Purification and Characterization of an Autolysin from Clostridium acetobutylicum.

abstract：:The colonization of granular activated carbon columns by bacteria can have both beneficial and potentially detrimental consequences. Bacterial growth on the carbon surface can remove adsorbed organics and thus partially regenerate the carbon bed. However, growth can also increase the levels of bacteria in the column e...

journal_title：Applied and environmental microbiology

authors： Shimp RJ,Pfaender FK

更新日期：1982-08-01 00:00:00

abstract：:The internal pH of Clostridium acetobutylicum was determined at various stages during the growth of the organism. Even in the presence of significant quantities of acetic, butyric, and lactic acids, an internal pH of 6.2 was maintained. Experiments using N,N'-dicyclohexylcarbodiimide indicated that a functioning H+-AT...

journal_title：Applied and environmental microbiology

authors： Bowles LK,Ellefson WL

更新日期：1985-11-01 00:00:00

abstract：:Relatedness between Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis was assessed by Southern hybridization analysis, with cloned chromosomal genes as probes. The results indicate that strains of the two subspecies form two distinct groups and that the DNA sequence divergence between L. lactis subsp. lac...

journal_title：Applied and environmental microbiology

authors： Godon JJ,Delorme C,Ehrlich SD,Renault P

更新日期：1992-12-01 00:00:00

abstract：:Aerobic and anaerobic microorganisms were enumerated in tubercles collected from sections of the water distribution pipeline in the Columbus, Ohio, metropolitan area. Coliform bacteria were not detected in the tubercles examined. Sulfate-reducing bacteria were detected in 80% of the samples. Nitrate-reducing heterotro...

journal_title：Applied and environmental microbiology

authors： Tuovinen OH,Hsu JC

更新日期：1982-09-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

journal_title：Applied and environmental microbiology

authors： Yu EK,Saddler JN

更新日期：1983-09-01 00:00:00

abstract：:Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in...

journal_title：Applied and environmental microbiology

authors： Wegkamp A,Starrenburg M,de Vos WM,Hugenholtz J,Sybesma W

更新日期：2004-05-01 00:00:00

abstract：:Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

journal_title：Applied and environmental microbiology

authors： Santiago-Mercado J,Hazen TC

更新日期：1987-12-01 00:00:00

abstract：:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

更新日期：2004-08-01 00:00:00

abstract：:Microcosms were prepared from aquifer material, spiked with monoaromatic hydrocarbons, and amended with oxygen, nitrate, and nitrous oxide. Benzene and alkylbenzenes were degraded to concentrations below 5 micrograms/liter within 7 days under aerobic conditions, whereas only the alkylbenzenes were degraded when either...

journal_title：Applied and environmental microbiology

authors： Hutchins SR

更新日期：1991-08-01 00:00:00

abstract：:A long-term epidemiological study of Cryptosporidium molnari in aquacultured European sea bass (ESB) and gilthead sea bream (GSB) was performed in different types of facilities on the Atlantic, Cantabric, and Mediterranean coasts. Four types of studies were carried out. In study A, fish raised from juveniles to market...

journal_title：Applied and environmental microbiology

authors： Sitjà-Bobadilla A,Padrós F,Aguilera C,Alvarez-Pellitero P

更新日期：2005-01-01 00:00:00

abstract：:A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

journal_title：Applied and environmental microbiology

authors： Delécluse A,Rosso ML,Ragni A

更新日期：1995-12-01 00:00:00

abstract：:Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

journal_title：Applied and environmental microbiology

authors： Kim JS,Kim JW,Kathariou S

更新日期：2008-10-01 00:00:00

abstract：:Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

journal_title：Applied and environmental microbiology

authors： Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

更新日期：2006-04-01 00:00:00

abstract：:Of the toxins produced by Bacillus cereus, the emetic toxin is likely the most dangerous but, due to the lack of a suitable assay, the least well known. In this paper, a new, sensitive, inexpensive, and rapid bioassay for detection of the emetic toxin of B. cereus is described. The assay is based on the loss of motili...

journal_title：Applied and environmental microbiology

authors： Andersson MA,Mikkola R,Helin J,Andersson MC,Salkinoja-Salonen M

更新日期：1998-04-01 00:00:00

abstract：:We investigated associations of species of the Bacteroides fragilis group with Japanese cedar pollinosis (JCPsis). Cell numbers of Bacteroides fragilis and Bacteroides intestinalis were significantly higher in JCPsis subjects than in non-JCPsis subjects before the pollen season. They correlated positively with both sy...

