Cloning and characterization of the lactococcal plasmid-encoded type II restriction/modification system, LlaDII.

abstract：:A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa mo...

journal_title：Applied and environmental microbiology

authors： Arif MI,Samin G,van Leeuwen JG,Oppentocht J,Janssen DB

更新日期：2012-09-01 00:00:00

abstract：:The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...

journal_title：Applied and environmental microbiology

authors： Bebien M,Chauvin JP,Adriano JM,Grosse S,Verméglio A

更新日期：2001-10-01 00:00:00

abstract：:A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to ...

journal_title：Applied and environmental microbiology

authors： Chapot-Chartier MP,Nardi M,Chopin MC,Chopin A,Gripon JC

更新日期：1993-01-01 00:00:00

abstract：:Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol...

journal_title：Applied and environmental microbiology

authors： Dikicioglu D,Pir P,Onsan ZI,Ulgen KO,Kirdar B,Oliver SG

更新日期：2008-09-01 00:00:00

abstract：:The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

journal_title：Applied and environmental microbiology

authors： Kadokura H,Yoda K,Imai M,Yamasaki M

更新日期：1990-09-01 00:00:00

abstract：:Although at least 13 antigenically distinct serotypes of Rhizobium leguminosarum bv. trifolii exist in an Abiqua silty clay loam soil, one serotype, AS6, occupies >/=50% of the root nodules formed on field-grown subclover and between 33 and 78% of the nodules formed on five annual clover species grown in the same soil...

journal_title：Applied and environmental microbiology

authors： Leung K,Yap K,Dashti N,Bottomley PJ

更新日期：1994-02-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:DysI is identified as the protein that confers specific immunity to dysgalacticin, a plasmid-encoded streptococcal bacteriocin. dysI is transcribed as part of the copG-repB-dysI replication-associated operon. DysI appears to function at the membrane level to prevent the inhibitory effects of dysgalacticin on glucose t...

journal_title：Applied and environmental microbiology

authors： Swe PM,Heng NC,Cook GM,Tagg JR,Jack RW

更新日期：2010-12-01 00:00:00

abstract：:Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains are closely related human pathogens that are responsible for food-borne epidemics in many countries. Integration host factor (IHF) and the locus of enterocyte effacement-encoded regulator (Ler) are needed for the expression o...

journal_title：Applied and environmental microbiology

authors： Li M,Rosenshine I,Tung SL,Wang XH,Friedberg D,Hew CL,Leung KY

更新日期：2004-09-01 00:00:00

abstract：:Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...

journal_title：Applied and environmental microbiology

authors： Simonet P,Bosco M,Chapelon C,Moiroud A,Normand P

更新日期：1994-04-01 00:00:00

abstract：:Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 isolated from pigs raised in conventional farms was previously reported. Here we report a study on 25 farms adhering to an alternative system. LA-MRSA ST398 was not detected in nasal swabs from 178 pigs or from 89 humans working and livin...

journal_title：Applied and environmental microbiology

authors： Cuny C,Friedrich AW,Witte W

更新日期：2012-02-01 00:00:00

abstract：:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences...

journal_title：Applied and environmental microbiology

authors： Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MB

更新日期：2013-09-01 00:00:00

abstract：:To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

journal_title：Applied and environmental microbiology

authors： Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

更新日期：2014-09-01 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00

abstract：:Direct electron microscopy of bacteriophages adsorbed to a carbon film without prior enrichment by specific host strains or concentration by physical or chemical methods was used to study the morphological diversity of natural bacteriophage assemblages in a North German lake. All samples contained a mixture of morphol...

journal_title：Applied and environmental microbiology

authors： Demuth J,Neve H,Witzel KP

更新日期：1993-10-01 00:00:00

abstract：:Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described. ...

journal_title：Applied and environmental microbiology

authors： Allcock ER,Reid SJ,Jones DT,Woods DR

更新日期：1982-03-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it t...

journal_title：Applied and environmental microbiology

authors： Egland PG,Gibson J,Harwood CS

更新日期：2001-03-01 00:00:00

abstract：:Fifty-one isolates of Rhizobium leguminosarum biovar phaseoli from various geographic and ecological sources, largely in Mexico, were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 46 distinctive multilocus genotypes (electrophoretic types [ETs]) were distinguished on the basis of allele ...

journal_title：Applied and environmental microbiology

authors： Pinero D,Martinez E,Selander RK

更新日期：1988-11-01 00:00:00

abstract：:Physical agents, such as low electric voltage and current, have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric current was shown to suppress the growth of bacteria, it remains unclear to what extent the microampere current damaged the bacterial...

journal_title：Applied and environmental microbiology

authors： Krishnamurthi VR,Rogers A,Peifer J,Niyonshuti II,Chen J,Wang Y

更新日期：2020-08-03 00:00:00

abstract：:We investigated the relationship between bacterioplankton production (BP), respiration (BR), and community composition measured by terminal restriction fragment length polymorphism in the southern North Sea over a seasonal cycle. Major changes in bacterioplankton richness were apparent from April to December. While ce...

journal_title：Applied and environmental microbiology

authors： Reinthaler T,Winter C,Herndl GJ

更新日期：2005-05-01 00:00:00

abstract：:An extracellular protein with strong absorption at 406 nm was purified from cell-free culture fluid of latex-grown Xanthomonas sp. strain 35Y. This protein was identical to the gene product of a recently characterized gene cloned from Xanthomonas sp., as revealed by determination of m/z values and sequencing of select...

journal_title：Applied and environmental microbiology

authors： Braaz R,Fischer P,Jendrossek D

更新日期：2004-12-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Fomites are known to play a role in the transmission of pathogens. Quantitative analysis of the parameters that affect sample recovery efficiency (SRE) at the limit of detection of viruses on fomites will aid in improving quantitative microbial risk assessment (QMRA) and infection control. The variability in SRE as a ...

journal_title：Applied and environmental microbiology

authors： Herzog AB,Pandey AK,Reyes-Gastelum D,Gerba CP,Rose JB,Hashsham SA

更新日期：2012-11-01 00:00:00

abstract：:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...

journal_title：Applied and environmental microbiology

authors： Davidson MS,Roy P,Summers AO

更新日期：1985-06-01 00:00:00

abstract：:Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of th...

journal_title：Applied and environmental microbiology

authors： Fassel TA,Buchholz LA,Collins ML,Remsen CC

更新日期：1992-07-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:The present investigation showed by means of autoradiography that the cyanobacterium Microcystis wesenbergii did not incorporate [H]thymidine at nanomolar concentrations, whereas its associated heterotrophic bacteria appearing in the gelatinous cover of the cyanobacterium became labeled. Several other tested cyaobacte...

journal_title：Applied and environmental microbiology

authors： Bern L

更新日期：1985-01-01 00:00:00

abstract：:Epilithic bacteria were isolated nonselectively from riverbed stones and examined by gel zymography for their ability to produce alkylsulfatase (AS) enzymes and thus to metabolize alkyl sulfate surfactants such as sodium dodecyl sulfate. The percentages of AS+ isolates from stone epilithon at five sites from the sourc...

journal_title：Applied and environmental microbiology

authors： Anderson DJ,Day MJ,Russell NJ,White GF

更新日期：1988-02-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00