Abstract:
:A new thermostable dipeptidase gene was cloned from the thermophile Brevibacillus borstelensis BCS-1 by genetic complementation of the D-Glu auxotroph Escherichia coli WM335 on a plate containing D-Ala-D-Glu. Nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid sequence with an M(r) of 35,000. The deduced amino acid sequence of the dipeptidase exhibited 52% similarity with the dipeptidase from Listeria monocytogenes. The enzyme was purified to homogeneity from recombinant E. coli WM335 harboring the dipeptidase gene from B. borstelensis BCS-1. Investigation of the enantioselectivity (E) to the P(1) and P(1)' site of Ala-Ala revealed that the ratio of the specificity constant (k(cat)/K(m)) for L-enantioselectivity to the P(1) site of Ala-Ala was 23.4 +/- 2.2 [E = (k(cat)/K(m))(L,D)/(k(cat)/K(m))(D,D)], while the D-enantioselectivity to the P(1)' site of Ala-Ala was 16.4 +/- 0.5 [E = (k(cat)/K(m))(L,D)/(k(cat)/K(m))(L,L)] at 55 degrees C. The enzyme was stable up to 55 degrees C, and the optimal pH and temperature were 8.5 and 65 degrees C, respectively. The enzyme was able to hydrolyze L-Asp-D-Ala, L-Asp-D-AlaOMe, Z-D-Ala-D-AlaOBzl, and Z-L-Asp-D-AlaOBzl, yet it could not hydrolyze D-Ala-L-Asp, D-Ala-L-Ala, D-AlaNH(2), and L-AlaNH(2.) The enzyme also exhibited beta-lactamase activity similar to that of a human renal dipeptidase. The dipeptidase successfully synthesized the precursor of the dipeptide sweetener Z-L-Asp-D-AlaOBzl.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Baek DH,Song JJ,Kwon SJ,Park C,Jung CM,Sung MHdoi
10.1128/aem.70.3.1570-1575.2004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
1570-5issue
3eissn
0099-2240issn
1098-5336journal_volume
70pub_type
杂志文章abstract::The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.05999-11
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doi:10.1128/AEM.02730-06
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doi:10.1128/AEM.02131-20
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.8.3683-3690.2002
更新日期:2002-08-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.5.1132-1137.1987
更新日期:1987-05-01 00:00:00
abstract::The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071, 1992). Genes fcbA and fcb...
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abstract::The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microco...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.2.316-322.1986
更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.70.9.5522-5527.2004
更新日期:2004-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.1.221-229.1996
更新日期:1996-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.4.1428-1433.1997
更新日期:1997-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.7.3243-3250.2002
更新日期:2002-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.3.804-808.1996
更新日期:1996-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.2.695-702.1998
更新日期:1998-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:2011-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.3.500-505.1984
更新日期:1984-03-01 00:00:00
abstract::Seawater used in mariculture has been suspected of being a potential source of infection. In this study, the lethal effects of low-amperage electric treatment on microorganisms were examined in natural seawater and in seawater inoculated with Vibrio parahaemolyticus. In both cases, bacteria including V. parahaemolytic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
abstract::Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章,随机对照试验
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更新日期:2014-02-01 00:00:00
abstract::Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02783-09
更新日期:2010-04-01 00:00:00
abstract::[This corrects the article on p. 2610 in vol. 57.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.2.780-780.1992
更新日期:1992-02-01 00:00:00
abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02056-10
更新日期:2010-12-01 00:00:00
abstract::A mathematical model was developed to estimate the number of logarithmic cycles (LDec) of lipopolysaccharide concentration destroyed by a dry-heat sterilization process. The LDec values calculated from the mathematical model agreed well with those obtained from the destruction of lipopolysaccharide by a dry-heat treat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.5.715-719.1978
更新日期:1978-11-01 00:00:00
abstract::We characterized the intracellular symbiotic microbiota of the bamboo pseudococcid Antonina crawii by performing a molecular phylogenetic analysis in combination with in situ hybridization. Almost the entire length of the bacterial 16S rRNA gene was amplified and cloned from A. crawii whole DNA. Restriction fragment l...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.2.643-650.2000
更新日期:2000-02-01 00:00:00