New Field Isolates of Rhizobium leguminosarum Biovar Viciae That Nodulate the Primitive Pea Cultivar Afghanistan in Addition to Modern Cultivars.

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as lon...

journal_title：Applied and environmental microbiology

authors： Whitby GE,Murray RG

更新日期：1980-01-01 00:00:00

abstract：:The human pathogen Vibrio vulnificus is the leading cause of seafood-related deaths in the United States. Strains are genotyped on the basis of alleles that correlate with isolation source, with clinical (C)-genotype strains being more often implicated in disease and environmental (E)-genotype strains being more frequ...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2015-09-01 00:00:00

abstract：:Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

journal_title：Applied and environmental microbiology

authors： Thar R,Kühl M,Holst G

更新日期：2001-06-01 00:00:00

abstract：:Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of ...

journal_title：Applied and environmental microbiology

authors： Jiang W,Wang Y,Luo J,Chen X,Zeng Y,Li X,Feng Z,Zhang L

更新日期：2020-07-02 00:00:00

abstract：:The microaerophilic nature of Campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. The deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (SOD). We have shown previously that Camp...

journal_title：Applied and environmental microbiology

authors： Purdy D,Cawthraw S,Dickinson JH,Newell DG,Park SF

更新日期：1999-06-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common gro...

journal_title：Applied and environmental microbiology

authors： Castle A,Speranzini D,Rghei N,Alm G,Rinker D,Bissett J

更新日期：1998-01-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked t...

journal_title：Applied and environmental microbiology

authors： Kobayashi T,Uchimura K,Deguchi S,Horikoshi K

更新日期：2012-04-01 00:00:00

abstract：:pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred from Streptococcus lactis to Bacillus subtilis. No conjugal transfer of pAM beta 1 from B. subtilis to S. lactis was observed. In addition, pAM beta 1 which had been reintroduced in S. lactis after cycling t...

journal_title：Applied and environmental microbiology

authors： van der Lelie D,Venema G

更新日期：1987-10-01 00:00:00

abstract：:Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...

journal_title：Applied and environmental microbiology

authors： Gursinsky T,Gröbe D,Schierhorn A,Jäger J,Andreesen JR,Söhling B

更新日期：2008-03-01 00:00:00

abstract：:In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...

journal_title：Applied and environmental microbiology

authors： Xiao Z,Bergeron H,Grosse S,Beauchemin M,Garron ML,Shaya D,Sulea T,Cygler M,Lau PC

更新日期：2008-02-01 00:00:00

abstract：:A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

更新日期：1998-07-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:This study investigated the differences in microbial community abundance, composition and diversity throughout the depth profiles in soils collected from corn and soybean fields in lowa, USA using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing...

journal_title：Applied and environmental microbiology

authors： Hao J,Chai YN,Lopes LD,Ordóñez RA,Wright EE,Archontoulis S,Schachtman DP

更新日期：2020-12-11 00:00:00

abstract：:The effects of water activity (aw, 0.955 to 0.970), pH (4.75 to 5.75), and storage time (up to 60 days) on toxin production by Clostridium botulinum in cooked, vacuum-packed potatoes were studied by using factorial design experiments and most-probable-number methodology. Samples were inoculated with 10(3), 10(4), or 1...

journal_title：Applied and environmental microbiology

authors： Dodds KL

更新日期：1989-03-01 00:00:00

abstract：:Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater and seawater samples taken in Florida, the Gulf of Mexico, and the Bahamas Bank. Recoveries of T2 phage and calf thymus DNA added to artificial seawater and concentrated by VFF were 72.8 and 80%, respectively. Virus concentr...

journal_title：Applied and environmental microbiology

authors： Paul JH,Jiang SC,Rose JB

更新日期：1991-08-01 00:00:00

abstract：:Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...

journal_title：Applied and environmental microbiology

authors： Li GQ,Li SS,Zhang ML,Wang J,Zhu L,Liang FL,Liu RL,Ma T

更新日期：2008-02-01 00:00:00

abstract：:A new strategy for the detection of infectious Cryptosporidium parvum oocysts in water samples, which combines immunomagnetic separation (IMS) for recovery of oocysts with in vitro cell culturing and PCR (CC-PCR), was field tested with a total of 122 raw source water samples and 121 filter backwash water grab samples ...

journal_title：Applied and environmental microbiology

authors： Di Giovanni GD,Hashemi FH,Shaw NJ,Abrams FA,LeChevallier MW,Abbaszadegan M

更新日期：1999-08-01 00:00:00

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ...

journal_title：Applied and environmental microbiology

authors： Miller EN,Jarboe LR,Yomano LP,York SW,Shanmugam KT,Ingram LO

更新日期：2009-07-01 00:00:00

abstract：:A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

journal_title：Applied and environmental microbiology

authors： Tellenbach C,Grünig CR,Sieber TN

更新日期：2010-09-01 00:00:00

abstract：:Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

journal_title：Applied and environmental microbiology

authors： Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

更新日期：2003-06-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:The contributions of various components of soil microflora and microfauna to rhizosphere phosphatase activity were determined with hydroponic cultures. Three treatments were employed: (i) plants alone (Bouteloua gracilis (H.B.K.) Lag. ex Steud.) (ii) plants plus bacteria (Pseudomonas sp.), and (iii) plants plus bacter...

journal_title：Applied and environmental microbiology

authors： Gould WD,Coleman DC,Rubink AJ

更新日期：1979-05-01 00:00:00

abstract：:A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide...

journal_title：Applied and environmental microbiology

authors： Martín M,Mengs G,Plaza E,Garbi C,Sánchez M,Gibello A,Gutierrez F,Ferrer E

更新日期：2000-03-01 00:00:00

abstract：:The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...

journal_title：Applied and environmental microbiology

authors： Park JH,Szponar B,Larsson L,Gold DR,Milton DK

更新日期：2004-01-01 00:00:00