期:1990-06-01 00:00:00
abstract::The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01095-10
更新日期:2010-10-01 00:00:00
abstract::The chlorosis-inducing phytotoxin coronatine is produced by several Pseudomonas syringae pathovars, including glycinea, morsprunorum, atropurpurea, and the closely related tomato and maculicola. To date, all coronatine-producing pv. glycinea, morsprunorum, and atropurpurea strains that have been examined carry the gen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.10.3530-3536.1995
更新日期:1995-10-01 00:00:00
abstract::Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite, has been suggested to have been a central part of the global biogeochemical nitrogen cycle since the oxygenation of Earth. The cultivation of several ammonia-oxidizing archaea (AOA) as well as the discovery that archaeal ammonia monooxygenase (amo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
doi:10.1128/AEM.01960-12
更新日期:2012-11-01 00:00:00
abstract::[This corrects the article on p. 711 in vol. 45.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.1.793-801.2006
更新日期:2006-01-01 00:00:00
abstract::The microbial transformation of [N-methyl-(sup14)C]aldicarb, a carbamate pesticide, occurred in aquifer, lake, and salt marsh sediments. Microbial degradation of aldicarb took place within 21 days in aquifer sediments from sites previously exposed to aldicarb (Jamesport, Long Island, N.Y.) but did not occur in sedimen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.8.2820-2829.1995
更新日期:1995-08-01 00:00:00
abstract::Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.1.440-443.2002
更新日期:2002-01-01 00:00:00
abstract::Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.6.1201-1205.1979
更新日期:1979-06-01 00:00:00
abstract::A total of 69 dyes were incorporated separately at different concentrations into an agar medium for evaluation of their effects on the quantitative recovery of five serotypes of Yersinia enterocolitica. One strain of Pseudomonas aeruginosa and one strain of Bacillus cereus were included for comparative purposes. Certa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.38.2.205-211.1979
更新日期:1979-08-01 00:00:00
abstract::The importance of viruses in aquatic ecosystem functioning has been widely described. However, few studies have examined tropical aquatic ecosystems. Here, we evaluated for the first time viruses and their relationship with other planktonic communities in an Amazonian freshwater ecosystem. Coupling between viruses and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01161-10
更新日期:2010-11-01 00:00:00
abstract::The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01529-07
更新日期:2008-02-01 00:00:00
abstract::Wood extractives, commonly referred to as pitch, cause major problems in the manufacturing of pulp and paper. Treatment of nonsterile southern yellow pine chips for 14 days with Pseudomonas fluorescens, Pseudomonas sp., Xanthomonas campestris, and Serratia marcescens reduced wood extractives by as much as 40%. Control...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.12.5201-5205.2000
更新日期:2000-12-01 00:00:00
abstract::Thiobacillus denitrificans is a widespread, chemolithoautotrophic bacterium with an unusual and environmentally relevant metabolic repertoire, which includes its ability to couple denitrification to sulfur compound oxidation; to catalyze anaerobic, nitrate-dependent oxidation of Fe(II) and U(IV); and to oxidize minera...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02928-06
更新日期:2007-05-01 00:00:00
abstract::Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01895-15
更新日期:2015-11-01 00:00:00