journal_title：Applied and environmental microbiology

authors： Odamaki T,Xiao JZ,Sakamoto M,Kondo S,Yaeshima T,Iwatsuki K,Togashi H,Enomoto T,Benno Y

更新日期：2008-11-01 00:00:00

abstract：:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

journal_title：Applied and environmental microbiology

authors： Wells JM,Cole RJ,Cutler HC,Spalding DH

更新日期：1981-04-01 00:00:00

abstract：:Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as a putative levansucrase p...

journal_title：Applied and environmental microbiology

authors： Anwar MA,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2008-06-01 00:00:00

abstract：:Environmental surveillance is an effective approach in investigating circulating enteroviruses and had been conducted in the cities of Jinan and Linyi since February 2008 and April 2010, respectively. This study analyzed 46 sewage samples collected in the two cities in 2011 and found that echovirus 6 (E6) was the pred...

journal_title：Applied and environmental microbiology

authors： Tao Z,Song Y,Wang H,Zhang Y,Yoshida H,Ji S,Xu A,Song L,Liu Y,Cui N,Ji F,Li Y,Chen P,Xu W

更新日期：2012-10-01 00:00:00

abstract：:Contamination of soil and water with heavy metals and metalloids is a serious environmental problem. Cadmium and arsenic are major environmental contaminants that pose a serious threat to human health. Although toxicities of cadmium and arsenic to living organisms have been extensively studied, the molecular mechanism...

journal_title：Applied and environmental microbiology

authors： Techo T,Charoenpuntaweesin S,Auesukaree C

更新日期：2020-10-15 00:00:00

abstract：:A highly virulent Serratia proteamaculans strain, AGR96X, exhibiting specific pathogenicity against larvae of the New Zealand grass grub (Costelytra giveni; Coleoptera: Scarabaeidae) and the New Zealand manuka beetle (Pyronota festiva and P. setosa; Coleoptera: Scarabaeidae), was isolated from a diseased grass grub la...

journal_title：Applied and environmental microbiology

authors： Hurst MRH,Beattie A,Jones SA,Laugraud A,van Koten C,Harper L

更新日期：2018-05-01 00:00:00

abstract：:The purpose of this study was to examine host distribution patterns among fecal bacteria in the order Bacteroidales, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. We analyzed Bacteroidales 16S rRNA gene sequences from the feces of eight hosts: human, bovin...

journal_title：Applied and environmental microbiology

authors： Dick LK,Bernhard AE,Brodeur TJ,Santo Domingo JW,Simpson JM,Walters SP,Field KG

更新日期：2005-06-01 00:00:00

abstract：:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

journal_title：Applied and environmental microbiology

authors： Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

更新日期：1992-12-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00

abstract：:Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

journal_title：Applied and environmental microbiology

authors： Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

更新日期：2007-01-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Mycosporine-like amino acids (MAAs) are an important class of secondary metabolites known for their protection against UV radiation and other stress factors. Cyanobacteria produce a variety of MAAs, including shinorine, the active ingredient in many sunscreen creams. Bioinformatic analysis of the genome of the soil-dw...

journal_title：Applied and environmental microbiology

authors： Katoch M,Mazmouz R,Chau R,Pearson LA,Pickford R,Neilan BA

更新日期：2016-09-30 00:00:00

abstract：:The geochemical cycling of cobalt (Co) has often been considered to be controlled by the scavenging and oxidation of Co(II) on the surface of manganese [Mn(III,IV)] oxides or manganates. Because Mn(II) oxidation in the environment is often catalyzed by bacteria, we have investigated the ability of Mn(II)-oxidizing bac...

journal_title：Applied and environmental microbiology

authors： Lee Y,Tebo BM

更新日期：1994-08-01 00:00:00

abstract：:It was shown recently that recombinant Escherichia coli, defective in the beta-oxidation cycle and harboring a medium-chain-length (MCL) poly(3-hydroxyalkanoate) (PHA) polymerase-encoding gene of Pseudomonas, is able to produce MCL PHA from fatty acids but not from sugars or gluconate (S. Langenbach, B. H. A. Rehm, an...

journal_title：Applied and environmental microbiology

authors： Klinke S,Ren Q,Witholt B,Kessler B

更新日期：1999-02-01 00:00:00